Advanced search
1 file | 3.61 MB

Semi-automated digital measurement as the method of choice for beta cell mass analysis

(2018) PLOS ONE. 13(2).
Author
Organization
Abstract
Pancreas injury by partial duct ligation (PDL) activates beta cell differentiation and proliferation in adult mouse pancreas but remains controversial regarding the anticipated increase in beta cell volume. Several reports unable to show beta cell volume augmentation in PDL pancreas used automated digital image analysis software. We hypothesized that fully automatic beta cell morphometry without manual micrograph artifact remediation introduces bias and therefore might be responsible for reported discrepancies and controversy. However, our present results prove that standard digital image processing with automatic thresholding is sufficiently robust albeit less sensitive and less adequate to demonstrate a significant increase in beta cell volume in PDL versus Sham-operated pancreas. We therefore conclude that other confounding factors such as quality of surgery, selection of samples based on relative abundance of the transcription factor Neurogenin 3 (Ngn3) and tissue processing give rise to inter-laboratory inconsistencies in beta cell volume quantification in PDL pancreas.
Keywords
IMAGE-ANALYSIS, SOFTWARE

Downloads

  • journal.pone.0191249.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 3.61 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Coppens, Violette, Gunter Leuckx, Yves Heremans, Willem Staels, Yannick Verdonck, Luc Baeyens, Nico De Leu, and Harry Heimberg. 2018. “Semi-automated Digital Measurement as the Method of Choice for Beta Cell Mass Analysis.” Plos One 13 (2).
APA
Coppens, V., Leuckx, G., Heremans, Y., Staels, W., Verdonck, Y., Baeyens, L., De Leu, N., et al. (2018). Semi-automated digital measurement as the method of choice for beta cell mass analysis. PLOS ONE, 13(2).
Vancouver
1.
Coppens V, Leuckx G, Heremans Y, Staels W, Verdonck Y, Baeyens L, et al. Semi-automated digital measurement as the method of choice for beta cell mass analysis. PLOS ONE. 2018;13(2).
MLA
Coppens, Violette, Gunter Leuckx, Yves Heremans, et al. “Semi-automated Digital Measurement as the Method of Choice for Beta Cell Mass Analysis.” PLOS ONE 13.2 (2018): n. pag. Print.
@article{8550082,
  abstract     = {Pancreas injury by partial duct ligation (PDL) activates beta cell differentiation and proliferation in adult mouse pancreas but remains controversial regarding the anticipated increase in beta cell volume. Several reports unable to show beta cell volume augmentation in PDL pancreas used automated digital image analysis software. We hypothesized that fully automatic beta cell morphometry without manual micrograph artifact remediation introduces bias and therefore might be responsible for reported discrepancies and controversy. However, our present results prove that standard digital image processing with automatic thresholding is sufficiently robust albeit less sensitive and less adequate to demonstrate a significant increase in beta cell volume in PDL versus Sham-operated pancreas. We therefore conclude that other confounding factors such as quality of surgery, selection of samples based on relative abundance of the transcription factor Neurogenin 3 (Ngn3) and tissue processing give rise to inter-laboratory inconsistencies in beta cell volume quantification in PDL pancreas.},
  articleno    = {e0191249},
  author       = {Coppens, Violette and Leuckx, Gunter and Heremans, Yves and Staels, Willem and Verdonck, Yannick and Baeyens, Luc and De Leu, Nico and Heimberg, Harry},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {IMAGE-ANALYSIS,SOFTWARE},
  language     = {eng},
  number       = {2},
  pages        = {11},
  title        = {Semi-automated digital measurement as the method of choice for beta cell mass analysis},
  url          = {http://dx.doi.org/10.1371/journal.pone.0191249},
  volume       = {13},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: