
A luminescence immunoassay test method for determining benzo[a]pyrene in natural water
- Author
- Natalia Beloglazova (UGent) , PS Shmelin, AS Novikova and IYu Goryacheva
- Organization
- Abstract
- A test method is developed for determining benzo[a]pyrene in natural water, based on the use of a polyethylene filter (frit) with adsorbed specific antibodies, placed within a transparent column. In passing a test solution, the analyte is adsorbed on the frit similarly to the process implemented in immunoaffinity preconcentration. The added conjugate of a labeled analyte takes the remained vacant binding sites of antibodies. Luminescent semiconductor nanoparticles (quantum dots) CdSe/ZnS, used as labels, enable visual determination under irradiation with UV light. The limit of detection for benzo[a]pyrene in water is similar to 0.5 ng/mL.
- Keywords
- POLYCYCLIC AROMATIC-HYDROCARBONS, SAMPLES, EMISSIONS, PYRENE, test methods, nanoanalytics, natural, technological, and potable water, luminescence, quantum dots
Downloads
-
(...).pdf
- full text
- |
- UGent only
- |
- |
- 262.60 KB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8548515
- MLA
- Beloglazova, Natalia, et al. “A Luminescence Immunoassay Test Method for Determining Benzo[a]Pyrene in Natural Water.” JOURNAL OF ANALYTICAL CHEMISTRY, vol. 72, no. 6, 2017, pp. 597–601, doi:10.1134/S106193481706003X.
- APA
- Beloglazova, N., Shmelin, P., Novikova, A., & Goryacheva, Iy. (2017). A luminescence immunoassay test method for determining benzo[a]pyrene in natural water. JOURNAL OF ANALYTICAL CHEMISTRY, 72(6), 597–601. https://doi.org/10.1134/S106193481706003X
- Chicago author-date
- Beloglazova, Natalia, PS Shmelin, AS Novikova, and IYu Goryacheva. 2017. “A Luminescence Immunoassay Test Method for Determining Benzo[a]Pyrene in Natural Water.” JOURNAL OF ANALYTICAL CHEMISTRY 72 (6): 597–601. https://doi.org/10.1134/S106193481706003X.
- Chicago author-date (all authors)
- Beloglazova, Natalia, PS Shmelin, AS Novikova, and IYu Goryacheva. 2017. “A Luminescence Immunoassay Test Method for Determining Benzo[a]Pyrene in Natural Water.” JOURNAL OF ANALYTICAL CHEMISTRY 72 (6): 597–601. doi:10.1134/S106193481706003X.
- Vancouver
- 1.Beloglazova N, Shmelin P, Novikova A, Goryacheva Iy. A luminescence immunoassay test method for determining benzo[a]pyrene in natural water. JOURNAL OF ANALYTICAL CHEMISTRY. 2017;72(6):597–601.
- IEEE
- [1]N. Beloglazova, P. Shmelin, A. Novikova, and Iy. Goryacheva, “A luminescence immunoassay test method for determining benzo[a]pyrene in natural water,” JOURNAL OF ANALYTICAL CHEMISTRY, vol. 72, no. 6, pp. 597–601, 2017.
@article{8548515, abstract = {{A test method is developed for determining benzo[a]pyrene in natural water, based on the use of a polyethylene filter (frit) with adsorbed specific antibodies, placed within a transparent column. In passing a test solution, the analyte is adsorbed on the frit similarly to the process implemented in immunoaffinity preconcentration. The added conjugate of a labeled analyte takes the remained vacant binding sites of antibodies. Luminescent semiconductor nanoparticles (quantum dots) CdSe/ZnS, used as labels, enable visual determination under irradiation with UV light. The limit of detection for benzo[a]pyrene in water is similar to 0.5 ng/mL.}}, author = {{Beloglazova, Natalia and Shmelin, PS and Novikova, AS and Goryacheva, IYu}}, issn = {{1061-9348}}, journal = {{JOURNAL OF ANALYTICAL CHEMISTRY}}, keywords = {{POLYCYCLIC AROMATIC-HYDROCARBONS,SAMPLES,EMISSIONS,PYRENE,test methods,nanoanalytics,natural,technological,and potable water,luminescence,quantum dots}}, language = {{eng}}, number = {{6}}, pages = {{597--601}}, title = {{A luminescence immunoassay test method for determining benzo[a]pyrene in natural water}}, url = {{http://dx.doi.org/10.1134/S106193481706003X}}, volume = {{72}}, year = {{2017}}, }
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: