# Random and aligned plasma-activated nanofibers of different diameters : impact on cell morphology and proliferation

Charlot Philips (UGent), Rouba Ghobeira (UGent), Heidi Declercq (UGent), Pieter Cools (UGent), Nathalie De Geyter (UGent), Rino Morent (UGent) and Maria Cornelissen (UGent)
Author
Organization
Abstract
INTRODUCTION: Poly-ε-caprolactone (PCL) is an interesting polymer for tissue engineering purposes due to its good mechanical properties and tunable degradation rate. However, its hydrophobicity raises a big challenge for cell adhesion and proliferation. To overcome this problem, plasma surface treatment could be used. Moreover, surface topography is also described to play an important role in cellular interaction. Therefore, in this study, PCL nanofibers of different diameters and orientations were produced and cellular interaction was evaluated for both untreated and plasma-activated nanofibers. MATERIALS AND METHODS: Random and aligned PCL nanofibers of three different diameters were produced by electrospinning and subsequently plasma-activated. Furthermore, cellular interaction was evaluated by seeding adipose-derived stem cells onto the nanofibers. Cell morphology was evaluated by means of scanning electron microscopy, while cell survival and proliferation was evaluated with live/dead staining and MTT assays. RESULTS AND DISCUSSION: Fiber orientation had a clear influence on cell morphology, with irregular-shaped cells on the random nanofibers and more elongated cells on the aligned nanofibers. Cell proliferation, on the other hand, was similar for random and aligned nanofibers. Due to the incorporation of functional groups, plasma treatment of the PCL nanofibers resulted in higher proliferation rates as compared to untreated samples, both for random and aligned nanofibers. In case of the untreated samples, higher nanofiber diameters seemed to have a positive influence on cell proliferation. For the plasma-treated samples, however, this could not be observed. CONCLUSIONS: To improve cellular interaction with PCL nanofibers, either plasma-treatment or an increase of surface roughness can be employed, but a combination of both has no synergistic effect.

## Citation

Chicago
Philips, Charlot, Rouba Ghobeira, Heidi Declercq, Pieter Cools, Nathalie De Geyter, Rino Morent, and Maria Cornelissen. 2017. “Random and Aligned Plasma-activated Nanofibers of Different Diameters : Impact on Cell Morphology and Proliferation.” In AMBA 2017 : Advanced Materials for Biomedical Applications.
APA
Philips, Charlot, Ghobeira, R., Declercq, H., Cools, P., De Geyter, N., Morent, R., & Cornelissen, M. (2017). Random and aligned plasma-activated nanofibers of different diameters : impact on cell morphology and proliferation. AMBA 2017 : advanced materials for biomedical applications. Presented at the Conference on Advanced Materials for Biomedical Applications (AMBA 2017).
Vancouver
1.
Philips C, Ghobeira R, Declercq H, Cools P, De Geyter N, Morent R, et al. Random and aligned plasma-activated nanofibers of different diameters : impact on cell morphology and proliferation. AMBA 2017 : advanced materials for biomedical applications. 2017.
MLA
Philips, Charlot, Rouba Ghobeira, Heidi Declercq, et al. “Random and Aligned Plasma-activated Nanofibers of Different Diameters : Impact on Cell Morphology and Proliferation.” AMBA 2017 : Advanced Materials for Biomedical Applications. 2017. Print.
@inproceedings{8551213,
abstract     = {INTRODUCTION: Poly-\ensuremath{\epsilon}-caprolactone (PCL) is an interesting polymer for tissue engineering purposes due to its good mechanical properties and tunable degradation rate. However, its hydrophobicity raises a big challenge for cell adhesion and proliferation. To overcome this problem, plasma surface treatment could be used. Moreover, surface topography is also described to play an important role in cellular interaction. Therefore, in this study, PCL nanofibers of different diameters and orientations were produced and cellular interaction was evaluated for both untreated and plasma-activated nanofibers.
MATERIALS AND METHODS: Random and aligned PCL nanofibers of three different diameters were produced by electrospinning and subsequently plasma-activated. Furthermore, cellular interaction was evaluated by seeding adipose-derived stem cells onto the nanofibers. Cell morphology was evaluated by means of scanning electron microscopy, while cell survival and proliferation was evaluated with live/dead staining and MTT assays.
RESULTS AND DISCUSSION: Fiber orientation had a clear influence on cell morphology, with irregular-shaped cells on the random nanofibers and more elongated cells on the aligned nanofibers. Cell proliferation, on the other hand, was similar for random and aligned nanofibers. Due to the incorporation of functional groups, plasma treatment of the PCL nanofibers resulted in higher proliferation rates as compared to untreated samples, both for random and aligned nanofibers. In case of the untreated samples, higher nanofiber diameters seemed to have a positive influence on cell proliferation. For the plasma-treated samples, however, this could not be observed.
CONCLUSIONS: To improve cellular interaction with PCL nanofibers, either plasma-treatment or an increase of surface roughness can be employed, but a combination of both has no synergistic effect.},
author       = {Philips, Charlot and Ghobeira, Rouba and Declercq, Heidi and Cools, Pieter and De Geyter, Nathalie and Morent, Rino and Cornelissen, Maria},
booktitle    = {AMBA 2017 : advanced materials for biomedical applications},
language     = {eng},
location     = {Ghent, Belgium},
title        = {Random and aligned plasma-activated nanofibers of different diameters : impact on cell morphology and proliferation},
year         = {2017},
}