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Initial evenness determines diversity and cell density dynamics in synthetic microbial ecosystems

Elham Ehsani, Emma Hernandez Sanabria (UGent) , Frederiek-Maarten Kerckhof (UGent) , Ruben Props (UGent) , Ramiro Vilchez Vargas (UGent) , Marius Vital, Dietmar H Pieper and Nico Boon (UGent)
(2018) SCIENTIFIC REPORTS. 8.
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Abstract
The effect of initial evenness on the temporal trajectory of synthetic communities in comprehensive, low-volume microcosm studies remains unknown. We used flow cytometric fingerprinting and 16S rRNA gene amplicon sequencing to assess the impact of time on community structure in one hundred synthetic ecosystems of fixed richness but varying initial evenness. Both methodologies uncovered a similar reduction in diversity within synthetic communities of medium and high initial evenness classes. However, the results of amplicon sequencing showed that there were no significant differences between and within the communities in all evenness groups at the end of the experiment. Nevertheless, initial evenness significantly impacted the cell density of the community after five medium transfers. Highly even communities retained the highest cell densities at the end of the experiment. The relative abundances of individual species could be associated to particular evenness groups, suggesting that their presence was dependent on the initial evenness of the synthetic community. Our results reveal that using synthetic communities for testing ecological hypotheses requires prior assessment of initial evenness, as it impacts temporal dynamics.
Keywords
RIBOSOMAL-RNA GENE, FLOW-CYTOMETRY, DRINKING-WATER, COMMUNITY STRUCTURE, BACTERIAL, ECOLOGY, SYSTEM, FUNCTIONALITY, COOPERATION, COMPETITION

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MLA
Ehsani, Elham, et al. “Initial Evenness Determines Diversity and Cell Density Dynamics in Synthetic Microbial Ecosystems.” SCIENTIFIC REPORTS, vol. 8, 2018, doi:10.1038/s41598-017-18668-1.
APA
Ehsani, E., Hernandez Sanabria, E., Kerckhof, F.-M., Props, R., Vilchez Vargas, R., Vital, M., … Boon, N. (2018). Initial evenness determines diversity and cell density dynamics in synthetic microbial ecosystems. SCIENTIFIC REPORTS, 8. https://doi.org/10.1038/s41598-017-18668-1
Chicago author-date
Ehsani, Elham, Emma Hernandez Sanabria, Frederiek-Maarten Kerckhof, Ruben Props, Ramiro Vilchez Vargas, Marius Vital, Dietmar H Pieper, and Nico Boon. 2018. “Initial Evenness Determines Diversity and Cell Density Dynamics in Synthetic Microbial Ecosystems.” SCIENTIFIC REPORTS 8. https://doi.org/10.1038/s41598-017-18668-1.
Chicago author-date (all authors)
Ehsani, Elham, Emma Hernandez Sanabria, Frederiek-Maarten Kerckhof, Ruben Props, Ramiro Vilchez Vargas, Marius Vital, Dietmar H Pieper, and Nico Boon. 2018. “Initial Evenness Determines Diversity and Cell Density Dynamics in Synthetic Microbial Ecosystems.” SCIENTIFIC REPORTS 8. doi:10.1038/s41598-017-18668-1.
Vancouver
1.
Ehsani E, Hernandez Sanabria E, Kerckhof F-M, Props R, Vilchez Vargas R, Vital M, et al. Initial evenness determines diversity and cell density dynamics in synthetic microbial ecosystems. SCIENTIFIC REPORTS. 2018;8.
IEEE
[1]
E. Ehsani et al., “Initial evenness determines diversity and cell density dynamics in synthetic microbial ecosystems,” SCIENTIFIC REPORTS, vol. 8, 2018.
@article{8553304,
  abstract     = {{The effect of initial evenness on the temporal trajectory of synthetic communities in comprehensive, low-volume microcosm studies remains unknown. We used flow cytometric fingerprinting and 16S rRNA gene amplicon sequencing to assess the impact of time on community structure in one hundred synthetic ecosystems of fixed richness but varying initial evenness. Both methodologies uncovered a similar reduction in diversity within synthetic communities of medium and high initial evenness classes. However, the results of amplicon sequencing showed that there were no significant differences between and within the communities in all evenness groups at the end of the experiment. Nevertheless, initial evenness significantly impacted the cell density of the community after five medium transfers. Highly even communities retained the highest cell densities at the end of the experiment. The relative abundances of individual species could be associated to particular evenness groups, suggesting that their presence was dependent on the initial evenness of the synthetic community. Our results reveal that using synthetic communities for testing ecological hypotheses requires prior assessment of initial evenness, as it impacts temporal dynamics.}},
  articleno    = {{340}},
  author       = {{Ehsani, Elham and Hernandez Sanabria, Emma and Kerckhof, Frederiek-Maarten and Props, Ruben and Vilchez Vargas, Ramiro and Vital, Marius and Pieper, Dietmar H and Boon, Nico}},
  issn         = {{2045-2322}},
  journal      = {{SCIENTIFIC REPORTS}},
  keywords     = {{RIBOSOMAL-RNA GENE,FLOW-CYTOMETRY,DRINKING-WATER,COMMUNITY STRUCTURE,BACTERIAL,ECOLOGY,SYSTEM,FUNCTIONALITY,COOPERATION,COMPETITION}},
  language     = {{eng}},
  pages        = {{9}},
  title        = {{Initial evenness determines diversity and cell density dynamics in synthetic microbial ecosystems}},
  url          = {{http://doi.org/10.1038/s41598-017-18668-1}},
  volume       = {{8}},
  year         = {{2018}},
}
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