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DPP4, the Middle East Respiratory Syndrome Coronavirus Receptor, is Upregulated in Lungs of Smokers and Chronic Obstructive Pulmonary Disease Patients

Leen Seys UGent, W. Widagdo, Fien Verhamme UGent, Alex Kleinjan, Wim Janssens, Guy Joos UGent, Ken Bracke UGent, Bart L. Haagmans and Guy Brusselle UGent (2018) CLINICAL INFECTIOUS DISEASES. 66(1). p.45-53
abstract
Background. Middle East respiratory syndrome coronavirus (MERS-CoV) causes pneumonia with a relatively high case fatality rate in humans. Smokers and chronic obstructive pulmonary disease (COPD) patients have been reported to be more susceptible to MERS-CoV infection. Here, we determined the expression of MERS-CoV receptor, dipeptidyl peptidase IV (DPP4), in lung tissues of smokers without airflow limitation and COPD patients in comparison to nonsmoking individuals (never-smokers). Methods. DPP4 expression was measured in lung tissue of lung resection specimens of never-smokers, smokers without airflow limitation, COPD GOLD stage II patients and in lung explants of end-stage COPD patients. Both control subjects and COPD patients were well phenotyped and age-matched. The mRNA expression was determined using qRT-PCR and protein expression was quantified using immunohistochemistry. Results. In smokers and subjects with COPD, both DPP4 mRNA and protein expression were significantly higher compared to never-smokers. Additionally, we found that both DPP4 mRNA and protein expression were inversely correlated with lung function and diffusing capacity parameters. Conclusions. We provide evidence that DPP4 is upregulated in the lungs of smokers and COPD patients, which could partially explain why these individuals are more susceptible to MERS-CoV infection. These data also highlight a possible role of DPP4 in COPD pathogenesis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DIPEPTIDYL PEPTIDASE 4, HEALTH-CARE WORKER, DENDRITIC CELLS, SAUDI-ARABIA, INFECTION, METHACHOLINE, EXPRESSION, CONTACTS, HUMANS, ASTHMA, Middle East respiratory syndrome coronavirus (MERS-CoV), dipeptidyl, peptidase 4 (DPP4), smoking chronic obstructive pulmonary disease (COPD)
journal title
CLINICAL INFECTIOUS DISEASES
Clin. Infect. Dis.
volume
66
issue
1
pages
9 pages
publisher
Oxford Univ Press Inc
place of publication
Cary
Web of Science type
Article
Web of Science id
000419648300011
ISSN
1058-4838
1537-6591
DOI
10.1093/cid/cix741
language
English
UGent publication?
yes
classification
U
copyright statement
Creative Commons Public Domain Dedication (CC0 1.0)
id
8554119
handle
http://hdl.handle.net/1854/LU-8554119
date created
2018-03-08 13:19:58
date last changed
2018-03-08 13:19:58
@article{8554119,
  abstract     = {Background. Middle East respiratory syndrome coronavirus (MERS-CoV) causes pneumonia with a relatively high case fatality rate in humans. Smokers and chronic obstructive pulmonary disease (COPD) patients have been reported to be more susceptible to MERS-CoV infection. Here, we determined the expression of MERS-CoV receptor, dipeptidyl peptidase IV (DPP4), in lung tissues of smokers without airflow limitation and COPD patients in comparison to nonsmoking individuals (never-smokers). Methods. DPP4 expression was measured in lung tissue of lung resection specimens of never-smokers, smokers without airflow limitation, COPD GOLD stage II patients and in lung explants of end-stage COPD patients. Both control subjects and COPD patients were well phenotyped and age-matched. The mRNA expression was determined using qRT-PCR and protein expression was quantified using immunohistochemistry. Results. In smokers and subjects with COPD, both DPP4 mRNA and protein expression were significantly higher compared to never-smokers. Additionally, we found that both DPP4 mRNA and protein expression were inversely correlated with lung function and diffusing capacity parameters. Conclusions. We provide evidence that DPP4 is upregulated in the lungs of smokers and COPD patients, which could partially explain why these individuals are more susceptible to MERS-CoV infection. These data also highlight a possible role of DPP4 in COPD pathogenesis.},
  author       = {Seys, Leen and Widagdo, W. and Verhamme, Fien and Kleinjan, Alex and Janssens, Wim and Joos, Guy and Bracke, Ken and Haagmans, Bart L. and Brusselle, Guy},
  issn         = {1058-4838},
  journal      = {CLINICAL INFECTIOUS DISEASES},
  keyword      = {DIPEPTIDYL PEPTIDASE 4,HEALTH-CARE WORKER,DENDRITIC CELLS,SAUDI-ARABIA,INFECTION,METHACHOLINE,EXPRESSION,CONTACTS,HUMANS,ASTHMA,Middle East respiratory syndrome coronavirus (MERS-CoV),dipeptidyl,peptidase 4 (DPP4),smoking chronic obstructive pulmonary disease (COPD)},
  language     = {eng},
  number       = {1},
  pages        = {45--53},
  publisher    = {Oxford Univ Press Inc},
  title        = {DPP4, the Middle East Respiratory Syndrome Coronavirus Receptor, is Upregulated in Lungs of Smokers and Chronic Obstructive Pulmonary Disease Patients},
  url          = {http://dx.doi.org/10.1093/cid/cix741},
  volume       = {66},
  year         = {2018},
}

Chicago
Seys, Leen, W. Widagdo, Fien Verhamme, Alex Kleinjan, Wim Janssens, Guy Joos, Ken Bracke, Bart L. Haagmans, and Guy Brusselle. 2018. “DPP4, the Middle East Respiratory Syndrome Coronavirus Receptor, Is Upregulated in Lungs of Smokers and Chronic Obstructive Pulmonary Disease Patients.” Clinical Infectious Diseases 66 (1): 45–53.
APA
Seys, L., Widagdo, W., Verhamme, F., Kleinjan, A., Janssens, W., Joos, G., Bracke, K., et al. (2018). DPP4, the Middle East Respiratory Syndrome Coronavirus Receptor, is Upregulated in Lungs of Smokers and Chronic Obstructive Pulmonary Disease Patients. CLINICAL INFECTIOUS DISEASES, 66(1), 45–53.
Vancouver
1.
Seys L, Widagdo W, Verhamme F, Kleinjan A, Janssens W, Joos G, et al. DPP4, the Middle East Respiratory Syndrome Coronavirus Receptor, is Upregulated in Lungs of Smokers and Chronic Obstructive Pulmonary Disease Patients. CLINICAL INFECTIOUS DISEASES. Cary: Oxford Univ Press Inc; 2018;66(1):45–53.
MLA
Seys, Leen, W. Widagdo, Fien Verhamme, et al. “DPP4, the Middle East Respiratory Syndrome Coronavirus Receptor, Is Upregulated in Lungs of Smokers and Chronic Obstructive Pulmonary Disease Patients.” CLINICAL INFECTIOUS DISEASES 66.1 (2018): 45–53. Print.