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A luminescence immunoassay test method for determining benzo[a]pyrene in natural water

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Abstract
A test method is developed for determining benzo[a]pyrene in natural water, based on the use of a polyethylene filter (frit) with adsorbed specific antibodies, placed within a transparent column. In passing a test solution, the analyte is adsorbed on the frit similarly to the process implemented in immunoaffinity preconcentration. The added conjugate of a labeled analyte takes the remained vacant binding sites of antibodies. Luminescent semiconductor nanoparticles (quantum dots) CdSe/ZnS, used as labels, enable visual determination under irradiation with UV light. The limit of detection for benzo[a]pyrene in water is similar to 0.5 ng/mL.
Keywords
POLYCYCLIC AROMATIC-HYDROCARBONS, SAMPLES, EMISSIONS, PYRENE, test methods, nanoanalytics, natural, technological, and potable water, luminescence, quantum dots

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MLA
Beloglazova, Natalia, PS Shmelin, AS Novikova, et al. “A Luminescence Immunoassay Test Method for Determining Benzo[a]pyrene in Natural Water.” JOURNAL OF ANALYTICAL CHEMISTRY 72.6 (2017): 597–601. Print.
APA
Beloglazova, Natalia, Shmelin, P., Novikova, A., & Goryacheva, Iy. (2017). A luminescence immunoassay test method for determining benzo[a]pyrene in natural water. JOURNAL OF ANALYTICAL CHEMISTRY, 72(6), 597–601.
Chicago author-date
Beloglazova, Natalia, PS Shmelin, AS Novikova, and Iyu Goryacheva. 2017. “A Luminescence Immunoassay Test Method for Determining Benzo[a]pyrene in Natural Water.” Journal of Analytical Chemistry 72 (6): 597–601.
Chicago author-date (all authors)
Beloglazova, Natalia, PS Shmelin, AS Novikova, and Iyu Goryacheva. 2017. “A Luminescence Immunoassay Test Method for Determining Benzo[a]pyrene in Natural Water.” Journal of Analytical Chemistry 72 (6): 597–601.
Vancouver
1.
Beloglazova N, Shmelin P, Novikova A, Goryacheva Iy. A luminescence immunoassay test method for determining benzo[a]pyrene in natural water. JOURNAL OF ANALYTICAL CHEMISTRY. 2017;72(6):597–601.
IEEE
[1]
N. Beloglazova, P. Shmelin, A. Novikova, and Iy. Goryacheva, “A luminescence immunoassay test method for determining benzo[a]pyrene in natural water,” JOURNAL OF ANALYTICAL CHEMISTRY, vol. 72, no. 6, pp. 597–601, 2017.
@article{8548515,
  abstract     = {{A test method is developed for determining benzo[a]pyrene in natural water, based on the use of a polyethylene filter (frit) with adsorbed specific antibodies, placed within a transparent column. In passing a test solution, the analyte is adsorbed on the frit similarly to the process implemented in immunoaffinity preconcentration. The added conjugate of a labeled analyte takes the remained vacant binding sites of antibodies. Luminescent semiconductor nanoparticles (quantum dots) CdSe/ZnS, used as labels, enable visual determination under irradiation with UV light. The limit of detection for benzo[a]pyrene in water is similar to 0.5 ng/mL.}},
  author       = {{Beloglazova, Natalia and Shmelin, PS and Novikova, AS and Goryacheva, IYu}},
  issn         = {{1061-9348}},
  journal      = {{JOURNAL OF ANALYTICAL CHEMISTRY}},
  keywords     = {{POLYCYCLIC AROMATIC-HYDROCARBONS,SAMPLES,EMISSIONS,PYRENE,test methods,nanoanalytics,natural,technological,and potable water,luminescence,quantum dots}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{597--601}},
  title        = {{A luminescence immunoassay test method for determining benzo[a]pyrene in natural water}},
  url          = {{http://dx.doi.org/10.1134/S106193481706003X}},
  volume       = {{72}},
  year         = {{2017}},
}

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