Advanced search
1 file | 213.74 KB Add to list

In vitro assay for induction of adventitious rooting on intact Arabidopsis hypocotyls

Hoang Khai Trinh (UGent) , Inge Verstraeten (UGent) and Danny Geelen (UGent)
Author
Organization
Abstract
Adventitious roots (AR) are de novo formed roots that emerge from any part of the plant or from callus in tissue culture, except root tissue. The plant tissue origin and the method by which they are induced determine the physiological properties of emerged ARs. Hence, a standard method encompassing all types of AR does not exist. Here we describe a method for the induction and analysis of AR that emerge from the etiolated hypocotyl of dicot plants. The hypocotyl is formed during embryogenesis and shows a determined developmental pattern which usually does not involve AR formation. However, the hypocotyl shows propensity to form de novo roots under specific circumstances such as removal of the root system, high humidity or flooding, or during de-etiolation. The hypocotyl AR emerge from a pericycle-like cell layer surrounding the vascular tissue of the central cylinder, which is reminiscent to the developmental program of lateral roots. Here we propose an easy protocol for in vitro hypocotyl AR induction from etiolated Arabidopsis seedlings.
Keywords
Etiolated seedlings, Hypocotyls, Adventitious roots

Downloads

  • (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 213.74 KB

Citation

Please use this url to cite or link to this publication:

MLA
Trinh, Hoang Khai, et al. “In Vitro Assay for Induction of Adventitious Rooting on Intact Arabidopsis Hypocotyls.” Root Development : Methods and Protocols, edited by Daniela Ristova and Elke Barbez, vol. 1761, Humana, 2018, pp. 95–102, doi:10.1007/978-1-4939-7747-5_7.
APA
Trinh, H. K., Verstraeten, I., & Geelen, D. (2018). In vitro assay for induction of adventitious rooting on intact Arabidopsis hypocotyls. In D. Ristova & E. Barbez (Eds.), Root development : methods and protocols (Vol. 1761, pp. 95–102). https://doi.org/10.1007/978-1-4939-7747-5_7
Chicago author-date
Trinh, Hoang Khai, Inge Verstraeten, and Danny Geelen. 2018. “In Vitro Assay for Induction of Adventitious Rooting on Intact Arabidopsis Hypocotyls.” In Root Development : Methods and Protocols, edited by Daniela Ristova and Elke Barbez, 1761:95–102. New York, NY: Humana. https://doi.org/10.1007/978-1-4939-7747-5_7.
Chicago author-date (all authors)
Trinh, Hoang Khai, Inge Verstraeten, and Danny Geelen. 2018. “In Vitro Assay for Induction of Adventitious Rooting on Intact Arabidopsis Hypocotyls.” In Root Development : Methods and Protocols, ed by. Daniela Ristova and Elke Barbez, 1761:95–102. New York, NY: Humana. doi:10.1007/978-1-4939-7747-5_7.
Vancouver
1.
Trinh HK, Verstraeten I, Geelen D. In vitro assay for induction of adventitious rooting on intact Arabidopsis hypocotyls. In: Ristova D, Barbez E, editors. Root development : methods and protocols. New York, NY: Humana; 2018. p. 95–102.
IEEE
[1]
H. K. Trinh, I. Verstraeten, and D. Geelen, “In vitro assay for induction of adventitious rooting on intact Arabidopsis hypocotyls,” in Root development : methods and protocols, vol. 1761, D. Ristova and E. Barbez, Eds. New York, NY: Humana, 2018, pp. 95–102.
@incollection{8556442,
  abstract     = {{Adventitious roots (AR) are de novo formed roots that emerge from any part of the plant or from callus in tissue culture, except root tissue. The plant tissue origin and the method by which they are induced determine the physiological properties of emerged ARs. Hence, a standard method encompassing all types of AR does not exist. Here we describe a method for the induction and analysis of AR that emerge from the etiolated hypocotyl of dicot plants. The hypocotyl is formed during embryogenesis and shows a determined developmental pattern which usually does not involve AR formation. However, the hypocotyl shows propensity to form de novo roots under specific circumstances such as removal of the root system, high humidity or flooding, or during de-etiolation. The hypocotyl AR emerge from a pericycle-like cell layer surrounding the vascular tissue of the central cylinder, which is reminiscent to the developmental program of lateral roots. Here we propose an easy protocol for in vitro hypocotyl AR induction from etiolated Arabidopsis seedlings.}},
  author       = {{Trinh, Hoang Khai and Verstraeten, Inge and Geelen, Danny}},
  booktitle    = {{Root development : methods and protocols}},
  editor       = {{Ristova, Daniela and Barbez, Elke}},
  isbn         = {{9781493977468}},
  issn         = {{1064-3745}},
  keywords     = {{Etiolated seedlings,Hypocotyls,Adventitious roots}},
  language     = {{eng}},
  pages        = {{95--102}},
  publisher    = {{Humana}},
  series       = {{Methods in Molecular Biology}},
  title        = {{In vitro assay for induction of adventitious rooting on intact Arabidopsis hypocotyls}},
  url          = {{http://doi.org/10.1007/978-1-4939-7747-5_7}},
  volume       = {{1761}},
  year         = {{2018}},
}

Altmetric
View in Altmetric