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Volumetric absorptive microsampling at home as an alternative tool for the monitoring of HbA1c in diabetes patients

Nick Verougstraete, Bruno Lapauw UGent, Sara Van Aken, Joris Delanghe UGent, Christophe Stove UGent and Veronique Stove UGent (2017) CLINICAL CHEMISTRY AND LABORATORY MEDICINE. 55(3). p.462-469
abstract
Background: Microsampling techniques have several advantages over traditional blood collection. Dried blood spot (DBS) sampling and blood collection with heparinized capillaries are the standard techniques. Volumetric absorptive microsampling (VAMS) is a novel technique that collects a fixed volume of blood by applying an absorbent tip to a blood drop. In the present study we explored the feasibility of HbA(1c) monitoring with VAMS sampling at home and analysis in the laboratory. Methods: Diabetic patients were enrolled in this study during consultation with the endocrinologist. A venous (adults) or capillary (children) sample was taken for immediate HbA(1c) analysis. DBS (n=1)and dried VAMS (n=2) were collected at home and sent to the laboratory. For 25 pediatric patients one VAMS was collected during consultation for immediate analysis (without drying), referred to as "wet VAMS". HbA(1c) analyses were performed on a Tosoh HLC-723 G8 high-performance liquid chromatography (HPLC) analyzer. Results: The median time between sampling at home and analysis was 3 days. Results of HbA(1c) in dried VAMS showed a poor agreement with venous/capillary blood collected in hospital (concordance correlation coefficient CCC=0.72). Similar observations were found with standard DBS. An excellent agreement was obtained between HbA(1c) results on wet VAMS (CCC=0.996) and standard blood samples. Patients experienced VAMS and DBS as easy and convenient to use. Conclusions: Utilizing equipment standard available in the clinical laboratory, the use of home-sampled dried VAMS and DBS is not a reliable tool for the monitoring of HbA(1c). However, perfect agreement between HbA(1c) measured on wet VAMS and capillary microsamples was obtained.
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author
organization
alternative title
Volumetric absorptive microsampling at home as an alternative tool for the monitoring of HbA(1c) in diabetes patients
year
type
journalArticle (original)
publication status
published
subject
keyword
DRIED BLOOD SPOT, DENSITY-LIPOPROTEIN CHOLESTEROL, QUANTITATIVE, BIOANALYSIS, HEMOGLOBIN A(1C), COLLECTION, HEMATOCRIT, SAMPLES, ISSUES, A1C, diabetes, dried blood spots, hemoglobin A(1c), home-sampling, volumetric, absorptive microsampling
journal title
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
Clin. Chem. Lab. Med.
volume
55
issue
3
pages
462 - 469
Web of Science type
Article
Web of Science id
000394145100029
ISSN
1434-6621
1437-4331
DOI
10.1515/cclm-2016-0411
language
English
UGent publication?
yes
classification
A1
additional info
the last two authors contributed equally to this work
copyright statement
I have transferred the copyright for this publication to the publisher
id
8548478
handle
http://hdl.handle.net/1854/LU-8548478
date created
2018-02-08 13:26:05
date last changed
2018-06-21 09:00:13
@article{8548478,
  abstract     = {Background: Microsampling techniques have several advantages over traditional blood collection. Dried blood spot (DBS) sampling and blood collection with heparinized capillaries are the standard techniques. Volumetric absorptive microsampling (VAMS) is a novel technique that collects a fixed volume of blood by applying an absorbent tip to a blood drop. In the present study we explored the feasibility of HbA(1c) monitoring with VAMS sampling at home and analysis in the laboratory. 
Methods: Diabetic patients were enrolled in this study during consultation with the endocrinologist. A venous (adults) or capillary (children) sample was taken for immediate HbA(1c) analysis. DBS (n=1)and dried VAMS (n=2) were collected at home and sent to the laboratory. For 25 pediatric patients one VAMS was collected during consultation for immediate analysis (without drying), referred to as {\textacutedbl}wet VAMS{\textacutedbl}. HbA(1c) analyses were performed on a Tosoh HLC-723 G8 high-performance liquid chromatography (HPLC) analyzer. 
Results: The median time between sampling at home and analysis was 3 days. Results of HbA(1c) in dried VAMS showed a poor agreement with venous/capillary blood collected in hospital (concordance correlation coefficient CCC=0.72). Similar observations were found with standard DBS. An excellent agreement was obtained between HbA(1c) results on wet VAMS (CCC=0.996) and standard blood samples. Patients experienced VAMS and DBS as easy and convenient to use. 
Conclusions: Utilizing equipment standard available in the clinical laboratory, the use of home-sampled dried VAMS and DBS is not a reliable tool for the monitoring of HbA(1c). However, perfect agreement between HbA(1c) measured on wet VAMS and capillary microsamples was obtained.},
  author       = {Verougstraete, Nick and Lapauw, Bruno and Van Aken, Sara and Delanghe, Joris and Stove, Christophe and Stove, Veronique},
  issn         = {1434-6621},
  journal      = {CLINICAL CHEMISTRY AND LABORATORY MEDICINE},
  keyword      = {DRIED BLOOD SPOT,DENSITY-LIPOPROTEIN CHOLESTEROL,QUANTITATIVE,BIOANALYSIS,HEMOGLOBIN A(1C),COLLECTION,HEMATOCRIT,SAMPLES,ISSUES,A1C,diabetes,dried blood spots,hemoglobin A(1c),home-sampling,volumetric,absorptive microsampling},
  language     = {eng},
  number       = {3},
  pages        = {462--469},
  title        = {Volumetric absorptive microsampling at home as an alternative tool for the monitoring of HbA1c in diabetes patients},
  url          = {http://dx.doi.org/10.1515/cclm-2016-0411},
  volume       = {55},
  year         = {2017},
}

Chicago
Verougstraete, Nick, Bruno Lapauw, Sara Van Aken, Joris Delanghe, Christophe Stove, and Veronique Stove. 2017. “Volumetric Absorptive Microsampling at Home as an Alternative Tool for the Monitoring of HbA1c in Diabetes Patients.” Clinical Chemistry and Laboratory Medicine 55 (3): 462–469.
APA
Verougstraete, N., Lapauw, B., Van Aken, S., Delanghe, J., Stove, C., & Stove, V. (2017). Volumetric absorptive microsampling at home as an alternative tool for the monitoring of HbA1c in diabetes patients. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 55(3), 462–469.
Vancouver
1.
Verougstraete N, Lapauw B, Van Aken S, Delanghe J, Stove C, Stove V. Volumetric absorptive microsampling at home as an alternative tool for the monitoring of HbA1c in diabetes patients. CLINICAL CHEMISTRY AND LABORATORY MEDICINE. 2017;55(3):462–9.
MLA
Verougstraete, Nick, Bruno Lapauw, Sara Van Aken, et al. “Volumetric Absorptive Microsampling at Home as an Alternative Tool for the Monitoring of HbA1c in Diabetes Patients.” CLINICAL CHEMISTRY AND LABORATORY MEDICINE 55.3 (2017): 462–469. Print.