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Sickeningly sweet : L-rhamnose stimulates Flavobacterium columnare biofilm formation and virulence

(2017) JOURNAL OF FISH DISEASES. 40(11). p.1613-1624
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Abstract
Flavobacterium columnare, the causative agent of columnaris disease, causes substantial mortality worldwide in numerous freshwater finfish species. Due to its global significance and impact on the aquaculture industry continual efforts to better understand basic mechanisms that contribute to disease are urgently needed. The current work sought to evaluate the effect of L-rhamnose on the growth characteristics of F. columnare. While we initially did not observe any key changes during the total growth of F. columnare isolates tested when treated with L-rhamnose, it soon became apparent that the difference lies in the ability of this carbohydrate to facilitate the formation of biofilms. The addition of different concentrations of L-rhamnose consistently promoted the development of biofilms among different F. columnare isolates; however, it does not appear to be sufficient as a sole carbon source for biofilm growth. Our data also suggest that iron acquisition machinery is required for biofilm development. Finally, the addition of different concentrations of L-rhamnose to F. columnare prior to a laboratory challenge increased mortality rates in channel catfish (Ictalurus punctatus) as compared to controls. These results provide further evidence that biofilm formation is an integral virulence factor in the initiation of disease in fish.
Keywords
CATFISH ICTALURUS-PUNCTATUS, TRANSCRIPTIONAL REGULATORY NETWORK, IX, SECRETION SYSTEM, CHANNEL CATFISH, PSEUDOMONAS-AERUGINOSA, RAINBOW-TROUT, GLIDING MOTILITY, FISH PATHOGEN, GILL TISSUE, INFECTION, biofilm, Flavobacterium columnare, iron uptake, virulence

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Citation

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Chicago
Lange, MD, BD Farmer, Annelies Declercq, E Peatman, Annemie Decostere, and BH Beck. 2017. “Sickeningly Sweet : L-rhamnose Stimulates Flavobacterium Columnare Biofilm Formation and Virulence.” Journal of Fish Diseases 40 (11): 1613–1624.
APA
Lange, MD, Farmer, B., Declercq, A., Peatman, E., Decostere, A., & Beck, B. (2017). Sickeningly sweet : L-rhamnose stimulates Flavobacterium columnare biofilm formation and virulence. JOURNAL OF FISH DISEASES, 40(11), 1613–1624.
Vancouver
1.
Lange M, Farmer B, Declercq A, Peatman E, Decostere A, Beck B. Sickeningly sweet : L-rhamnose stimulates Flavobacterium columnare biofilm formation and virulence. JOURNAL OF FISH DISEASES. 2017;40(11):1613–24.
MLA
Lange, MD, BD Farmer, Annelies Declercq, et al. “Sickeningly Sweet : L-rhamnose Stimulates Flavobacterium Columnare Biofilm Formation and Virulence.” JOURNAL OF FISH DISEASES 40.11 (2017): 1613–1624. Print.
@article{8549394,
  abstract     = {Flavobacterium columnare, the causative agent of columnaris disease, causes substantial mortality worldwide in numerous freshwater finfish species. Due to its global significance and impact on the aquaculture industry continual efforts to better understand basic mechanisms that contribute to disease are urgently needed. The current work sought to evaluate the effect of L-rhamnose on the growth characteristics of F. columnare. While we initially did not observe any key changes during the total growth of F. columnare isolates tested when treated with L-rhamnose, it soon became apparent that the difference lies in the ability of this carbohydrate to facilitate the formation of biofilms. The addition of different concentrations of L-rhamnose consistently promoted the development of biofilms among different F. columnare isolates; however, it does not appear to be sufficient as a sole carbon source for biofilm growth. Our data also suggest that iron acquisition machinery is required for biofilm development. Finally, the addition of different concentrations of L-rhamnose to F. columnare prior to a laboratory challenge increased mortality rates in channel catfish (Ictalurus punctatus) as compared to controls. These results provide further evidence that biofilm formation is an integral virulence factor in the initiation of disease in fish.},
  author       = {Lange, MD and Farmer, BD and Declercq, Annelies and Peatman, E and Decostere, Annemie and Beck, BH},
  issn         = {0140-7775},
  journal      = {JOURNAL OF FISH DISEASES},
  language     = {eng},
  number       = {11},
  pages        = {1613--1624},
  title        = {Sickeningly sweet : L-rhamnose stimulates Flavobacterium columnare biofilm formation and virulence},
  url          = {http://dx.doi.org/10.1111/jfd.12629},
  volume       = {40},
  year         = {2017},
}

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