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The auxin-regulated CrRLK1L kinase ERULUS controls cell wall composition during root hair tip growth

(2018) CURRENT BIOLOGY. 28(5). p.722-732
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Abstract
Root hairs facilitate a plant's ability to acquire soil anchorage and nutrients. Root hair growth is regulated by the plant hormone auxin and dependent on localized synthesis, secretion, and modification of the root hair tip cell wall. However, the exact cell wall regulators in root hairs controlled by auxin have yet to be determined. In this study, we describe the characterization of ERULUS (ERU), an auxin-induced Arabidopsis receptor-like kinase, whose expression is directly regulated by ARF7 and ARF19 transcription factors. ERU belongs to the Catharanthus roseus RECEPTOR-LIKE KINASE 1-LIKE (CrRLK1L) subfamily of putative cell wall sensor proteins. Imaging of a fluorescent fusion protein revealed that ERU is localized to the apical root hair plasma membrane. ERU regulates cell wall composition in root hairs and modulates pectin dynamics through negative control of pectin methylesterase (PME) activity. Mutant eru (-/-) root hairs accumulate de-esterified homogalacturonan and exhibit aberrant pectin Ca2+-binding site oscillations and increased PME activity. Up to 80% of the eru root hair phenotype is rescued by pharmacological supplementation with a PME-inhibiting catechin extract. ERU transcription is altered in specific cell wall-related root hair mutants, suggesting that it is a target for feedback regulation. Loss of ERU alters the phosphorylation status of FERONIA and H+-ATPases 1/2, regulators of apoplastic pH. Furthermore, H+-ATPases 1/2 and ERU are differentially phosphorylated in response to auxin. We conclude that ERULUS is a key auxin-controlled regulator of cell wall composition and pectin dynamics during root hair tip growth.
Keywords
TRANSFORM INFRARED-SPECTROSCOPY, ARABIDOPSIS-THALIANA, PLASMA-MEMBRANE, POLAR GROWTH, POLLEN TUBES, PLANT, PECTIN, PHOSPHORYLATION, XYLOGLUCAN, INHIBITION

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Chicago
Schoenaers, Sébastjen, Daria Balcerowicz, Gordon Breen, Kristine Hill, Malgorzata Zdanio, Gregory Mouille, Tara J Holman, et al. 2018. “The Auxin-regulated CrRLK1L Kinase ERULUS Controls Cell Wall Composition During Root Hair Tip Growth.” Current Biology 28 (5): 722–732.
APA
Schoenaers, Sébastjen, Balcerowicz, D., Breen, G., Hill, K., Zdanio, M., Mouille, G., Holman, T. J., et al. (2018). The auxin-regulated CrRLK1L kinase ERULUS controls cell wall composition during root hair tip growth. CURRENT BIOLOGY, 28(5), 722–732.
Vancouver
1.
Schoenaers S, Balcerowicz D, Breen G, Hill K, Zdanio M, Mouille G, et al. The auxin-regulated CrRLK1L kinase ERULUS controls cell wall composition during root hair tip growth. CURRENT BIOLOGY. 2018;28(5):722–32.
MLA
Schoenaers, Sébastjen et al. “The Auxin-regulated CrRLK1L Kinase ERULUS Controls Cell Wall Composition During Root Hair Tip Growth.” CURRENT BIOLOGY 28.5 (2018): 722–732. Print.
@article{8551638,
  abstract     = {Root hairs facilitate a plant's ability to acquire soil anchorage and nutrients. Root hair growth is regulated by the plant hormone auxin and dependent on localized synthesis, secretion, and modification of the root hair tip cell wall. However, the exact cell wall regulators in root hairs controlled by auxin have yet to be determined. In this study, we describe the characterization of ERULUS (ERU), an auxin-induced Arabidopsis receptor-like kinase, whose expression is directly regulated by ARF7 and ARF19 transcription factors. ERU belongs to the Catharanthus roseus RECEPTOR-LIKE KINASE 1-LIKE (CrRLK1L) subfamily of putative cell wall sensor proteins. Imaging of a fluorescent fusion protein revealed that ERU is localized to the apical root hair plasma membrane. ERU regulates cell wall composition in root hairs and modulates pectin dynamics through negative control of pectin methylesterase (PME) activity. Mutant eru (-/-) root hairs accumulate de-esterified homogalacturonan and exhibit aberrant pectin Ca2+-binding site oscillations and increased PME activity. Up to 80% of the eru root hair phenotype is rescued by pharmacological supplementation with a PME-inhibiting catechin extract. ERU transcription is altered in specific cell wall-related root hair mutants, suggesting that it is a target for feedback regulation. Loss of ERU alters the phosphorylation status of FERONIA and H+-ATPases 1/2, regulators of apoplastic pH. Furthermore, H+-ATPases 1/2 and ERU are differentially phosphorylated in response to auxin. We conclude that ERULUS is a key auxin-controlled regulator of cell wall composition and pectin dynamics during root hair tip growth.},
  author       = {Schoenaers, Sébastjen and Balcerowicz, Daria and Breen, Gordon and Hill, Kristine and Zdanio, Malgorzata and Mouille, Gregory and Holman, Tara J and Oh, Jaesung and Wilson, Michael H and Nikonorova, Natalia and Vu, Lam Dai and De Smet, Ive and Swarup, Ranjan and De Vos, Winnok and Pintelon, Isabel and Adriaensen, Dirk and Grierson, Claire and Bennett, Malcolm J and Vissenberg, Kris},
  issn         = {0960-9822},
  journal      = {CURRENT BIOLOGY},
  keywords     = {TRANSFORM INFRARED-SPECTROSCOPY,ARABIDOPSIS-THALIANA,PLASMA-MEMBRANE,POLAR GROWTH,POLLEN TUBES,PLANT,PECTIN,PHOSPHORYLATION,XYLOGLUCAN,INHIBITION},
  language     = {eng},
  number       = {5},
  pages        = {722--732},
  title        = {The auxin-regulated CrRLK1L kinase ERULUS controls cell wall composition during root hair tip growth},
  url          = {http://dx.doi.org/10.1016/j.cub.2018.01.050},
  volume       = {28},
  year         = {2018},
}

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