Advanced search
1 file | 273.31 KB Add to list

MicroRNA expression analysis using small RNA sequencing discovery and RT-qPCR-based validation

Alan Van Goethem (UGent) , Pieter Mestdagh (UGent) , Tom Van Maerken (UGent) and Jo Vandesompele (UGent)
Author
Organization
Abstract
miRNAs are small noncoding RNA molecules that function as regulators of gene expression. Deregulated miRNA expression has been reported in various diseases including cancer. Due to their small size and high degree of homology, accurate quantification of miRNA expression is technically challenging. In this chapter, we present two different technologies for miRNA quantification: small RNA sequencing and RT-qPCR.
Keywords
miRNA, small RNA sequencing, RT-qPCR, miRNA annotation, normalization

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 273.31 KB

Citation

Please use this url to cite or link to this publication:

MLA
Van Goethem, Alan et al. “MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-based Validation.” Functional Genomics : Methods and Protocols. 3rd ed. Ed. Michael Kaufmann, Claudia Klinger, & Andreas Savelsbergh. Vol. 1654. New York, NY, USA: Springer Humana Press, 2017. 197–208. Print.
APA
Van Goethem, A., Mestdagh, P., Van Maerken, T., & Vandesompele, J. (2017). MicroRNA expression analysis using small RNA sequencing discovery and RT-qPCR-based validation. In M. Kaufmann, C. Klinger, & A. Savelsbergh (Eds.), Functional genomics : methods and protocols (3rd ed., Vol. 1654, pp. 197–208). New York, NY, USA: Springer Humana Press.
Chicago author-date
Van Goethem, Alan, Pieter Mestdagh, Tom Van Maerken, and Jo Vandesompele. 2017. “MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-based Validation.” In Functional Genomics : Methods and Protocols, ed. Michael Kaufmann, Claudia Klinger, and Andreas Savelsbergh, 1654:197–208. 3rd ed. New York, NY, USA: Springer Humana Press.
Chicago author-date (all authors)
Van Goethem, Alan, Pieter Mestdagh, Tom Van Maerken, and Jo Vandesompele. 2017. “MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-based Validation.” In Functional Genomics : Methods and Protocols, ed. Michael Kaufmann, Claudia Klinger, and Andreas Savelsbergh, 1654:197–208. 3rd ed. New York, NY, USA: Springer Humana Press.
Vancouver
1.
Van Goethem A, Mestdagh P, Van Maerken T, Vandesompele J. MicroRNA expression analysis using small RNA sequencing discovery and RT-qPCR-based validation. In: Kaufmann M, Klinger C, Savelsbergh A, editors. Functional genomics : methods and protocols. 3rd ed. New York, NY, USA: Springer Humana Press; 2017. p. 197–208.
IEEE
[1]
A. Van Goethem, P. Mestdagh, T. Van Maerken, and J. Vandesompele, “MicroRNA expression analysis using small RNA sequencing discovery and RT-qPCR-based validation,” in Functional genomics : methods and protocols, 3rd ed., vol. 1654, M. Kaufmann, C. Klinger, and A. Savelsbergh, Eds. New York, NY, USA: Springer Humana Press, 2017, pp. 197–208.
@incollection{8556842,
  abstract     = {miRNAs are small noncoding RNA molecules that function as regulators of gene expression. Deregulated miRNA expression has been reported in various diseases including cancer. Due to their small size and high degree of homology, accurate quantification of miRNA expression is technically challenging. In this chapter, we present two different technologies for miRNA quantification: small RNA sequencing and RT-qPCR.},
  author       = {Van Goethem, Alan and Mestdagh, Pieter and Van Maerken, Tom and Vandesompele, Jo},
  booktitle    = {Functional genomics : methods and protocols},
  editor       = {Kaufmann, Michael and Klinger, Claudia and Savelsbergh, Andreas},
  isbn         = {9781493972302},
  issn         = {1064-3745},
  keywords     = {miRNA,small RNA sequencing,RT-qPCR,miRNA annotation,normalization},
  language     = {eng},
  pages        = {197--208},
  publisher    = {Springer Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {MicroRNA expression analysis using small RNA sequencing discovery and RT-qPCR-based validation},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7231-9_13},
  volume       = {1654},
  year         = {2017},
}

Altmetric
View in Altmetric