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Looking for phosphate-accumulating bacteria in activated sludge processes : a multidisciplinary approach

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Abstract
Over the past decades, an increasing need in renewable resources has progressively appeared. This trend concerns not only fossil fuels but also mineral resources. Wastewater and sewage sludge contain significant concentrations in phosphate and can be considered as a fertilizer source of the utmost importance. In wastewater treatment plants, the biological uptake of phosphate is performed by a specific microbiota: the phosphate-accumulating organisms. These microorganisms are recovered in sewage sludge. Here, we aimed to investigate the occurrence of phosphate accumulators in four wastewater treatment plants. A 16S metagenetic analysis identified the main bacterial phyla extracted from the aerobic treatment: alpha-Proteobacteria, beta-Proteobacteria, and Sphingobacteria. An enrichment stage was performed to stimulate the specific growth of phosphate-accumulating bacteria in an acetate medium. An analysis of metabolic activities of sulfur and phosphorus highlighted strong modifications related to phosphorus and much less distinguishable effects with sulfur. A solid acetate medium containing 5-Br-4-Cl-3-indolyl phosphate was used to select potential phosphate-accumulating bacteria from the enriched consortia. The positive strains have been found to belong in the genera Acinetobacter, Corynebacterium, and Pseudomonas. Finally, electron microscopy was applied to the strains and allowed to confirm the presence of polyphosphate granules. Some of these bacteria contained granules the size of which exceeded 100 nm.
Keywords
Phosphorus recovery, Polyphosphate, PAOs, Wastewater, Recycling, Microbial consortium, BIOLOGICAL PHOSPHORUS REMOVAL, WASTE-WATER, PSEUDOMONAS-AERUGINOSA, INORGANIC POLYPHOSPHATE, RECOVERY, SCALE, ACINETOBACTER, DESIGN, GUT

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MLA
Tarayre, Cédric, et al. “Looking for Phosphate-Accumulating Bacteria in Activated Sludge Processes : A Multidisciplinary Approach.” ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, vol. 24, no. 9, 2017, pp. 8017–32, doi:10.1007/s11356-017-8490-y.
APA
Tarayre, C., Charlier, R., Delepierre, A., Brognaux, A., Bauwens, J., Francis, F., … Delvigne, F. (2017). Looking for phosphate-accumulating bacteria in activated sludge processes : a multidisciplinary approach. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 24(9), 8017–8032. https://doi.org/10.1007/s11356-017-8490-y
Chicago author-date
Tarayre, Cédric, Raphaëlle Charlier, Anissa Delepierre, Alison Brognaux, Julien Bauwens, Frédéric Francis, Michaël Dermience, et al. 2017. “Looking for Phosphate-Accumulating Bacteria in Activated Sludge Processes : A Multidisciplinary Approach.” ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 24 (9): 8017–32. https://doi.org/10.1007/s11356-017-8490-y.
Chicago author-date (all authors)
Tarayre, Cédric, Raphaëlle Charlier, Anissa Delepierre, Alison Brognaux, Julien Bauwens, Frédéric Francis, Michaël Dermience, Georges Lognay, Bernard Taminiau, Georges Daube, Philippe Compère, Erik Meers, Evi Michels, and Frank Delvigne. 2017. “Looking for Phosphate-Accumulating Bacteria in Activated Sludge Processes : A Multidisciplinary Approach.” ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 24 (9): 8017–8032. doi:10.1007/s11356-017-8490-y.
Vancouver
1.
Tarayre C, Charlier R, Delepierre A, Brognaux A, Bauwens J, Francis F, et al. Looking for phosphate-accumulating bacteria in activated sludge processes : a multidisciplinary approach. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH. 2017;24(9):8017–32.
IEEE
[1]
C. Tarayre et al., “Looking for phosphate-accumulating bacteria in activated sludge processes : a multidisciplinary approach,” ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, vol. 24, no. 9, pp. 8017–8032, 2017.
@article{8554084,
  abstract     = {Over the past decades, an increasing need in renewable resources has progressively appeared. This trend concerns not only fossil fuels but also mineral resources. Wastewater and sewage sludge contain significant concentrations in phosphate and can be considered as a fertilizer source of the utmost importance. In wastewater treatment plants, the biological uptake of phosphate is performed by a specific microbiota: the phosphate-accumulating organisms. These microorganisms are recovered in sewage sludge. Here, we aimed to investigate the occurrence of phosphate accumulators in four wastewater treatment plants. A 16S metagenetic analysis identified the main bacterial phyla extracted from the aerobic treatment: alpha-Proteobacteria, beta-Proteobacteria, and Sphingobacteria. An enrichment stage was performed to stimulate the specific growth of phosphate-accumulating bacteria in an acetate medium. An analysis of metabolic activities of sulfur and phosphorus highlighted strong modifications related to phosphorus and much less distinguishable effects with sulfur. A solid acetate medium containing 5-Br-4-Cl-3-indolyl phosphate was used to select potential phosphate-accumulating bacteria from the enriched consortia. The positive strains have been found to belong in the genera Acinetobacter, Corynebacterium, and Pseudomonas. Finally, electron microscopy was applied to the strains and allowed to confirm the presence of polyphosphate granules. Some of these bacteria contained granules the size of which exceeded 100 nm.},
  author       = {Tarayre, Cédric and Charlier, Raphaëlle and Delepierre, Anissa and Brognaux, Alison and Bauwens, Julien and Francis, Frédéric and Dermience, Michaël and Lognay, Georges and Taminiau, Bernard and Daube, Georges and Compère, Philippe and Meers, Erik and Michels, Evi and Delvigne, Frank},
  issn         = {0944-1344},
  journal      = {ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH},
  keywords     = {Phosphorus recovery,Polyphosphate,PAOs,Wastewater,Recycling,Microbial consortium,BIOLOGICAL PHOSPHORUS REMOVAL,WASTE-WATER,PSEUDOMONAS-AERUGINOSA,INORGANIC POLYPHOSPHATE,RECOVERY,SCALE,ACINETOBACTER,DESIGN,GUT},
  language     = {eng},
  number       = {9},
  pages        = {8017--8032},
  title        = {Looking for phosphate-accumulating bacteria in activated sludge processes : a multidisciplinary approach},
  url          = {http://dx.doi.org/10.1007/s11356-017-8490-y},
  volume       = {24},
  year         = {2017},
}

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