Advanced search
1 file | 2.58 MB

Osteopontin promotes protective antigenic tolerance against experimental allergic airway disease

(2018) JOURNAL OF IMMUNOLOGY. 200(4). p.1270-1282
Author
Organization
Abstract
In the context of inflammation, osteopontin (Opn) is known to promote effector responses, facilitating a proinflammatory environment; however, its role during antigenic tolerance induction is unknown. Using a mouse model of asthma, we investigated the role of Opn during antigenic tolerance induction and its effects on associated regulatory cellular populations prior to disease initiation. Our experiments demonstrate that Opn drives protective antigenic tolerance by inducing accumulation of IFN-beta-producing plasmacytoid dendritic cells, as well as regulatory T cells, in mediastinal lymph nodes. We also show that, in the absence of TLR triggers, recombinant Opn, and particularly its SLAYGLR motif, directly induces IFN-beta expression in Ag-primed plasmacytoid dendritic cells, which renders them extra protective against induction of allergic airway disease upon transfer into recipient mice. Lastly, we show that blockade of type I IFNR prevents antigenic tolerance induction against experimental allergic asthma. Overall, we unveil a new role for Opn in setting up a tolerogenic milieu boosting antigenic tolerance induction, thus leading to prevention of allergic airway inflammation. Our results provide insight for the future design of immunotherapies against allergic asthma.
Keywords
REGULATORY T-CELLS, PLASMACYTOID DENDRITIC CELLS, IN-VIVO, MEDIATED-IMMUNITY, TGF-BETA, ASTHMA, INFLAMMATION, RESPONSES, DIFFERENTIATION, EXPRESSION

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 2.58 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Alissafi, Themis, Evangelia Kourepini, Davina CM Simoes, Nikolaos Paschalidis, Maria Aggelakopoulou, Tim Sparwasser, Louis Boon, Hamida Hammad, Bart Lambrecht, and Vily Panoutsakopoulou. 2018. “Osteopontin Promotes Protective Antigenic Tolerance Against Experimental Allergic Airway Disease.” Journal of Immunology 200 (4): 1270–1282.
APA
Alissafi, T., Kourepini, E., Simoes, D. C., Paschalidis, N., Aggelakopoulou, M., Sparwasser, T., Boon, L., et al. (2018). Osteopontin promotes protective antigenic tolerance against experimental allergic airway disease. JOURNAL OF IMMUNOLOGY, 200(4), 1270–1282.
Vancouver
1.
Alissafi T, Kourepini E, Simoes DC, Paschalidis N, Aggelakopoulou M, Sparwasser T, et al. Osteopontin promotes protective antigenic tolerance against experimental allergic airway disease. JOURNAL OF IMMUNOLOGY. 2018;200(4):1270–82.
MLA
Alissafi, Themis et al. “Osteopontin Promotes Protective Antigenic Tolerance Against Experimental Allergic Airway Disease.” JOURNAL OF IMMUNOLOGY 200.4 (2018): 1270–1282. Print.
@article{8554321,
  abstract     = {In the context of inflammation, osteopontin (Opn) is known to promote effector responses, facilitating a proinflammatory environment; however, its role during antigenic tolerance induction is unknown. Using a mouse model of asthma, we investigated the role of Opn during antigenic tolerance induction and its effects on associated regulatory cellular populations prior to disease initiation. Our experiments demonstrate that Opn drives protective antigenic tolerance by inducing accumulation of IFN-beta-producing plasmacytoid dendritic cells, as well as regulatory T cells, in mediastinal lymph nodes. We also show that, in the absence of TLR triggers, recombinant Opn, and particularly its SLAYGLR motif, directly induces IFN-beta expression in Ag-primed plasmacytoid dendritic cells, which renders them extra protective against induction of allergic airway disease upon transfer into recipient mice. Lastly, we show that blockade of type I IFNR prevents antigenic tolerance induction against experimental allergic asthma. Overall, we unveil a new role for Opn in setting up a tolerogenic milieu boosting antigenic tolerance induction, thus leading to prevention of allergic airway inflammation. Our results provide insight for the future design of immunotherapies against allergic asthma.},
  author       = {Alissafi, Themis and Kourepini, Evangelia and Simoes, Davina CM and Paschalidis, Nikolaos and Aggelakopoulou, Maria and Sparwasser, Tim and Boon, Louis and Hammad, Hamida and Lambrecht, Bart and Panoutsakopoulou, Vily},
  issn         = {0022-1767},
  journal      = {JOURNAL OF IMMUNOLOGY},
  keywords     = {REGULATORY T-CELLS,PLASMACYTOID DENDRITIC CELLS,IN-VIVO,MEDIATED-IMMUNITY,TGF-BETA,ASTHMA,INFLAMMATION,RESPONSES,DIFFERENTIATION,EXPRESSION},
  language     = {eng},
  number       = {4},
  pages        = {1270--1282},
  title        = {Osteopontin promotes protective antigenic tolerance against experimental allergic airway disease},
  url          = {http://dx.doi.org/10.4049/jimmunol.1701345},
  volume       = {200},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: