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Experimental infection model for vibriosis in Dover sole (Solea solea) larvae as an aid in studying its pathogenesis and alternative treatments

Evelien De Swaef (UGent) , Maaike Vercauteren (UGent) , Luc Duchateau (UGent) , Freddy Haesebrouck (UGent) and Annemie Decostere (UGent)
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Abstract
Severe economic losses due to diseases in marine larviculture may be linked to vibriosis. To better understand the pathogenesis of vibriosis and evaluate new ways to prevent and combat this important disease, there is a great need for reliable and reproducible experimental infection models. The present study aimed at developing a challenge model for vibriosis in Dover sole larvae and testing its applicability to study the effect of the probiotic treatment. For that purpose, larvae were challenged at 10 days post hatching with Vibrio anguillarum WT, V. anguillarum HI610 or V. harveyi WT. Following administration of V. anguillarum WT via immersion at 1 × 107 colony forming units/mL, a larval mortality of 50% was observed at 17 days post-inoculation. In a next step, the probiotic potential of 371 isolates retrieved from Dover sole was assessed by screening for their inhibitory effects against Vibrio spp. and absence of haemolytic activity. One remaining isolate (V. proteolyticus) and V. lentus, known for its protective characteristics in seabass larvae, were further tested in vivo by means of the pinpointed experimental infection model. Neither isolate provided via the water or feed proved to be protective for the Dover sole larvae against challenge with V. anguillarum WT. This developed challenge model constitutes a firm basis to expedite basic and applied research regarding the pathogenesis and treatment of vibriosis as well as for studying the impact of (a)biotic components on larval health.
Keywords
TURBOT SCOPHTHALMUS-MAXIMUS, DICENTRARCHUS-LABRAX L., LACTIC-ACID BACTERIA, COD GADUS-MORHUA, YOLK-SAC LARVAE, LISTONELLA-ANGUILLARUM, PROBIOTIC BACTERIA, SENEGALENSIS KAUP, CHALLENGE TEST, FOOD-CHAIN

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Citation

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MLA
De Swaef, Evelien, Maaike Vercauteren, Luc Duchateau, et al. “Experimental Infection Model for Vibriosis in Dover Sole (Solea Solea) Larvae as an Aid in Studying Its Pathogenesis and Alternative Treatments.” VETERINARY RESEARCH 49 (2018): n. pag. Print.
APA
De Swaef, E., Vercauteren, M., Duchateau, L., Haesebrouck, F., & Decostere, A. (2018). Experimental infection model for vibriosis in Dover sole (Solea solea) larvae as an aid in studying its pathogenesis and alternative treatments. VETERINARY RESEARCH, 49.
Chicago author-date
De Swaef, Evelien, Maaike Vercauteren, Luc Duchateau, Freddy Haesebrouck, and Annemie Decostere. 2018. “Experimental Infection Model for Vibriosis in Dover Sole (Solea Solea) Larvae as an Aid in Studying Its Pathogenesis and Alternative Treatments.” Veterinary Research 49.
Chicago author-date (all authors)
De Swaef, Evelien, Maaike Vercauteren, Luc Duchateau, Freddy Haesebrouck, and Annemie Decostere. 2018. “Experimental Infection Model for Vibriosis in Dover Sole (Solea Solea) Larvae as an Aid in Studying Its Pathogenesis and Alternative Treatments.” Veterinary Research 49.
Vancouver
1.
De Swaef E, Vercauteren M, Duchateau L, Haesebrouck F, Decostere A. Experimental infection model for vibriosis in Dover sole (Solea solea) larvae as an aid in studying its pathogenesis and alternative treatments. VETERINARY RESEARCH. 2018;49.
IEEE
[1]
E. De Swaef, M. Vercauteren, L. Duchateau, F. Haesebrouck, and A. Decostere, “Experimental infection model for vibriosis in Dover sole (Solea solea) larvae as an aid in studying its pathogenesis and alternative treatments,” VETERINARY RESEARCH, vol. 49, 2018.
@article{8552080,
  abstract     = {Severe economic losses due to diseases in marine larviculture may be linked to vibriosis. To better understand the pathogenesis of vibriosis and evaluate new ways to prevent and combat this important disease, there is a great need for reliable and reproducible experimental infection models. The present study aimed at developing a challenge model for vibriosis in Dover sole larvae and testing its applicability to study the effect of the probiotic treatment. For that purpose, larvae were challenged at 10 days post hatching with Vibrio anguillarum WT, V. anguillarum HI610 or V. harveyi WT. Following administration of V. anguillarum WT via immersion at 1 × 107 colony forming units/mL, a larval mortality of 50% was observed at 17 days post-inoculation. In a next step, the probiotic potential of 371 isolates retrieved from Dover sole was assessed by screening for their inhibitory effects against Vibrio spp. and absence of haemolytic activity. One remaining isolate (V. proteolyticus) and V. lentus, known for its protective characteristics in seabass larvae, were further tested in vivo by means of the pinpointed experimental infection model. Neither isolate provided via the water or feed proved to be protective for the Dover sole larvae against challenge with V. anguillarum WT. This developed challenge model constitutes a firm basis to expedite basic and applied research regarding the pathogenesis and treatment of vibriosis as well as for studying the impact of (a)biotic components on larval health.},
  articleno    = {24},
  author       = {De Swaef, Evelien and Vercauteren, Maaike and Duchateau, Luc and Haesebrouck, Freddy and Decostere, Annemie},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keywords     = {TURBOT SCOPHTHALMUS-MAXIMUS,DICENTRARCHUS-LABRAX L.,LACTIC-ACID BACTERIA,COD GADUS-MORHUA,YOLK-SAC LARVAE,LISTONELLA-ANGUILLARUM,PROBIOTIC BACTERIA,SENEGALENSIS KAUP,CHALLENGE TEST,FOOD-CHAIN},
  language     = {eng},
  pages        = {9},
  title        = {Experimental infection model for vibriosis in Dover sole (Solea solea) larvae as an aid in studying its pathogenesis and alternative treatments},
  url          = {http://dx.doi.org/10.1186/s13567-018-0520-3},
  volume       = {49},
  year         = {2018},
}

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