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Nosema neumanni n. sp. (Microsporidia, Nosematidae), a new microsporidian parasite of honeybees, Apis mellifera in Uganda

Moses Chemurot (UGent) , Lina De Smet (UGent) , Marleen Brunain (UGent) , Riet De Rycke (UGent) and Dirk de Graaf (UGent)
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Abstract
The microsporidium Nosema neumanni n. sp., a new parasite of the honeybee Apis mellifera is described based on its ultra structural and molecular characteristics. Structures resembling microsporidian spores were found by microscopic examination of honeybees from Uganda. Molecular confirmation failed when PCR primers specific for Nosema apis and Nosema ceranae were used, but was successful with primers covering the whole family of Nosematidae. We performed transmission electron microscopy and found typical microsporidian spores which were smaller (length: 2.36 +/- 0.14 m and width: 1.78 +/- 0.06 p.m; n = 6) and had fewer polar filament coils (10-12) when compared to those of known species infecting honeybees. The entire 16S SS(J rRNA region was amplified, cloned and sequenced and was found to be unique with the highest resemblance (97% identity) to N. apis, The incidence of N. neumanni n. sp. in Ugandan honeybees was found to be much higher than of the two other Nosema species.
Keywords
CERANAE MICROSPORIDIA, BEE COLONIES, INFECTION, SEQUENCE, EUROPE, African honeybee, Microsporidia, Nosematidae, Nosemosis, 16 SSU RNA, Ultra-structure

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Chicago
Chemurot, Moses, Lina De Smet, Marleen Brunain, Riet De Rycke, and Dirk de Graaf. 2017. “Nosema Neumanni N. Sp. (Microsporidia, Nosematidae), a New Microsporidian Parasite of Honeybees, Apis Mellifera in Uganda.” European Journal of Protistology 61: 13–19.
APA
Chemurot, M., De Smet, L., Brunain, M., De Rycke, R., & de Graaf, D. (2017). Nosema neumanni n. sp. (Microsporidia, Nosematidae), a new microsporidian parasite of honeybees, Apis mellifera in Uganda. EUROPEAN JOURNAL OF PROTISTOLOGY, 61, 13–19.
Vancouver
1.
Chemurot M, De Smet L, Brunain M, De Rycke R, de Graaf D. Nosema neumanni n. sp. (Microsporidia, Nosematidae), a new microsporidian parasite of honeybees, Apis mellifera in Uganda. EUROPEAN JOURNAL OF PROTISTOLOGY. 2017;61:13–9.
MLA
Chemurot, Moses, Lina De Smet, Marleen Brunain, et al. “Nosema Neumanni N. Sp. (Microsporidia, Nosematidae), a New Microsporidian Parasite of Honeybees, Apis Mellifera in Uganda.” EUROPEAN JOURNAL OF PROTISTOLOGY 61 (2017): 13–19. Print.
@article{8556275,
  abstract     = {The microsporidium Nosema neumanni n. sp., a new parasite of the honeybee Apis mellifera is described based on its ultra structural and molecular characteristics. Structures resembling microsporidian spores were found by microscopic examination of honeybees from Uganda. Molecular confirmation failed when PCR primers specific for Nosema apis and Nosema ceranae were used, but was successful with primers covering the whole family of Nosematidae. We performed transmission electron microscopy and found typical microsporidian spores which were smaller (length: 2.36 +/- 0.14 m and width: 1.78 +/- 0.06 p.m; n = 6) and had fewer polar filament coils (10-12) when compared to those of known species infecting honeybees. The entire 16S SS(J rRNA region was amplified, cloned and sequenced and was found to be unique with the highest resemblance (97% identity) to N. apis, The incidence of N. neumanni n. sp. in Ugandan honeybees was found to be much higher than of the two other Nosema species.},
  author       = {Chemurot, Moses and De Smet, Lina and Brunain, Marleen and De Rycke, Riet and de Graaf, Dirk},
  issn         = {0932-4739},
  journal      = {EUROPEAN JOURNAL OF PROTISTOLOGY},
  keywords     = {CERANAE MICROSPORIDIA,BEE COLONIES,INFECTION,SEQUENCE,EUROPE,African honeybee,Microsporidia,Nosematidae,Nosemosis,16 SSU RNA,Ultra-structure},
  language     = {eng},
  pages        = {13--19},
  title        = {Nosema neumanni n. sp. (Microsporidia, Nosematidae), a new microsporidian parasite of honeybees, Apis mellifera in Uganda},
  url          = {http://dx.doi.org/10.1016/j.ejop.2017.07.002},
  volume       = {61},
  year         = {2017},
}

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