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Immortalized porcine mesenchymal cells derived from nasal mucosa, lungs, lymph nodes, spleen and bone marrow retain their stemness properties and trigger the expression of siglec-1 in co-cultured blood monocytic cells

Abubakar Garba UGent, Lowiese Desmarets, Delphine Acar UGent, Bert Devriendt UGent and Hans Nauwynck UGent (2017) PLOS ONE. 12(10).
abstract
Mesenchymal stromal cells have been isolated from different sources. They are multipotent cells capable of differentiating into many different cell types, including osteocytes, chondrocytes and adipocytes. They possess a therapeutic potential in the management of immune disorders and the repair of damaged tissues. Previous work in our laboratory showed an increase of the percentages of CD172a(+), CD14(+), CD163(+), Siglec-1(+), CD4(+) and CD8(+) hematopoietic cells, when co-cultured with immortalized mesenchymal cells derived from bone marrow. The present work aimed to demonstrate the stemness properties of SV40-immortalized mesenchymal cells derived from nasal mucosa, lungs, spleen, lymph nodes and red bone marrow and their immunomodulatory effect on blood monocytes. Mesenchymal cells from nasal mucosa, lungs, spleen, lymph nodes and red bone marrow were isolated and successfully immortalized using simian virus 40 large T antigen (SV40LT) and later, co-cultured with blood monocytes, in order to examine their differentiation stage (expression of Siglec-1). Flow cytometric analysis revealed that the five mesenchymal cell lines were positive for mesenchymal cell markers CD105, CD44, CD90 and CD29, but lacked the expression of myeloid cell markers CD16 and CD11b. Growth analysis of the cells demonstrated that bone marrow derived-mesenchymal cells proliferated faster compared with those derived from the other tissues. All five mesenchymal cell lines co-cultured with blood monocytes for 1, 2 and 7 days triggered the expression of siglec-1 in the monocytes. In contrast, no siglec-1(+) cells were observed in monocyte cultures without mesenchymal cell lines. Mesenchymal cells isolated from nasal mucosa, lungs, spleen, lymph nodes and bone marrow were successfully immortalized and these cell lines retained their stemness properties and displayed immunomodulatory effects on blood monocytes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RESPIRATORY SYNDROME VIRUS, SUPPRESS T-LYMPHOCYTE, STROMAL CELLS, ALVEOLAR MACROPHAGES, IN-VIVO, MODEL, DIFFERENTIATION, TRANSPLANTATION, PROLIFERATION, ANTIGEN
journal title
PLOS ONE
PLoS One
volume
12
issue
10
article number
0186343
pages
18 pages
Web of Science type
Article
Web of Science id
000413054800021
ISSN
1932-6203
DOI
10.1371/journal.pone.0186343
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8549619
handle
http://hdl.handle.net/1854/LU-8549619
date created
2018-02-12 13:11:19
date last changed
2018-02-15 15:30:37
@article{8549619,
  abstract     = {Mesenchymal stromal cells have been isolated from different sources. They are multipotent cells capable of differentiating into many different cell types, including osteocytes, chondrocytes and adipocytes. They possess a therapeutic potential in the management of immune disorders and the repair of damaged tissues. Previous work in our laboratory showed an increase of the percentages of CD172a(+), CD14(+), CD163(+), Siglec-1(+), CD4(+) and CD8(+) hematopoietic cells, when co-cultured with immortalized mesenchymal cells derived from bone marrow. The present work aimed to demonstrate the stemness properties of SV40-immortalized mesenchymal cells derived from nasal mucosa, lungs, spleen, lymph nodes and red bone marrow and their immunomodulatory effect on blood monocytes. Mesenchymal cells from nasal mucosa, lungs, spleen, lymph nodes and red bone marrow were isolated and successfully immortalized using simian virus 40 large T antigen (SV40LT) and later, co-cultured with blood monocytes, in order to examine their differentiation stage (expression of Siglec-1). Flow cytometric analysis revealed that the five mesenchymal cell lines were positive for mesenchymal cell markers CD105, CD44, CD90 and CD29, but lacked the expression of myeloid cell markers CD16 and CD11b. Growth analysis of the cells demonstrated that bone marrow derived-mesenchymal cells proliferated faster compared with those derived from the other tissues. All five mesenchymal cell lines co-cultured with blood monocytes for 1, 2 and 7 days triggered the expression of siglec-1 in the monocytes. In contrast, no siglec-1(+) cells were observed in monocyte cultures without mesenchymal cell lines. Mesenchymal cells isolated from nasal mucosa, lungs, spleen, lymph nodes and bone marrow were successfully immortalized and these cell lines retained their stemness properties and displayed immunomodulatory effects on blood monocytes.},
  articleno    = {0186343},
  author       = {Garba, Abubakar and Desmarets, Lowiese and Acar, Delphine and Devriendt, Bert and Nauwynck, Hans},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {RESPIRATORY SYNDROME VIRUS,SUPPRESS T-LYMPHOCYTE,STROMAL CELLS,ALVEOLAR MACROPHAGES,IN-VIVO,MODEL,DIFFERENTIATION,TRANSPLANTATION,PROLIFERATION,ANTIGEN},
  language     = {eng},
  number       = {10},
  pages        = {18},
  title        = {Immortalized porcine mesenchymal cells derived from nasal mucosa, lungs, lymph nodes, spleen and bone marrow retain their stemness properties and trigger the expression of siglec-1 in co-cultured blood monocytic cells},
  url          = {http://dx.doi.org/10.1371/journal.pone.0186343},
  volume       = {12},
  year         = {2017},
}

Chicago
Garba, Abubakar, Lowiese Desmarets, Delphine Acar, Bert Devriendt, and Hans Nauwynck. 2017. “Immortalized Porcine Mesenchymal Cells Derived from Nasal Mucosa, Lungs, Lymph Nodes, Spleen and Bone Marrow Retain Their Stemness Properties and Trigger the Expression of Siglec-1 in Co-cultured Blood Monocytic Cells.” Plos One 12 (10).
APA
Garba, A., Desmarets, L., Acar, D., Devriendt, B., & Nauwynck, H. (2017). Immortalized porcine mesenchymal cells derived from nasal mucosa, lungs, lymph nodes, spleen and bone marrow retain their stemness properties and trigger the expression of siglec-1 in co-cultured blood monocytic cells. PLOS ONE, 12(10).
Vancouver
1.
Garba A, Desmarets L, Acar D, Devriendt B, Nauwynck H. Immortalized porcine mesenchymal cells derived from nasal mucosa, lungs, lymph nodes, spleen and bone marrow retain their stemness properties and trigger the expression of siglec-1 in co-cultured blood monocytic cells. PLOS ONE. 2017;12(10).
MLA
Garba, Abubakar, Lowiese Desmarets, Delphine Acar, et al. “Immortalized Porcine Mesenchymal Cells Derived from Nasal Mucosa, Lungs, Lymph Nodes, Spleen and Bone Marrow Retain Their Stemness Properties and Trigger the Expression of Siglec-1 in Co-cultured Blood Monocytic Cells.” PLOS ONE 12.10 (2017): n. pag. Print.