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A dual-color quantum dots encoded frit-based immunoassay for visual detection of aflatoxin M-1 and pirlimycin residues in milk

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Abstract
Mycotoxins and antibacterial agents are the main chemical hazards that lead to several health problems. Nowadays, multiplex immunoassay is a primary goal throughout the world. Here, aflatoxin M-1, and pirlimycin were selected as models, and a novel dual colorimetric encoded frit-based immunoassay was developed for simultaneously screening of aflatoxin M-1, and pirlimycin residues in milk. This multiplex fit-based immunoassay combined two monoclonal antibodies to extend the spectrum of analytes and to enable detection of two classes of analytes in a single test. The cutoff values were 0.02 mu g/kg for aflatoxin M-1, and 0.5 mu g/kg for pirlimycin, which satisfied the requirement to measure the maximum residue levels. The novel colorimetric frit-based immunoassay has the advantage of high throughput, short analysis time, reduced overall cost per assay, and can be used as a rapid screening technique for simultaneously detecting aflatoxin M-1 and pirlimycin residues in milk.
Keywords
LINKED-IMMUNOSORBENT-ASSAY, EDIBLE ANIMAL-TISSUES, MONOCLONAL-ANTIBODY, UHPLC-MS/MS, FLUORESCENCE, CLINDAMYCIN, SULFONAMIDE, ZEARALENONE, MYCOTOXINS, PROTEIN, aflatoxin M-1, pirlimycin, immunoassay, milk, food safety

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Citation

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Chicago
Jiang, Wenxiao, Natalia Beloglazova, Pengjie Luo, Ping Guo, Guimiao Lin, and Xiaomei Wang. 2017. “A Dual-color Quantum Dots Encoded Frit-based Immunoassay for Visual Detection of Aflatoxin M-1 and Pirlimycin Residues in Milk.” Journal of Agricultural and Food Chemistry 65 (8): 1822–1828.
APA
Jiang, Wenxiao, Beloglazova, N., Luo, P., Guo, P., Lin, G., & Wang, X. (2017). A dual-color quantum dots encoded frit-based immunoassay for visual detection of aflatoxin M-1 and pirlimycin residues in milk. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 65(8), 1822–1828.
Vancouver
1.
Jiang W, Beloglazova N, Luo P, Guo P, Lin G, Wang X. A dual-color quantum dots encoded frit-based immunoassay for visual detection of aflatoxin M-1 and pirlimycin residues in milk. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2017;65(8):1822–8.
MLA
Jiang, Wenxiao, Natalia Beloglazova, Pengjie Luo, et al. “A Dual-color Quantum Dots Encoded Frit-based Immunoassay for Visual Detection of Aflatoxin M-1 and Pirlimycin Residues in Milk.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.8 (2017): 1822–1828. Print.
@article{8548518,
  abstract     = {Mycotoxins and antibacterial agents are the main chemical hazards that lead to several health problems. Nowadays, multiplex immunoassay is a primary goal throughout the world. Here, aflatoxin M-1, and pirlimycin were selected as models, and a novel dual colorimetric encoded frit-based immunoassay was developed for simultaneously screening of aflatoxin M-1, and pirlimycin residues in milk. This multiplex fit-based immunoassay combined two monoclonal antibodies to extend the spectrum of analytes and to enable detection of two classes of analytes in a single test. The cutoff values were 0.02 mu g/kg for aflatoxin M-1, and 0.5 mu g/kg for pirlimycin, which satisfied the requirement to measure the maximum residue levels. The novel colorimetric frit-based immunoassay has the advantage of high throughput, short analysis time, reduced overall cost per assay, and can be used as a rapid screening technique for simultaneously detecting aflatoxin M-1 and pirlimycin residues in milk.},
  author       = {Jiang, Wenxiao and Beloglazova, Natalia and Luo, Pengjie and Guo, Ping and Lin, Guimiao and Wang, Xiaomei},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keyword      = {LINKED-IMMUNOSORBENT-ASSAY,EDIBLE ANIMAL-TISSUES,MONOCLONAL-ANTIBODY,UHPLC-MS/MS,FLUORESCENCE,CLINDAMYCIN,SULFONAMIDE,ZEARALENONE,MYCOTOXINS,PROTEIN,aflatoxin M-1,pirlimycin,immunoassay,milk,food safety},
  language     = {eng},
  number       = {8},
  pages        = {1822--1828},
  title        = {A dual-color quantum dots encoded frit-based immunoassay for visual detection of aflatoxin M-1 and pirlimycin residues in milk},
  url          = {http://dx.doi.org/10.1021/acs.jafc.6b05337},
  volume       = {65},
  year         = {2017},
}

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