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Diatom PtCPF1 is a new cryptochrome/photolyase family member with DNA repair and transcription regulation activity

(2009) EMBO REPORTS. 10(6). p.655-661
Author
Organization
Abstract
Members of the cryptochrome/photolyase family (CPF) are widely distributed throughout all kingdoms, and encode photosensitive proteins that typically show either photoreceptor or DNA repair activity. Animal and plant cryptochromes have lost DNA repair activity and now perform specialized photoperceptory functions, for example, plant cryptochromes regulate growth and circadian rhythms, whereas mammalian and insect cryptochromes act as transcriptional repressors that control the circadian clock. However, the functional differentiation between photolyases and cryptochromes is now being questioned. Here, we show that the PtCPF1 protein from the marine diatom Phaeodactylum tricornutum shows 6-4 photoproduct repair activity and can act as a transcriptional repressor of the circadian clock in a heterologous mammalian cell system. Conversely, it seems to have a wide role in blue-light-regulated gene expression in diatoms. The protein might therefore represent a missing link in the evolution of CPFs, and act as a novel ultraviolet/blue light sensor in marine environments.
Keywords
cryptochrome, 6-4 photolyase, photoreceptor evolution, diatom, DNA repair, BLUE-LIGHT PHOTORECEPTORS, PHOTOLYASE/CRYPTOCHROME FAMILY, THALASSIOSIRA-PSEUDONANA, GENE-EXPRESSION, CIRCADIAN CLOCK, HIGHER-PLANTS, IDENTIFICATION, PHOTOLYASE, GENOME, COMPONENTS

Citation

Please use this url to cite or link to this publication:

MLA
Coesel, Sacha, Manuela Mangogna, Tomoko Ishikawa, et al. “Diatom PtCPF1 Is a New Cryptochrome/photolyase Family Member with DNA Repair and Transcription Regulation Activity.” EMBO REPORTS 10.6 (2009): 655–661. Print.
APA
Coesel, S., Mangogna, M., Ishikawa, T., Heijde, M., Rogato, A., Finazzi, G., Todo, T., et al. (2009). Diatom PtCPF1 is a new cryptochrome/photolyase family member with DNA repair and transcription regulation activity. EMBO REPORTS, 10(6), 655–661.
Chicago author-date
Coesel, Sacha, Manuela Mangogna, Tomoko Ishikawa, Marc Heijde, Alessandra Rogato, Giovanni Finazzi, Takeshi Todo, Chris Bowler, and Angela Falciatore. 2009. “Diatom PtCPF1 Is a New Cryptochrome/photolyase Family Member with DNA Repair and Transcription Regulation Activity.” Embo Reports 10 (6): 655–661.
Chicago author-date (all authors)
Coesel, Sacha, Manuela Mangogna, Tomoko Ishikawa, Marc Heijde, Alessandra Rogato, Giovanni Finazzi, Takeshi Todo, Chris Bowler, and Angela Falciatore. 2009. “Diatom PtCPF1 Is a New Cryptochrome/photolyase Family Member with DNA Repair and Transcription Regulation Activity.” Embo Reports 10 (6): 655–661.
Vancouver
1.
Coesel S, Mangogna M, Ishikawa T, Heijde M, Rogato A, Finazzi G, et al. Diatom PtCPF1 is a new cryptochrome/photolyase family member with DNA repair and transcription regulation activity. EMBO REPORTS. 2009;10(6):655–61.
IEEE
[1]
S. Coesel et al., “Diatom PtCPF1 is a new cryptochrome/photolyase family member with DNA repair and transcription regulation activity,” EMBO REPORTS, vol. 10, no. 6, pp. 655–661, 2009.
@article{8551765,
  abstract     = {Members of the cryptochrome/photolyase family (CPF) are widely distributed throughout all kingdoms, and encode photosensitive proteins that typically show either photoreceptor or DNA repair activity. Animal and plant cryptochromes have lost DNA repair activity and now perform specialized photoperceptory functions, for example, plant cryptochromes regulate growth and circadian rhythms, whereas mammalian and insect cryptochromes act as transcriptional repressors that control the circadian clock. However, the functional differentiation between photolyases and cryptochromes is now being questioned. Here, we show that the PtCPF1 protein from the marine diatom Phaeodactylum tricornutum shows 6-4 photoproduct repair activity and can act as a transcriptional repressor of the circadian clock in a heterologous mammalian cell system. Conversely, it seems to have a wide role in blue-light-regulated gene expression in diatoms. The protein might therefore represent a missing link in the evolution of CPFs, and act as a novel ultraviolet/blue light sensor in marine environments.},
  author       = {Coesel, Sacha and Mangogna, Manuela and Ishikawa, Tomoko and Heijde, Marc and Rogato, Alessandra and Finazzi, Giovanni and Todo, Takeshi and Bowler, Chris and Falciatore, Angela},
  issn         = {1469-221X},
  journal      = {EMBO REPORTS},
  keywords     = {cryptochrome,6-4 photolyase,photoreceptor evolution,diatom,DNA repair,BLUE-LIGHT PHOTORECEPTORS,PHOTOLYASE/CRYPTOCHROME FAMILY,THALASSIOSIRA-PSEUDONANA,GENE-EXPRESSION,CIRCADIAN CLOCK,HIGHER-PLANTS,IDENTIFICATION,PHOTOLYASE,GENOME,COMPONENTS},
  language     = {eng},
  number       = {6},
  pages        = {655--661},
  title        = {Diatom PtCPF1 is a new cryptochrome/photolyase family member with DNA repair and transcription regulation activity},
  url          = {http://dx.doi.org/10.1038/embor.2009.59},
  volume       = {10},
  year         = {2009},
}

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