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Genetic and pathogenic characterization of a Russian subtype 2 PRRSV-1 isolate

Anton G Yuzhakov, Sergei A Raev, Andrei N Skrylev, Alexander M Mishin, Tatiana V Grebennikova, Oleg A Verkhovsky, Alexei D Zaberezhny, Ivan Trus, Hans Nauwynck UGent and Taras I Aliper (2017) VETERINARY MICROBIOLOGY. 211. p.22-28
abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) causes reproductive failure and respiratory problems. Data about the virulence and pathogenicity of subtype 2 PRRSV-1 strains are limited. The main purposes of this investigation were to characterize the full genome sequence of the subtype 2 PRRSV-1 WestSib13 strain and to compare the pathogenicity with that of the subtype 1 PRRSV-1 Lelystad strain. Comparison of the whole genome sequence of the WestSib13 strain with that of PRRSV-1 prototype strains revealed a 76.2% (subtype 1 Lelystad virus) and 79.0% (subtype 3 Lena virus) identity, respectively The virulence and pathogenicity of the European subtype 2 PRRSV strain WestSib13 and the European subtype 1 PRRSV strain Lelystad were compared in 3-week-old piglets upon inoculation of 105HTC/SUPTAG'FORTITLEHTC_RETAIN(4) TCID50 of virus. Non-infected animals (control group) as well as animals infected with the Lelystad strain were clinically healthy until 14 days post challenge. In contrast, animals infected with the WestSib13 strain demonstrated dyspnea starting at 3 days post-inoculation (dpi). All piglets in this group died between 5 and 8 dpi. During that period, fever was not observed in WestSib13-infected animals. Viremia was detected in animals from both infected groups starting from 2 dpi. Viral loads in serum and lungs upon euthanasia were significantly higher (3 log(10)) in the WestSib13-infected than in the LV-infected animals. Taken together, this study provides the full genome sequence and the unusual virological and clinical outcome (high level viremia without fever) of the novel WestSib13 strain.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PRRSV, Subtype 2, Genome, Virulence, Pathogenicity, Mortality, Fever, RESPIRATORY SYNDROME VIRUS, DISEASE, EUROPE, DEFINITION, STRAINS, VACCINE, PIGS
journal title
VETERINARY MICROBIOLOGY
Vet. Microbiol.
volume
211
pages
22 - 28
Web of Science type
Article
Web of Science id
000416190200004
ISSN
0378-1135
DOI
10.1016/j.vetmic.2017.09.017
language
English
UGent publication?
yes
classification
A1
additional info
the first two authors contributed equally to this work
copyright statement
I have transferred the copyright for this publication to the publisher
id
8552140
handle
http://hdl.handle.net/1854/LU-8552140
date created
2018-02-27 12:15:10
date last changed
2018-03-12 15:49:24
@article{8552140,
  abstract     = {Porcine reproductive and respiratory syndrome virus (PRRSV) causes reproductive failure and respiratory problems. Data about the virulence and pathogenicity of subtype 2 PRRSV-1 strains are limited. The main purposes of this investigation were to characterize the full genome sequence of the subtype 2 PRRSV-1 WestSib13 strain and to compare the pathogenicity with that of the subtype 1 PRRSV-1 Lelystad strain. Comparison of the whole genome sequence of the WestSib13 strain with that of PRRSV-1 prototype strains revealed a 76.2\% (subtype 1 Lelystad virus) and 79.0\% (subtype 3 Lena virus) identity, respectively 
The virulence and pathogenicity of the European subtype 2 PRRSV strain WestSib13 and the European subtype 1 PRRSV strain Lelystad were compared in 3-week-old piglets upon inoculation of 105HTC/SUPTAG'FORTITLEHTC\_RETAIN(4) TCID50 of virus. Non-infected animals (control group) as well as animals infected with the Lelystad strain were clinically healthy until 14 days post challenge. In contrast, animals infected with the WestSib13 strain demonstrated dyspnea starting at 3 days post-inoculation (dpi). All piglets in this group died between 5 and 8 dpi. During that period, fever was not observed in WestSib13-infected animals. Viremia was detected in animals from both infected groups starting from 2 dpi. Viral loads in serum and lungs upon euthanasia were significantly higher (3 log(10)) in the WestSib13-infected than in the LV-infected animals. 
Taken together, this study provides the full genome sequence and the unusual virological and clinical outcome (high level viremia without fever) of the novel WestSib13 strain.},
  author       = {Yuzhakov, Anton G and Raev, Sergei A and Skrylev, Andrei N and Mishin, Alexander M and Grebennikova, Tatiana V and Verkhovsky, Oleg A and Zaberezhny, Alexei D and Trus, Ivan and Nauwynck, Hans and Aliper, Taras I},
  issn         = {0378-1135},
  journal      = {VETERINARY MICROBIOLOGY},
  keyword      = {PRRSV,Subtype 2,Genome,Virulence,Pathogenicity,Mortality,Fever,RESPIRATORY SYNDROME VIRUS,DISEASE,EUROPE,DEFINITION,STRAINS,VACCINE,PIGS},
  language     = {eng},
  pages        = {22--28},
  title        = {Genetic and pathogenic characterization of a Russian subtype 2 PRRSV-1 isolate},
  url          = {http://dx.doi.org/10.1016/j.vetmic.2017.09.017},
  volume       = {211},
  year         = {2017},
}

Chicago
Yuzhakov, Anton G, Sergei A Raev, Andrei N Skrylev, Alexander M Mishin, Tatiana V Grebennikova, Oleg A Verkhovsky, Alexei D Zaberezhny, Ivan Trus, Hans Nauwynck, and Taras I Aliper. 2017. “Genetic and Pathogenic Characterization of a Russian Subtype 2 PRRSV-1 Isolate.” Veterinary Microbiology 211: 22–28.
APA
Yuzhakov, A. G., Raev, S. A., Skrylev, A. N., Mishin, A. M., Grebennikova, T. V., Verkhovsky, O. A., Zaberezhny, A. D., et al. (2017). Genetic and pathogenic characterization of a Russian subtype 2 PRRSV-1 isolate. VETERINARY MICROBIOLOGY, 211, 22–28.
Vancouver
1.
Yuzhakov AG, Raev SA, Skrylev AN, Mishin AM, Grebennikova TV, Verkhovsky OA, et al. Genetic and pathogenic characterization of a Russian subtype 2 PRRSV-1 isolate. VETERINARY MICROBIOLOGY. 2017;211:22–8.
MLA
Yuzhakov, Anton G, Sergei A Raev, Andrei N Skrylev, et al. “Genetic and Pathogenic Characterization of a Russian Subtype 2 PRRSV-1 Isolate.” VETERINARY MICROBIOLOGY 211 (2017): 22–28. Print.