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CD169 is a marker for highly pathogenic phagocytes in multiple sclerosis

(2018) MULTIPLE SCLEROSIS JOURNAL. 24(3). p.290-300
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Abstract
Background: Phagocytes, such as macrophages and microglia, are key effector cells in the pathophysiology of multiple sclerosis (MS). It is widely accepted that they instigate and promote neuroinflammatory and neurodegenerative events in MS. An increasing amount of studies indicate that Siglec-1, also known CD169, is a marker for activated phagocytes in inflammatory disorders. Objective: In this study, we set out to define how CD169(+) phagocytes contribute to neuroinflammation in MS. Methods: CD169-diphtheria toxin receptor (DTR) mice, which express human DTR under control of the CD169 promoter, were used to define the impact of CD169(+) cells on neuroinflammation. Flow cytometry and immunohistochemistry were utilized to determine the expression and distribution of CD169. Results: We show that CD169 is highly expressed by lesional and circulating phagocytes in MS and experimental autoimmune encephalomyelitis (EAE). Our data further indicate that CD169 represents a selective marker for early activated microglia in MS and EAE lesions. Depletion of CD169(+) cells markedly reduced neuroinflammation and ameliorated disease symptoms in EAE-affected mice. Conclusion: Our findings indicate that CD169(+) cells promote neuroinflammation. Furthermore, they suggest that CD169(+) phagocytes play a key role in the pathophysiology of MS. Hence, targeting CD169(+) phagocytes may hold therapeutic value for MS.
Keywords
CD169, EAE, Siglec-1, microglia, monocytes, multiple sclerosis, neuroinflammation, sialoadhesin, ACID-BINDING-RECEPTOR, CIRCULATING MONOCYTES, EXPRESSION, SIALOADHESIN, MACROPHAGES, RESIDENT, SYSTEM, CELLS, POPULATIONS, INTERFERONS

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Chicago
Bogie, Jeroen FJ, Ellen Boelen, Els Louagie, Peter Delputte, Dirk Elewaut, Jack van Horssen, Jerome JA Hendriks, and Niels Hellings. 2018. “CD169 Is a Marker for Highly Pathogenic Phagocytes in Multiple Sclerosis.” Multiple Sclerosis Journal 24 (3): 290–300.
APA
Bogie, J. F., Boelen, E., Louagie, E., Delputte, P., Elewaut, D., van Horssen, J., Hendriks, J. J., et al. (2018). CD169 is a marker for highly pathogenic phagocytes in multiple sclerosis. MULTIPLE SCLEROSIS JOURNAL, 24(3), 290–300.
Vancouver
1.
Bogie JF, Boelen E, Louagie E, Delputte P, Elewaut D, van Horssen J, et al. CD169 is a marker for highly pathogenic phagocytes in multiple sclerosis. MULTIPLE SCLEROSIS JOURNAL. 2018;24(3):290–300.
MLA
Bogie, Jeroen FJ, Ellen Boelen, Els Louagie, et al. “CD169 Is a Marker for Highly Pathogenic Phagocytes in Multiple Sclerosis.” MULTIPLE SCLEROSIS JOURNAL 24.3 (2018): 290–300. Print.
@article{8555816,
  abstract     = {Background: Phagocytes, such as macrophages and microglia, are key effector cells in the pathophysiology of multiple sclerosis (MS). It is widely accepted that they instigate and promote neuroinflammatory and neurodegenerative events in MS. An increasing amount of studies indicate that Siglec-1, also known CD169, is a marker for activated phagocytes in inflammatory disorders. 
Objective: In this study, we set out to define how CD169(+) phagocytes contribute to neuroinflammation in MS. 
Methods: CD169-diphtheria toxin receptor (DTR) mice, which express human DTR under control of the CD169 promoter, were used to define the impact of CD169(+) cells on neuroinflammation. Flow cytometry and immunohistochemistry were utilized to determine the expression and distribution of CD169. 
Results: We show that CD169 is highly expressed by lesional and circulating phagocytes in MS and experimental autoimmune encephalomyelitis (EAE). Our data further indicate that CD169 represents a selective marker for early activated microglia in MS and EAE lesions. Depletion of CD169(+) cells markedly reduced neuroinflammation and ameliorated disease symptoms in EAE-affected mice. 
Conclusion: Our findings indicate that CD169(+) cells promote neuroinflammation. Furthermore, they suggest that CD169(+) phagocytes play a key role in the pathophysiology of MS. Hence, targeting CD169(+) phagocytes may hold therapeutic value for MS.},
  author       = {Bogie, Jeroen FJ and Boelen, Ellen and Louagie, Els and Delputte, Peter and Elewaut, Dirk and van Horssen, Jack and Hendriks, Jerome JA and Hellings, Niels},
  issn         = {1352-4585},
  journal      = {MULTIPLE SCLEROSIS JOURNAL},
  keyword      = {CD169,EAE,Siglec-1,microglia,monocytes,multiple sclerosis,neuroinflammation,sialoadhesin,ACID-BINDING-RECEPTOR,CIRCULATING MONOCYTES,EXPRESSION,SIALOADHESIN,MACROPHAGES,RESIDENT,SYSTEM,CELLS,POPULATIONS,INTERFERONS},
  language     = {eng},
  number       = {3},
  pages        = {290--300},
  title        = {CD169 is a marker for highly pathogenic phagocytes in multiple sclerosis},
  url          = {http://dx.doi.org/10.1177/1352458517698759},
  volume       = {24},
  year         = {2018},
}

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