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High amorphous poly-beta-hydroxybutyrate (PHB) content in a probiotic Bacillus strain displays better protective effects in Vibrio-challenged gnotobiotic Artemia

(2018) AQUACULTURE. 487. p.15-21
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Abstract
In this study, the Bacillus sp. JL47, a superior PHB-accumulating Bacillus strain identified from the previous work, was tested for its protective effects in gnotobiotic Artemia franciscana during a pathogenic Vibrio campbellii challenge. The utilization of gnotobiotic Artemia is important in this experiment because any possible microbial interference (which are naturally present in conventional culture system) are eliminated in this model culture system, hence the interpretation of the results in this mechanistic study can be more conclusive. The survival of the Artemia fed the Bacillus sp. JL47 at 1 x 10(7) cells mL(-1) was significantly higher as compared with the challenged control and the survival was almost doubled when the dose was increased to 5 x 10(7) cells mL(-1). However, feeding the Artemia at 10(6) cells mL(-1) or lower showed no significant protective effects. Based on these densities, the estimated concentration of amorphous PHB showing a significant protection in Artemia was c. 2.44 mg L-1 and the effects were even better when the amorphous PHB level was increased to 12.19 mg L-1. Furthermore, feeding Bacillus sp. JL47 containing 55% amorphous PHB (on CDW) to Artemia showed a significantly higher survival in a Vibrio challenge relative to Bacillus containing 29% PHB. The data suggest that the amorphous PHB accumulated in the Bacillus sp. JL47 strain is an important determinant for the increased survival of challenged Artemia.
Keywords
Amorphous PHB, Probiotic Bacillus, Gnotobiotic Artemia, Vibrio campbellii, Challenge test, CHINESE MITTEN CRAB, EUROPEAN SEA BASS, GROWTH-PERFORMANCE, BACTERIAL CHALLENGE, ERIOCHEIR-SINENSIS, DISEASE RESISTANCE, LARVAE, FRANCISCANA, AQUACULTURE, CAMPBELLII

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Chicago
Laranja, Joseph, Peter De Schryver, Gladys Ludevese, Edgar C Amar, Maarten Aerts, Peter Vandamme, and Peter Bossier. 2018. “High Amorphous Poly-beta-hydroxybutyrate (PHB) Content in a Probiotic Bacillus Strain Displays Better Protective Effects in Vibrio-challenged Gnotobiotic Artemia.” Aquaculture 487: 15–21.
APA
Laranja, J., De Schryver, P., Ludevese, G., Amar, E. C., Aerts, M., Vandamme, P., & Bossier, P. (2018). High amorphous poly-beta-hydroxybutyrate (PHB) content in a probiotic Bacillus strain displays better protective effects in Vibrio-challenged gnotobiotic Artemia. AQUACULTURE, 487, 15–21.
Vancouver
1.
Laranja J, De Schryver P, Ludevese G, Amar EC, Aerts M, Vandamme P, et al. High amorphous poly-beta-hydroxybutyrate (PHB) content in a probiotic Bacillus strain displays better protective effects in Vibrio-challenged gnotobiotic Artemia. AQUACULTURE. 2018;487:15–21.
MLA
Laranja, Joseph, Peter De Schryver, Gladys Ludevese, et al. “High Amorphous Poly-beta-hydroxybutyrate (PHB) Content in a Probiotic Bacillus Strain Displays Better Protective Effects in Vibrio-challenged Gnotobiotic Artemia.” AQUACULTURE 487 (2018): 15–21. Print.
@article{8549162,
  abstract     = {In this study, the Bacillus sp. JL47, a superior PHB-accumulating Bacillus strain identified from the previous work, was tested for its protective effects in gnotobiotic Artemia franciscana during a pathogenic Vibrio campbellii challenge. The utilization of gnotobiotic Artemia is important in this experiment because any possible microbial interference (which are naturally present in conventional culture system) are eliminated in this model culture system, hence the interpretation of the results in this mechanistic study can be more conclusive. The survival of the Artemia fed the Bacillus sp. JL47 at 1 x 10(7) cells mL(-1) was significantly higher as compared with the challenged control and the survival was almost doubled when the dose was increased to 5 x 10(7) cells mL(-1). However, feeding the Artemia at 10(6) cells mL(-1) or lower showed no significant protective effects. Based on these densities, the estimated concentration of amorphous PHB showing a significant protection in Artemia was c. 2.44 mg L-1 and the effects were even better when the amorphous PHB level was increased to 12.19 mg L-1. Furthermore, feeding Bacillus sp. JL47 containing 55\% amorphous PHB (on CDW) to Artemia showed a significantly higher survival in a Vibrio challenge relative to Bacillus containing 29\% PHB. The data suggest that the amorphous PHB accumulated in the Bacillus sp. JL47 strain is an important determinant for the increased survival of challenged Artemia.},
  author       = {Laranja, Joseph and De Schryver, Peter and Ludevese, Gladys and Amar, Edgar C and Aerts, Maarten and Vandamme, Peter and Bossier, Peter},
  issn         = {0044-8486},
  journal      = {AQUACULTURE},
  keyword      = {Amorphous PHB,Probiotic Bacillus,Gnotobiotic Artemia,Vibrio campbellii,Challenge test,CHINESE MITTEN CRAB,EUROPEAN SEA BASS,GROWTH-PERFORMANCE,BACTERIAL CHALLENGE,ERIOCHEIR-SINENSIS,DISEASE RESISTANCE,LARVAE,FRANCISCANA,AQUACULTURE,CAMPBELLII},
  language     = {eng},
  pages        = {15--21},
  title        = {High amorphous poly-beta-hydroxybutyrate (PHB) content in a probiotic Bacillus strain displays better protective effects in Vibrio-challenged gnotobiotic Artemia},
  url          = {http://dx.doi.org/10.1016/j.aquaculture.2018.01.005},
  volume       = {487},
  year         = {2018},
}

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