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Environmental screening of multi-class antimicrobial drug residues in salt and fresh water ecosystems using targeted and untargeted high resolution mass spectrometry

Tess Goessens UGent, Steve Huysman UGent, Peter Goethals UGent, Ann Dumoulin UGent, Lynn Vanhaecke UGent and Siska Croubels UGent (2018) Proceedings of the 23rd National Symposium for Applied Biological Sciences.
abstract
In recent years, the prevalence of antimicrobial drug residues in the aquatic environment has gained special attention due to the potential development, spread and persistence of antimicrobial resistance. Moreover, antimicrobial drug residues can alter the growth of microorganisms that are important for the ecologically balanced food chain and the conservation of biodiversity. Despite the aforementioned concerns, European legislation does not yet provide environmental quality standards and technical specifications for evaluating the status of antimicrobial drug residues in the aquatic environment. Therefore, the aim of this study was to develop and validate a novel multi-residue method for the simultaneous determination of 64 targeted antimicrobial drugs in salt and fresh water. Therefore, proper internal standards were selected and a suitable extraction method based on solid-phase extraction (Waters Oasis HLB 200 mg, 6 ml) was designed, assisted by fractional factorial design. A broad polarity range of compounds was covered (log Kow from 5 to 12) including major representatives of the following classes: trimethoprim (n=1), sulfonamides (n=8), tetracyclines (n=7), aminoglycosides (n=4), quinolones (n=11), macrolides (n=4), lincosamides (n=1), nitrofurans (n=5), polymyxins (n=1), penicillins (n=9), cefalosporins (n=8), carbapenems (n=1), pleuromutilins (n=1) and phenicols (n=3). All analytes were successfully separated with an ultra-high performance liquid chromatograph (UHPLC), using a 1.9 µm Hypersil Gold column (100 mm x 2.1 mm) and detected in full-scan by a HR(-Q-Exactive™) mass spectrometer (HRMS). Moreover, chromatographic and mass spectrometric variables were carefully optimized. Thereafter, the developed UHPLC-HRMS method was validated according to CD 2002/657/EC for evaluating its performance (i.e. specificity, selectivity, linearity, trueness and precision). Results will be presented at the conference meeting. The major advantage of this newly developed method, lies in its ability of identifying additionally suspected and unknown compounds (not limited to the targeted list of compounds). Finally, this work will be an important step towards environmental monitoring programs and as such contribute to the European Water Framework Directive. Acknowledgements: This research is supported by the Special Research Fund of Ghent University grant number BOF16-GOA-024.08
Please use this url to cite or link to this publication:
author
organization
year
type
conference (poster)
publication status
published
subject
keyword
antimicrobial, salt water, fresh water, HRMS, environmental, targeted, untargeted
in
Proceedings of the 23rd National Symposium for Applied Biological Sciences
conference name
23rd National Symposium for Applied Biological Sciences
conference location
Brussels
conference start
2018-02-08
conference end
2018-02-08
project
Mitigating an amphibian pandemic by embedding biodiversity conservation in contaminant exposed agricultural landscapes
language
English
UGent publication?
yes
classification
U
id
8549933
handle
http://hdl.handle.net/1854/LU-8549933
date created
2018-02-13 13:34:01
date last changed
2018-02-13 13:41:57
@inproceedings{8549933,
  abstract     = {In recent years, the prevalence of antimicrobial drug residues in the aquatic environment has gained special attention due to the potential development, spread and persistence of antimicrobial resistance. Moreover, antimicrobial drug residues can alter the growth of microorganisms that are important for the ecologically balanced food chain and the conservation of biodiversity. Despite the aforementioned concerns, European legislation does not yet provide environmental quality standards and technical specifications for evaluating the status of antimicrobial drug residues in the aquatic environment.
Therefore, the aim of this study was to develop and validate a novel multi-residue method for the simultaneous determination of 64 targeted antimicrobial drugs in salt and fresh water. Therefore, proper internal standards were selected and a suitable extraction method based on solid-phase extraction (Waters Oasis HLB 200 mg, 6 ml) was designed, assisted by fractional factorial design. A broad polarity range of compounds was covered (log Kow from 5 to 12) including major representatives of the following classes: trimethoprim (n=1), sulfonamides (n=8), tetracyclines (n=7), aminoglycosides (n=4), quinolones (n=11), macrolides (n=4), lincosamides (n=1), nitrofurans (n=5), polymyxins (n=1), penicillins (n=9), cefalosporins (n=8), carbapenems (n=1), pleuromutilins (n=1) and phenicols (n=3). All analytes were successfully separated with an ultra-high performance liquid chromatograph (UHPLC), using a 1.9 {\textmu}m Hypersil Gold column (100 mm x 2.1 mm) and detected in full-scan by a HR(-Q-Exactive{\texttrademark}) mass spectrometer (HRMS). Moreover, chromatographic and mass spectrometric variables were carefully optimized. Thereafter, the developed UHPLC-HRMS method was validated according to CD 2002/657/EC for evaluating its performance (i.e. specificity, selectivity, linearity, trueness and precision). Results will be presented at the conference meeting.
The major advantage of this newly developed method, lies in its ability of identifying additionally suspected and unknown compounds (not limited to the targeted list of compounds). Finally, this work will be an important step towards environmental monitoring programs and as such contribute to the European Water Framework Directive. 
Acknowledgements: This research is supported by the Special Research Fund of Ghent University grant number BOF16-GOA-024.08
},
  author       = {Goessens, Tess and Huysman, Steve and Goethals, Peter and Dumoulin, Ann and Vanhaecke, Lynn and Croubels, Siska},
  booktitle    = {Proceedings of the 23rd National Symposium for Applied Biological Sciences},
  keyword      = {antimicrobial,salt water,fresh water,HRMS,environmental,targeted,untargeted},
  language     = {eng},
  location     = {Brussels},
  title        = {Environmental screening of multi-class antimicrobial drug residues in salt and fresh water ecosystems using targeted and untargeted high resolution mass spectrometry},
  year         = {2018},
}

Chicago
Goessens, Tess, Steve Huysman, Peter Goethals, Ann Dumoulin, Lynn Vanhaecke, and Siska Croubels. 2018. “Environmental Screening of Multi-class Antimicrobial Drug Residues in Salt and Fresh Water Ecosystems Using Targeted and Untargeted High Resolution Mass Spectrometry.” In Proceedings of the 23rd National Symposium for Applied Biological Sciences.
APA
Goessens, Tess, Huysman, S., Goethals, P., Dumoulin, A., Vanhaecke, L., & Croubels, S. (2018). Environmental screening of multi-class antimicrobial drug residues in salt and fresh water ecosystems using targeted and untargeted high resolution mass spectrometry. Proceedings of the 23rd National Symposium for Applied Biological Sciences. Presented at the 23rd National Symposium for Applied Biological Sciences.
Vancouver
1.
Goessens T, Huysman S, Goethals P, Dumoulin A, Vanhaecke L, Croubels S. Environmental screening of multi-class antimicrobial drug residues in salt and fresh water ecosystems using targeted and untargeted high resolution mass spectrometry. Proceedings of the 23rd National Symposium for Applied Biological Sciences. 2018.
MLA
Goessens, Tess, Steve Huysman, Peter Goethals, et al. “Environmental Screening of Multi-class Antimicrobial Drug Residues in Salt and Fresh Water Ecosystems Using Targeted and Untargeted High Resolution Mass Spectrometry.” Proceedings of the 23rd National Symposium for Applied Biological Sciences. 2018. Print.