Transgenerational DNA methylation changes in Daphnia magna exposed to chronic γ irradiation
- Author
- Marie Trijau, Jana Asselman (UGent) , Olivier Armant, Christelle Adam-Guillermin, Karel De Schamphelaere (UGent) and Frédéric Alonzo
- Organization
- Abstract
- Our aim was to investigate epigenetic changes in Daphnia magna after a 25-day chronic external gamma irradiation (generation F0 exposed to 6.5 mu Gy.h(-1) or 41.3 mGy.h(-1)) and their potential inheritance by subsequent recovering generations, namely, F2 (exposed as germline cells in F1 embryos) and F3 (the first truly unexposed generation). Effects on survival, growth, and reproduction were observed and DNA was extracted for whole-genome bisulfite sequencing in all generations. Results showed effects on reproduction in F0 but no effect in the subsequent generations F1, F2, and F3. In contrast, we observed significant methylation changes at specific CpG positions in every generation independent of dose rate, with a majority of hypomethylation. Some of these changes were shared between dose rates and between generations. Associated gene functions included gene families and genes that were previously shown to play roles during exposure to ionizing radiation. Common methylation changes detected between generations F2 and F3 clearly showed that epigenetic modifications can be transmitted to unexposed generations, most likely through the germline, with potential implications for environmental risk.
- Keywords
- EISENIA-FOETIDA OLIGOCHAETA, X-RAY-IRRADIATION, IONIZING-RADIATION, GENOME STABILITY, GENE-EXPRESSION, INDUCIBLE HEAT-SHOCK-PROTEIN-70, RADIOADAPTIVE RESPONSE, EPIGENETIC INHERITANCE, SERINE PROTEASES, MEDAKA FISH
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8553733
- MLA
- Trijau, Marie, et al. “Transgenerational DNA Methylation Changes in Daphnia Magna Exposed to Chronic γ Irradiation.” ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol. 52, no. 7, 2018, pp. 4331–39, doi:10.1021/acs.est.7b05695.
- APA
- Trijau, M., Asselman, J., Armant, O., Adam-Guillermin, C., De Schamphelaere, K., & Alonzo, F. (2018). Transgenerational DNA methylation changes in Daphnia magna exposed to chronic γ irradiation. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 52(7), 4331–4339. https://doi.org/10.1021/acs.est.7b05695
- Chicago author-date
- Trijau, Marie, Jana Asselman, Olivier Armant, Christelle Adam-Guillermin, Karel De Schamphelaere, and Frédéric Alonzo. 2018. “Transgenerational DNA Methylation Changes in Daphnia Magna Exposed to Chronic γ Irradiation.” ENVIRONMENTAL SCIENCE & TECHNOLOGY 52 (7): 4331–39. https://doi.org/10.1021/acs.est.7b05695.
- Chicago author-date (all authors)
- Trijau, Marie, Jana Asselman, Olivier Armant, Christelle Adam-Guillermin, Karel De Schamphelaere, and Frédéric Alonzo. 2018. “Transgenerational DNA Methylation Changes in Daphnia Magna Exposed to Chronic γ Irradiation.” ENVIRONMENTAL SCIENCE & TECHNOLOGY 52 (7): 4331–4339. doi:10.1021/acs.est.7b05695.
- Vancouver
- 1.Trijau M, Asselman J, Armant O, Adam-Guillermin C, De Schamphelaere K, Alonzo F. Transgenerational DNA methylation changes in Daphnia magna exposed to chronic γ irradiation. ENVIRONMENTAL SCIENCE & TECHNOLOGY. 2018;52(7):4331–9.
- IEEE
- [1]M. Trijau, J. Asselman, O. Armant, C. Adam-Guillermin, K. De Schamphelaere, and F. Alonzo, “Transgenerational DNA methylation changes in Daphnia magna exposed to chronic γ irradiation,” ENVIRONMENTAL SCIENCE & TECHNOLOGY, vol. 52, no. 7, pp. 4331–4339, 2018.
@article{8553733,
abstract = {{Our aim was to investigate epigenetic changes in Daphnia magna after a 25-day chronic external gamma irradiation (generation F0 exposed to 6.5 mu Gy.h(-1) or 41.3 mGy.h(-1)) and their potential inheritance by subsequent recovering generations, namely, F2 (exposed as germline cells in F1 embryos) and F3 (the first truly unexposed generation). Effects on survival, growth, and reproduction were observed and DNA was extracted for whole-genome bisulfite sequencing in all generations. Results showed effects on reproduction in F0 but no effect in the subsequent generations F1, F2, and F3. In contrast, we observed significant methylation changes at specific CpG positions in every generation independent of dose rate, with a majority of hypomethylation. Some of these changes were shared between dose rates and between generations. Associated gene functions included gene families and genes that were previously shown to play roles during exposure to ionizing radiation. Common methylation changes detected between generations F2 and F3 clearly showed that epigenetic modifications can be transmitted to unexposed generations, most likely through the germline, with potential implications for environmental risk.}},
author = {{Trijau, Marie and Asselman, Jana and Armant, Olivier and Adam-Guillermin, Christelle and De Schamphelaere, Karel and Alonzo, Frédéric}},
issn = {{0013-936X}},
journal = {{ENVIRONMENTAL SCIENCE & TECHNOLOGY}},
keywords = {{EISENIA-FOETIDA OLIGOCHAETA,X-RAY-IRRADIATION,IONIZING-RADIATION,GENOME STABILITY,GENE-EXPRESSION,INDUCIBLE HEAT-SHOCK-PROTEIN-70,RADIOADAPTIVE RESPONSE,EPIGENETIC INHERITANCE,SERINE PROTEASES,MEDAKA FISH}},
language = {{eng}},
number = {{7}},
pages = {{4331--4339}},
title = {{Transgenerational DNA methylation changes in Daphnia magna exposed to chronic γ irradiation}},
url = {{http://doi.org/10.1021/acs.est.7b05695}},
volume = {{52}},
year = {{2018}},
}
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