Advanced search
1 file | 3.82 MB Add to list

Fertility preservation for trans men : frozen-thawed in vitro matured oocytes collected at the time of ovarian tissue processing exhibit normal meiotic spindles

Sylvie Lierman (UGent) , Kelly Tilleman (UGent) , Kevin Braeckmans (UGent) , Karen Peynshaert (UGent) , Steven Weyers (UGent) , Guy T'Sjoen (UGent) and Petra De Sutter (UGent)
Author
Organization
Abstract
Purposes: At the moment of sex reassignment surgery (SRS), the ovarian tissue is sometimes cryopreserved as fertility preservation option for female-to-male trans men, also called trans men. During this preparation, cumulus-oocyte-complexes (COCs) can be found and in vitro matured. It is not known if these oocytes are developmentally competent. In order to use these oocytes for fertility preservation and subsequent fertilization, a normal spindle structure before and after vitrification is necessary. Methods: A total of 680 COCs were collected from trans men (n = 16) at the time of SRS and after testosterone treatment. The COCs were subjected to in vitro maturation and those that reached the metaphase II stage (MII) were collected and split into two groups; group 1 was immediately fixed for spindle staining and group 2 was first vitrified and warmed followed by spindle staining. Statistical analysis was performed by Fisher's exact test. Results: After 48 h in vitro maturation, 38.1% of COCs were at MII stage. Those oocytes were split in two groups: (1) 126 MII oocytes in the noncryopreservation group and (2) 133 MII oocytes underwent cryopreservation through vitrification. The oocyte survival rate, after 2 h warming, was 67.7%. Both the noncryopreserved and the vitrified group showed comparable results concerning normal spindle structure and chromosomes alignment, 85.7% vs. 92.2% (P = 0.27). Conclusions: Spindle structure analysis and chromosomal alignment after vitrification seem normal in in vitro matured COCs collected during the tissue processing of ovaries in trans men at the time of SRS. The MII oocytes do not seem to be morphologically affected by prolonged testosterone treatment.
Keywords
CANCER-PATIENTS, VITRIFICATION, CRYOPRESERVATION, MATURATION, FOLLICLES, CULTURE, IVM, Human ovarian medulla tissue, Metaphase II oocytes, Vitrification, Spindle structure, In vitro maturation

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 3.82 MB

Citation

Please use this url to cite or link to this publication:

MLA
Lierman, Sylvie et al. “Fertility Preservation for Trans Men : Frozen-thawed in Vitro Matured Oocytes Collected at the Time of Ovarian Tissue Processing Exhibit Normal Meiotic Spindles.” JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 34.11 (2017): 1449–1456. Print.
APA
Lierman, S., Tilleman, K., Braeckmans, K., Peynshaert, K., Weyers, S., T’Sjoen, G., & De Sutter, P. (2017). Fertility preservation for trans men : frozen-thawed in vitro matured oocytes collected at the time of ovarian tissue processing exhibit normal meiotic spindles. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 34(11), 1449–1456.
Chicago author-date
Lierman, Sylvie, Kelly Tilleman, Kevin Braeckmans, Karen Peynshaert, Steven Weyers, Guy T’Sjoen, and Petra De Sutter. 2017. “Fertility Preservation for Trans Men : Frozen-thawed in Vitro Matured Oocytes Collected at the Time of Ovarian Tissue Processing Exhibit Normal Meiotic Spindles.” Journal of Assisted Reproduction and Genetics 34 (11): 1449–1456.
Chicago author-date (all authors)
Lierman, Sylvie, Kelly Tilleman, Kevin Braeckmans, Karen Peynshaert, Steven Weyers, Guy T’Sjoen, and Petra De Sutter. 2017. “Fertility Preservation for Trans Men : Frozen-thawed in Vitro Matured Oocytes Collected at the Time of Ovarian Tissue Processing Exhibit Normal Meiotic Spindles.” Journal of Assisted Reproduction and Genetics 34 (11): 1449–1456.
Vancouver
1.
Lierman S, Tilleman K, Braeckmans K, Peynshaert K, Weyers S, T’Sjoen G, et al. Fertility preservation for trans men : frozen-thawed in vitro matured oocytes collected at the time of ovarian tissue processing exhibit normal meiotic spindles. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS. 2017;34(11):1449–56.
IEEE
[1]
S. Lierman et al., “Fertility preservation for trans men : frozen-thawed in vitro matured oocytes collected at the time of ovarian tissue processing exhibit normal meiotic spindles,” JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol. 34, no. 11, pp. 1449–1456, 2017.
@article{8553546,
  abstract     = {Purposes: At the moment of sex reassignment surgery (SRS), the ovarian tissue is sometimes cryopreserved as fertility preservation option for female-to-male trans men, also called trans men. During this preparation, cumulus-oocyte-complexes (COCs) can be found and in vitro matured. It is not known if these oocytes are developmentally competent. In order to use these oocytes for fertility preservation and subsequent fertilization, a normal spindle structure before and after vitrification is necessary. 
Methods: A total of 680 COCs were collected from trans men (n = 16) at the time of SRS and after testosterone treatment. The COCs were subjected to in vitro maturation and those that reached the metaphase II stage (MII) were collected and split into two groups; group 1 was immediately fixed for spindle staining and group 2 was first vitrified and warmed followed by spindle staining. Statistical analysis was performed by Fisher's exact test. 
Results: After 48 h in vitro maturation, 38.1% of COCs were at MII stage. Those oocytes were split in two groups: (1) 126 MII oocytes in the noncryopreservation group and (2) 133 MII oocytes underwent cryopreservation through vitrification. The oocyte survival rate, after 2 h warming, was 67.7%. Both the noncryopreserved and the vitrified group showed comparable results concerning normal spindle structure and chromosomes alignment, 85.7% vs. 92.2% (P = 0.27). 
Conclusions: Spindle structure analysis and chromosomal alignment after vitrification seem normal in in vitro matured COCs collected during the tissue processing of ovaries in trans men at the time of SRS. The MII oocytes do not seem to be morphologically affected by prolonged testosterone treatment.},
  author       = {Lierman, Sylvie and Tilleman, Kelly and Braeckmans, Kevin and Peynshaert, Karen and Weyers, Steven and T'Sjoen, Guy and De Sutter, Petra},
  issn         = {1058-0468},
  journal      = {JOURNAL OF ASSISTED REPRODUCTION AND GENETICS},
  keywords     = {CANCER-PATIENTS,VITRIFICATION,CRYOPRESERVATION,MATURATION,FOLLICLES,CULTURE,IVM,Human ovarian medulla tissue,Metaphase II oocytes,Vitrification,Spindle structure,In vitro maturation},
  language     = {eng},
  number       = {11},
  pages        = {1449--1456},
  title        = {Fertility preservation for trans men : frozen-thawed in vitro matured oocytes collected at the time of ovarian tissue processing exhibit normal meiotic spindles},
  url          = {http://dx.doi.org/10.1007/s10815-017-0976-5},
  volume       = {34},
  year         = {2017},
}

Altmetric
View in Altmetric
Web of Science
Times cited: