Advanced search
1 file | 6.52 MB

Cancer cell lysate entrapment in CaCO3 engineered with polymeric TLR-agonists : immune-modulating microparticles in view of personalized antitumor vaccination

(2017) CHEMISTRY OF MATERIALS. 29(10). p.4209-4217
Author
Organization
Abstract
Here, we report on a strategy to encapsulate cancer cell lysate in immunomodulating microparticles in view of their potential use in personalized anticancer vaccination. In a first step, cancer cell lysate is entrapped into porous calcium carbonate (CaCO3) microparticles by a coprecipitation reaction. Second, a polymer substituted with a small molecule TLR7/8-agonist is adorbed onto the surface of the micro particles. Relative to soluble cell lysate, microparticle encapsulation yielded higher uptake of cell lysate by dendritic cells in vitro. Also, microparticle-adsorbed polymeric TLR7/8-agonists retained their TLR-triggering capacity resulting in immunogenic vaccine particles. We anticipate this strategy might hold potential to turn patient-derived tumor tissue, comprising the patient's own mutanome, into potent personalized anticancer vaccines.
Keywords
POLYELECTROLYTE MULTILAYER CAPSULES, DENDRITIC CELLS, PROTEIN, ENCAPSULATION, IMMUNOTHERAPY, VACCINES, NEOANTIGENS, ADJUVANTS, ANTIGENS, DELIVERY, DESIGN

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 6.52 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Lybaert, Lien, Keun Ah Ryu, Lutz Nuhn, Riet De Rycke, Olivier De Wever, Alfred C Chon, Aaron P Esser-Kahn, and Bruno De Geest. 2017. “Cancer Cell Lysate Entrapment in CaCO3 Engineered with Polymeric TLR-agonists : Immune-modulating Microparticles in View of Personalized Antitumor Vaccination.” Chemistry of Materials 29 (10): 4209–4217.
APA
Lybaert, L., Ryu, K. A., Nuhn, L., De Rycke, R., De Wever, O., Chon, A. C., Esser-Kahn, A. P., et al. (2017). Cancer cell lysate entrapment in CaCO3 engineered with polymeric TLR-agonists : immune-modulating microparticles in view of personalized antitumor vaccination. CHEMISTRY OF MATERIALS, 29(10), 4209–4217.
Vancouver
1.
Lybaert L, Ryu KA, Nuhn L, De Rycke R, De Wever O, Chon AC, et al. Cancer cell lysate entrapment in CaCO3 engineered with polymeric TLR-agonists : immune-modulating microparticles in view of personalized antitumor vaccination. CHEMISTRY OF MATERIALS. 2017;29(10):4209–17.
MLA
Lybaert, Lien, Keun Ah Ryu, Lutz Nuhn, et al. “Cancer Cell Lysate Entrapment in CaCO3 Engineered with Polymeric TLR-agonists : Immune-modulating Microparticles in View of Personalized Antitumor Vaccination.” CHEMISTRY OF MATERIALS 29.10 (2017): 4209–4217. Print.
@article{8555645,
  abstract     = {Here, we report on a strategy to encapsulate cancer cell lysate in immunomodulating microparticles in view of their potential use in personalized anticancer vaccination. In a first step, cancer cell lysate is entrapped into porous calcium carbonate (CaCO3) microparticles by a coprecipitation reaction. Second, a polymer substituted with a small molecule TLR7/8-agonist is adorbed onto the surface of the micro particles. Relative to soluble cell lysate, microparticle encapsulation yielded higher uptake of cell lysate by dendritic cells in vitro. Also, microparticle-adsorbed polymeric TLR7/8-agonists retained their TLR-triggering capacity resulting in immunogenic vaccine particles. We anticipate this strategy might hold potential to turn patient-derived tumor tissue, comprising the patient's own mutanome, into potent personalized anticancer vaccines.},
  author       = {Lybaert, Lien and Ryu, Keun Ah and Nuhn, Lutz and De Rycke, Riet and De Wever, Olivier and Chon, Alfred C and Esser-Kahn, Aaron P and De Geest, Bruno},
  issn         = {0897-4756},
  journal      = {CHEMISTRY OF MATERIALS},
  keywords     = {POLYELECTROLYTE MULTILAYER CAPSULES,DENDRITIC CELLS,PROTEIN,ENCAPSULATION,IMMUNOTHERAPY,VACCINES,NEOANTIGENS,ADJUVANTS,ANTIGENS,DELIVERY,DESIGN},
  language     = {eng},
  number       = {10},
  pages        = {4209--4217},
  title        = {Cancer cell lysate entrapment in CaCO3 engineered with polymeric TLR-agonists : immune-modulating microparticles in view of personalized antitumor vaccination},
  url          = {http://dx.doi.org/10.1021/acs.chemmater.6b05062},
  volume       = {29},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: