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Arabinoxylo-oligosaccharides and inulin impact inter-individual variation on microbial metabolism and composition, which immunomodulates human cells

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Abstract
Fecal batch fermentations coupled to cocultures of epithelial cells and macrophages were used to compare how arabinoxylo-oligosaccharides (AXOS) and inulin modulate gut microbial activity and composition of three different human donors and subsequently the epithelial permeability and immune response. Both inulin and AXOS decreased the pH during incubation (-1.5 pH units), leading to increased productions of acetate, propionate, and butyrate. Differences in terms of metabolites production could be linked to specific microbial alterations at genus level upon inulin/AXOS supplementation (i.e., Bifidobacterium, Bacteroides, Prevotella and unclassified Erysipelotrichaceae), as shown by 16S-targeted Illumina sequencing. Both products stimulated gut barrier and immune function with increases in TEER, NF-KB, IL-10, and IL-6. Ingredients with different structures selectively modulate the microbiota of a specific donor leading to differential changes at metabolic level. The extent of this effect is donor specific and is linked to a final specific modulation of the host's immune system.
Keywords
arabinoxylan, fructan, gut microbiota, Caco-2 cells, In vitro, CHAIN FATTY-ACIDS, HUMAN GUT, GASTROINTESTINAL-TRACT, INTESTINAL MICROBIOTA, FECAL MICROFLORA, ADIPOSE-TISSUE, PROPIONIC-ACID, RIBOSOMAL-RNA, HUMAN FECES, FERMENTATION

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Citation

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MLA
Van den Abbeele, Pieter, Bernard Taminiau, Iris Pinheiro, et al. “Arabinoxylo-oligosaccharides and Inulin Impact Inter-individual Variation on Microbial Metabolism and Composition, Which Immunomodulates Human Cells.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 66.5 (2018): 1121–1130. Print.
APA
Van den Abbeele, P., Taminiau, B., Pinheiro, I., Duysburgh, C., Jacobs, H., Pijls, L., & Marzorati, M. (2018). Arabinoxylo-oligosaccharides and inulin impact inter-individual variation on microbial metabolism and composition, which immunomodulates human cells. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 66(5), 1121–1130.
Chicago author-date
Van den Abbeele, Pieter, Bernard Taminiau, Iris Pinheiro, Cindy Duysburgh, Heidi Jacobs, Loek Pijls, and Massimo Marzorati. 2018. “Arabinoxylo-oligosaccharides and Inulin Impact Inter-individual Variation on Microbial Metabolism and Composition, Which Immunomodulates Human Cells.” Journal of Agricultural and Food Chemistry 66 (5): 1121–1130.
Chicago author-date (all authors)
Van den Abbeele, Pieter, Bernard Taminiau, Iris Pinheiro, Cindy Duysburgh, Heidi Jacobs, Loek Pijls, and Massimo Marzorati. 2018. “Arabinoxylo-oligosaccharides and Inulin Impact Inter-individual Variation on Microbial Metabolism and Composition, Which Immunomodulates Human Cells.” Journal of Agricultural and Food Chemistry 66 (5): 1121–1130.
Vancouver
1.
Van den Abbeele P, Taminiau B, Pinheiro I, Duysburgh C, Jacobs H, Pijls L, et al. Arabinoxylo-oligosaccharides and inulin impact inter-individual variation on microbial metabolism and composition, which immunomodulates human cells. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2018;66(5):1121–30.
IEEE
[1]
P. Van den Abbeele et al., “Arabinoxylo-oligosaccharides and inulin impact inter-individual variation on microbial metabolism and composition, which immunomodulates human cells,” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 66, no. 5, pp. 1121–1130, 2018.
@article{8553517,
  abstract     = {Fecal batch fermentations coupled to cocultures of epithelial cells and macrophages were used to compare how arabinoxylo-oligosaccharides (AXOS) and inulin modulate gut microbial activity and composition of three different human donors and subsequently the epithelial permeability and immune response. Both inulin and AXOS decreased the pH during incubation (-1.5 pH units), leading to increased productions of acetate, propionate, and butyrate. Differences in terms of metabolites production could be linked to specific microbial alterations at genus level upon inulin/AXOS supplementation (i.e., Bifidobacterium, Bacteroides, Prevotella and unclassified Erysipelotrichaceae), as shown by 16S-targeted Illumina sequencing. Both products stimulated gut barrier and immune function with increases in TEER, NF-KB, IL-10, and IL-6. Ingredients with different structures selectively modulate the microbiota of a specific donor leading to differential changes at metabolic level. The extent of this effect is donor specific and is linked to a final specific modulation of the host's immune system.},
  author       = {Van den Abbeele, Pieter and Taminiau, Bernard and Pinheiro, Iris and Duysburgh, Cindy and Jacobs, Heidi and Pijls, Loek and Marzorati, Massimo},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keywords     = {arabinoxylan,fructan,gut microbiota,Caco-2 cells,In vitro,CHAIN FATTY-ACIDS,HUMAN GUT,GASTROINTESTINAL-TRACT,INTESTINAL MICROBIOTA,FECAL MICROFLORA,ADIPOSE-TISSUE,PROPIONIC-ACID,RIBOSOMAL-RNA,HUMAN FECES,FERMENTATION},
  language     = {eng},
  number       = {5},
  pages        = {1121--1130},
  title        = {Arabinoxylo-oligosaccharides and inulin impact inter-individual variation on microbial metabolism and composition, which immunomodulates human cells},
  url          = {http://dx.doi.org/10.1021/acs.jafc.7b04611},
  volume       = {66},
  year         = {2018},
}

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