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Isolation and identification of intestinal myeloid cells

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Abstract
The identification of conventional dendritic cells (cDCs) and macrophages (mϕ) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from each other and from other intestinal myeloid cells. Here we detail the protocols we have developed to isolate live leukocytes from steady state mouse small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs, monocytes and macrophages in such preparations.

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Chicago
Scott, Charlotte, Calum C Bain, and Allan McI Mowat. 2017. “Isolation and Identification of Intestinal Myeloid Cells.” In Inflammation : Methods and Protocols, ed. Björn E Clausen and Jon D Laman, 1559:223–239. New York, NY, USA: Humana Press.
APA
Scott, C., Bain, C. C., & Mowat, A. M. (2017). Isolation and identification of intestinal myeloid cells. In B. E. Clausen & J. D. Laman (Eds.), Inflammation : methods and protocols (Vol. 1559, pp. 223–239). New York, NY, USA: Humana Press.
Vancouver
1.
Scott C, Bain CC, Mowat AM. Isolation and identification of intestinal myeloid cells. In: Clausen BE, Laman JD, editors. Inflammation : methods and protocols. New York, NY, USA: Humana Press; 2017. p. 223–39.
MLA
Scott, Charlotte, Calum C Bain, and Allan McI Mowat. “Isolation and Identification of Intestinal Myeloid Cells.” Inflammation : Methods and Protocols. Ed. Björn E Clausen & Jon D Laman. Vol. 1559. New York, NY, USA: Humana Press, 2017. 223–239. Print.
@incollection{8555656,
  abstract     = {The identification of conventional dendritic cells (cDCs) and macrophages (mϕ) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from each other and from other intestinal myeloid cells. Here we detail the protocols we have developed to isolate live leukocytes from steady state mouse small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs, monocytes and macrophages in such preparations.},
  author       = {Scott, Charlotte and Bain, Calum C and Mowat, Allan McI},
  booktitle    = { Inflammation : methods and protocols},
  editor       = {Clausen, Björn E and Laman, Jon D},
  isbn         = {9781493967841},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {223--239},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Isolation and identification of intestinal myeloid cells},
  url          = {http://dx.doi.org/10.1007/978-1-4939-6786-5_15},
  volume       = {1559},
  year         = {2017},
}

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