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Residues Arg568 and Phe592 contribute to an antigenic surface for anti-ADAMTS13 antibodies in the spacer domain

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Abstract
Background : The majority of patients diagnosed with thrombotic thrombocytopenic purpura have autoantibodies directed towards the spacer domain of ADAMTS13. Design and Methods : In this study we explored the epitope specificity and immunoglobulin class and immunoglobulin G subclass distribution of anti-ADAMTS13 antibodies. The epitope specificity of anti-spacer domain antibodies was examined using plasma from 48 patients with acute acquired thrombotic thrombocytopenic purpura by means of immunoprecipitation of ADAMTS13 variants containing single or multiple alanine substitutions. Using similar methods, we also determined the presence of anti-TSP2-8 and CUB1-2 domain antibodies in this cohort of patients. Results : Antibody profiling revealed that anti-ADAMTS13 immunoglobulin G1 and immunoglobulin G4 predominate in plasma of patients with acquired thrombotic thrombocytopenic purpura. Analysis of anti-spacer domain antibodies revealed that Arg568 and Phe592, in addition to residues Arg660, Tyr661, and Tyr665, also contribute to an antigenic surface in the spacer domain. The majority of patients (90%) lost reactivity towards the spacer domain following introduction of multiple alanine substitutions at Arg568, Phe592, Arg660, Tyr661 and Tyr665. Anti-TSP2-8 and anti-CUB1-2 domain-directed antibodies were present in, respectively, 17% and 35% of the patients' samples analyzed. Conclusions : Immunoglobulin G directed towards a single antigenic surface comprising residues Arg568, Phe592, Arg660, Tyr661 and Tyr665 predominates in the plasma of patients with acquired thrombotic thrombocytopenic purpura.
Keywords
ADAMTS13, spacer domain, thrombotic thrombocytopenic purpura, antibodies, epitope, THROMBOTIC THROMBOCYTOPENIC PURPURA, VON-WILLEBRAND-FACTOR, ADAMTS13 ACTIVITY, BINDING-SITE, A2 DOMAIN, SUBSTRATE, CLEAVAGE, VWF, AUTOANTIBODIES, RECOGNITION

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Chicago
Pos, Wouter, Nicoletta Sorvillo, Rob Fijnheer, Hendrik Feys, Paul HP Kaijen, Gestur Vidarsson, and Jan Voorberg. 2011. “Residues Arg568 and Phe592 Contribute to an Antigenic Surface for anti-ADAMTS13 Antibodies in the Spacer Domain.” Haematologica-the Hematology Journal 96 (11): 1670–1677.
APA
Pos, Wouter, Sorvillo, N., Fijnheer, R., Feys, H., Kaijen, P. H., Vidarsson, G., & Voorberg, J. (2011). Residues Arg568 and Phe592 contribute to an antigenic surface for anti-ADAMTS13 antibodies in the spacer domain. HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, 96(11), 1670–1677.
Vancouver
1.
Pos W, Sorvillo N, Fijnheer R, Feys H, Kaijen PH, Vidarsson G, et al. Residues Arg568 and Phe592 contribute to an antigenic surface for anti-ADAMTS13 antibodies in the spacer domain. HAEMATOLOGICA-THE HEMATOLOGY JOURNAL. 2011;96(11):1670–7.
MLA
Pos, Wouter, Nicoletta Sorvillo, Rob Fijnheer, et al. “Residues Arg568 and Phe592 Contribute to an Antigenic Surface for anti-ADAMTS13 Antibodies in the Spacer Domain.” HAEMATOLOGICA-THE HEMATOLOGY JOURNAL 96.11 (2011): 1670–1677. Print.
@article{8549100,
  abstract     = {Background : The majority of patients diagnosed with thrombotic thrombocytopenic purpura have autoantibodies directed towards the spacer domain of ADAMTS13. 
Design and Methods : In this study we explored the epitope specificity and immunoglobulin class and immunoglobulin G subclass distribution of anti-ADAMTS13 antibodies. The epitope specificity of anti-spacer domain antibodies was examined using plasma from 48 patients with acute acquired thrombotic thrombocytopenic purpura by means of immunoprecipitation of ADAMTS13 variants containing single or multiple alanine substitutions. Using similar methods, we also determined the presence of anti-TSP2-8 and CUB1-2 domain antibodies in this cohort of patients. 
Results : Antibody profiling revealed that anti-ADAMTS13 immunoglobulin G1 and immunoglobulin G4 predominate in plasma of patients with acquired thrombotic thrombocytopenic purpura. Analysis of anti-spacer domain antibodies revealed that Arg568 and Phe592, in addition to residues Arg660, Tyr661, and Tyr665, also contribute to an antigenic surface in the spacer domain. The majority of patients (90\%) lost reactivity towards the spacer domain following introduction of multiple alanine substitutions at Arg568, Phe592, Arg660, Tyr661 and Tyr665. Anti-TSP2-8 and anti-CUB1-2 domain-directed antibodies were present in, respectively, 17\% and 35\% of the patients' samples analyzed. 
Conclusions : Immunoglobulin G directed towards a single antigenic surface comprising residues Arg568, Phe592, Arg660, Tyr661 and Tyr665 predominates in the plasma of patients with acquired thrombotic thrombocytopenic purpura.},
  author       = {Pos, Wouter and Sorvillo, Nicoletta and Fijnheer, Rob and Feys, Hendrik and Kaijen, Paul HP and Vidarsson, Gestur and Voorberg, Jan},
  issn         = {0390-6078},
  journal      = {HAEMATOLOGICA-THE HEMATOLOGY JOURNAL},
  language     = {eng},
  number       = {11},
  pages        = {1670--1677},
  title        = {Residues Arg568 and Phe592 contribute to an antigenic surface for anti-ADAMTS13 antibodies in the spacer domain},
  url          = {http://dx.doi.org/10.3324/haematol.2010.036327},
  volume       = {96},
  year         = {2011},
}

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