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Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction

Morgane Boone (UGent) , Andries De Koker (UGent) and Nico Callewaert (UGent)
(2018) NUCLEIC ACIDS RESEARCH. 46(6). p.2701-2721
Author
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Abstract
All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences. In the past decade especially, driven by the progress in the field of massively parallel sequencing, numerous studies have comprehensively assessed the impact of particular manipulations on library complexity and quality, and characterized the activities and specificities of several key enzymes used in library construction. Fortunately, careful protocol design and reagent choice can substantially mitigate many of these biases, and enable reliable representation of sequences in libraries. This review aims to guide the reader through the vast expanse of literature on the subject to promote informed library generation, independent of the application.
Keywords
DUPLEX-SPECIFIC NUCLEASE, GROUP-II INTRON, WHOLE-GENOME AMPLIFICATION, T4 POLYNUCLEOTIDE KINASE, NORMALIZED CDNA LIBRARY, COMPLEX GENE LIBRARIES, PIWI-INTERACTING RNAS, CELL-FREE DNA, SINGLE-CELL, REVERSE-TRANSCRIPTASE

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Citation

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MLA
Boone, Morgane, Andries De Koker, and Nico Callewaert. “Capturing the ‘Ome’ : the Expanding Molecular Toolbox for RNA and DNA Library Construction.” NUCLEIC ACIDS RESEARCH 46.6 (2018): 2701–2721. Print.
APA
Boone, Morgane, De Koker, A., & Callewaert, N. (2018). Capturing the “ome” : the expanding molecular toolbox for RNA and DNA library construction. NUCLEIC ACIDS RESEARCH, 46(6), 2701–2721.
Chicago author-date
Boone, Morgane, Andries De Koker, and Nico Callewaert. 2018. “Capturing the ‘Ome’ : the Expanding Molecular Toolbox for RNA and DNA Library Construction.” Nucleic Acids Research 46 (6): 2701–2721.
Chicago author-date (all authors)
Boone, Morgane, Andries De Koker, and Nico Callewaert. 2018. “Capturing the ‘Ome’ : the Expanding Molecular Toolbox for RNA and DNA Library Construction.” Nucleic Acids Research 46 (6): 2701–2721.
Vancouver
1.
Boone M, De Koker A, Callewaert N. Capturing the “ome” : the expanding molecular toolbox for RNA and DNA library construction. NUCLEIC ACIDS RESEARCH. 2018;46(6):2701–21.
IEEE
[1]
M. Boone, A. De Koker, and N. Callewaert, “Capturing the ’ome’ : the expanding molecular toolbox for RNA and DNA library construction,” NUCLEIC ACIDS RESEARCH, vol. 46, no. 6, pp. 2701–2721, 2018.
@article{8554704,
  abstract     = {All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences. In the past decade especially, driven by the progress in the field of massively parallel sequencing, numerous studies have comprehensively assessed the impact of particular manipulations on library complexity and quality, and characterized the activities and specificities of several key enzymes used in library construction. Fortunately, careful protocol design and reagent choice can substantially mitigate many of these biases, and enable reliable representation of sequences in libraries. This review aims to guide the reader through the vast expanse of literature on the subject to promote informed library generation, independent of the application.},
  author       = {Boone, Morgane and De Koker, Andries and Callewaert, Nico},
  issn         = {0305-1048},
  journal      = {NUCLEIC ACIDS RESEARCH},
  keywords     = {DUPLEX-SPECIFIC NUCLEASE,GROUP-II INTRON,WHOLE-GENOME AMPLIFICATION,T4 POLYNUCLEOTIDE KINASE,NORMALIZED CDNA LIBRARY,COMPLEX GENE LIBRARIES,PIWI-INTERACTING RNAS,CELL-FREE DNA,SINGLE-CELL,REVERSE-TRANSCRIPTASE},
  language     = {eng},
  number       = {6},
  pages        = {2701--2721},
  title        = {Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction},
  url          = {http://dx.doi.org/10.1093/nar/gky167},
  volume       = {46},
  year         = {2018},
}

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