A luminescence immunoassay test method for determining benzo[a]pyrene in natural water
- Author
- Natalia Beloglazova (UGent) , PS Shmelin, AS Novikova and IYu Goryacheva
- Organization
- Abstract
- A test method is developed for determining benzo[a]pyrene in natural water, based on the use of a polyethylene filter (frit) with adsorbed specific antibodies, placed within a transparent column. In passing a test solution, the analyte is adsorbed on the frit similarly to the process implemented in immunoaffinity preconcentration. The added conjugate of a labeled analyte takes the remained vacant binding sites of antibodies. Luminescent semiconductor nanoparticles (quantum dots) CdSe/ZnS, used as labels, enable visual determination under irradiation with UV light. The limit of detection for benzo[a]pyrene in water is similar to 0.5 ng/mL.
- Keywords
- POLYCYCLIC AROMATIC-HYDROCARBONS, SAMPLES, EMISSIONS, PYRENE, test methods, nanoanalytics, natural, technological, and potable water, luminescence, quantum dots
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8548515
- MLA
- Beloglazova, Natalia, PS Shmelin, AS Novikova, et al. “A Luminescence Immunoassay Test Method for Determining Benzo[a]pyrene in Natural Water.” JOURNAL OF ANALYTICAL CHEMISTRY 72.6 (2017): 597–601. Print.
- APA
- Beloglazova, Natalia, Shmelin, P., Novikova, A., & Goryacheva, Iy. (2017). A luminescence immunoassay test method for determining benzo[a]pyrene in natural water. JOURNAL OF ANALYTICAL CHEMISTRY, 72(6), 597–601.
- Chicago author-date
- Beloglazova, Natalia, PS Shmelin, AS Novikova, and Iyu Goryacheva. 2017. “A Luminescence Immunoassay Test Method for Determining Benzo[a]pyrene in Natural Water.” Journal of Analytical Chemistry 72 (6): 597–601.
- Chicago author-date (all authors)
- Beloglazova, Natalia, PS Shmelin, AS Novikova, and Iyu Goryacheva. 2017. “A Luminescence Immunoassay Test Method for Determining Benzo[a]pyrene in Natural Water.” Journal of Analytical Chemistry 72 (6): 597–601.
- Vancouver
- 1.Beloglazova N, Shmelin P, Novikova A, Goryacheva Iy. A luminescence immunoassay test method for determining benzo[a]pyrene in natural water. JOURNAL OF ANALYTICAL CHEMISTRY. 2017;72(6):597–601.
- IEEE
- [1]N. Beloglazova, P. Shmelin, A. Novikova, and Iy. Goryacheva, “A luminescence immunoassay test method for determining benzo[a]pyrene in natural water,” JOURNAL OF ANALYTICAL CHEMISTRY, vol. 72, no. 6, pp. 597–601, 2017.
@article{8548515,
abstract = {{A test method is developed for determining benzo[a]pyrene in natural water, based on the use of a polyethylene filter (frit) with adsorbed specific antibodies, placed within a transparent column. In passing a test solution, the analyte is adsorbed on the frit similarly to the process implemented in immunoaffinity preconcentration. The added conjugate of a labeled analyte takes the remained vacant binding sites of antibodies. Luminescent semiconductor nanoparticles (quantum dots) CdSe/ZnS, used as labels, enable visual determination under irradiation with UV light. The limit of detection for benzo[a]pyrene in water is similar to 0.5 ng/mL.}},
author = {{Beloglazova, Natalia and Shmelin, PS and Novikova, AS and Goryacheva, IYu}},
issn = {{1061-9348}},
journal = {{JOURNAL OF ANALYTICAL CHEMISTRY}},
keywords = {{POLYCYCLIC AROMATIC-HYDROCARBONS,SAMPLES,EMISSIONS,PYRENE,test methods,nanoanalytics,natural,technological,and potable water,luminescence,quantum dots}},
language = {{eng}},
number = {{6}},
pages = {{597--601}},
title = {{A luminescence immunoassay test method for determining benzo[a]pyrene in natural water}},
url = {{http://dx.doi.org/10.1134/S106193481706003X}},
volume = {{72}},
year = {{2017}},
}
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