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Structural dissection of Shewanella oneidensis old yellow enzyme 4 bound to a Meisenheimer complex and (nitro)phenolic ligands

(2017) FEBS LETTERS. 591(20). p.3391-3401
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Abstract
Shewanella oneidensis, a Gram-negative -proteobacterium with an extensive redox capacity, possesses four old yellow enzyme (OYE) homologs. Of these, Shewanella yellow enzyme 4 (SYE4) is implicated in resistance to oxidative stress. Here, we present a series of high-resolution crystal structures for SYE4 in the oxidized and reduced states, and in complex with phenolic ligands and the nitro-aromatic explosive picric acid. The structures unmask new features, including the identification of a binding platform for long-chain hydrophobic molecules. Furthermore, we present the first structural observation of a hydride-Meisenheimer complex of picric acid with a flavoenzyme. Overall, our study exposes the binding promiscuity of SYE4 toward a variety of electrophilic substrates and is consistent with a general detoxification function for SYE4.
Keywords
PENTAERYTHRITOL TETRANITRATE REDUCTASE, ENTEROBACTER-CLOACAE PB2, X-RAY-STRUCTURE, ATOMIC-RESOLUTION, BACILLUS-SUBTILIS, SUBSTRATE-BINDING, OXIDATIVE STRESS, FATTY-ACIDS, SEQUENCE, HOMOLOG, atomic resolution crystallography, cellular detoxification, old yellow, enzyme, oxidative stress, redox chemistry

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Citation

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Chicago
Elegheert, Jonathan, Ann Brige, Jozef Van Beeumen, and Savvas Savvides. 2017. “Structural Dissection of Shewanella Oneidensis Old Yellow Enzyme 4 Bound to a Meisenheimer Complex and (nitro)phenolic Ligands.” Febs Letters 591 (20): 3391–3401.
APA
Elegheert, J., Brige, A., Van Beeumen, J., & Savvides, S. (2017). Structural dissection of Shewanella oneidensis old yellow enzyme 4 bound to a Meisenheimer complex and (nitro)phenolic ligands. FEBS LETTERS, 591(20), 3391–3401.
Vancouver
1.
Elegheert J, Brige A, Van Beeumen J, Savvides S. Structural dissection of Shewanella oneidensis old yellow enzyme 4 bound to a Meisenheimer complex and (nitro)phenolic ligands. FEBS LETTERS. 2017;591(20):3391–401.
MLA
Elegheert, Jonathan, Ann Brige, Jozef Van Beeumen, et al. “Structural Dissection of Shewanella Oneidensis Old Yellow Enzyme 4 Bound to a Meisenheimer Complex and (nitro)phenolic Ligands.” FEBS LETTERS 591.20 (2017): 3391–3401. Print.
@article{8556042,
  abstract     = {Shewanella oneidensis, a Gram-negative -proteobacterium with an extensive redox capacity, possesses four old yellow enzyme (OYE) homologs. Of these, Shewanella yellow enzyme 4 (SYE4) is implicated in resistance to oxidative stress. Here, we present a series of high-resolution crystal structures for SYE4 in the oxidized and reduced states, and in complex with phenolic ligands and the nitro-aromatic explosive picric acid. The structures unmask new features, including the identification of a binding platform for long-chain hydrophobic molecules. Furthermore, we present the first structural observation of a hydride-Meisenheimer complex of picric acid with a flavoenzyme. Overall, our study exposes the binding promiscuity of SYE4 toward a variety of electrophilic substrates and is consistent with a general detoxification function for SYE4.},
  author       = {Elegheert, Jonathan and Brige, Ann and Van Beeumen, Jozef and Savvides, Savvas},
  issn         = {1873-3468},
  journal      = {FEBS LETTERS},
  language     = {eng},
  number       = {20},
  pages        = {3391--3401},
  title        = {Structural dissection of Shewanella oneidensis old yellow enzyme 4 bound to a Meisenheimer complex and (nitro)phenolic ligands},
  url          = {http://dx.doi.org/10.1002/1873-3468.12833},
  volume       = {591},
  year         = {2017},
}

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