Advanced search
1 file | 1.63 MB Add to list

Screening of quorum sensing peptides for biological effects in neuronal cells

Yorick Janssens (UGent) , Evelien Wynendaele (UGent) , Frederick Verbeke (UGent) , Nathan Debunne (UGent) , Bert Gevaert (UGent) , Kurt Audenaert (UGent) , Christophe Van De Wiele (UGent) and Bart De Spiegeleer (UGent)
(2018) PEPTIDES. 101. p.150-156
Author
Organization
Abstract
Quorum sensing peptides (QSP) are an important class of bacterial peptides which can have an effect on human host cells. These peptides are used by bacteria to communicate with each other. Some QSP are able to cross the blood-brain barrier and reach the brain parenchyma. However, nothing is known about the effects of these peptides in the brain. Therefore, 85 quorum sensing peptides were screened on six different neuronal cell lines using MTT toxicity, neurite differentiation, cytokine production and morphology as biological outcomes. This primary screening resulted in 22 peptides with effects observed on neuronal cell lines, indicating a possible role in the gut-brain axis. Four peptides (Q138, Q143, Q180 and Q212) showed induction of neurite outgrowth while two peptides (Q162 and Q208) inhibited NGF-induced neurite outgrowth in PC12 cells. Eight peptides (Q25, Q135, Q137, Q146, Q151, Q165, Q208 and Q298) induced neurite outgrowth in human SH-SY5Y neuroblastoma cells. Two peptides (Q13 and Q52) were toxic for SH-SY5Y cells and one (Q123) for BV-2 microglia cells based on the MTT assay. Six peptides had an effect on BV-2 microglia, Q180, Q184 and Q191 were able to induce IL-6 expression and Q164, Q192 and Q208 induced NO production. Finally, Q75 and Q147 treated C8D1A astrocytes demonstrated a higher fraction of round cells. Overall, these in vitro screening study results indicate for the first time possible effects of QSP on neuronal cells.
Keywords
Quorum sensing peptides, Screening, Biological effects, Neuronal cells, Microbiome

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.63 MB

Citation

Please use this url to cite or link to this publication:

MLA
Janssens, Yorick, Evelien Wynendaele, Frederick Verbeke, et al. “Screening of Quorum Sensing Peptides for Biological Effects in Neuronal Cells.” PEPTIDES 101 (2018): 150–156. Print.
APA
Janssens, Yorick, Wynendaele, E., Verbeke, F., Debunne, N., Gevaert, B., Audenaert, K., Van De Wiele, C., et al. (2018). Screening of quorum sensing peptides for biological effects in neuronal cells. PEPTIDES, 101, 150–156.
Chicago author-date
Janssens, Yorick, Evelien Wynendaele, Frederick Verbeke, Nathan Debunne, Bert Gevaert, Kurt Audenaert, Christophe Van De Wiele, and Bart De Spiegeleer. 2018. “Screening of Quorum Sensing Peptides for Biological Effects in Neuronal Cells.” Peptides 101: 150–156.
Chicago author-date (all authors)
Janssens, Yorick, Evelien Wynendaele, Frederick Verbeke, Nathan Debunne, Bert Gevaert, Kurt Audenaert, Christophe Van De Wiele, and Bart De Spiegeleer. 2018. “Screening of Quorum Sensing Peptides for Biological Effects in Neuronal Cells.” Peptides 101: 150–156.
Vancouver
1.
Janssens Y, Wynendaele E, Verbeke F, Debunne N, Gevaert B, Audenaert K, et al. Screening of quorum sensing peptides for biological effects in neuronal cells. PEPTIDES. 2018;101:150–6.
IEEE
[1]
Y. Janssens et al., “Screening of quorum sensing peptides for biological effects in neuronal cells,” PEPTIDES, vol. 101, pp. 150–156, 2018.
@article{8548297,
  abstract     = {Quorum sensing peptides (QSP) are an important class of bacterial peptides which can have an effect on human host cells. These peptides are used by bacteria to communicate with each other. Some QSP are able to cross the blood-brain barrier and reach the brain parenchyma. However, nothing is known about the effects of these peptides in the brain. Therefore, 85 quorum sensing peptides were screened on six different neuronal cell lines using MTT toxicity, neurite differentiation, cytokine production and morphology as biological outcomes. This primary screening resulted in 22 peptides with effects observed on neuronal cell lines, indicating a possible role in the gut-brain axis. Four peptides (Q138, Q143, Q180 and Q212) showed induction of neurite outgrowth while two peptides (Q162 and Q208) inhibited NGF-induced neurite outgrowth in PC12 cells. Eight peptides (Q25, Q135, Q137, Q146, Q151, Q165, Q208 and Q298) induced neurite outgrowth in human SH-SY5Y neuroblastoma cells. Two peptides (Q13 and Q52) were toxic for SH-SY5Y cells and one (Q123) for BV-2 microglia cells based on the MTT assay. Six peptides had an effect on BV-2 microglia, Q180, Q184 and Q191 were able to induce IL-6 expression and Q164, Q192 and Q208 induced NO production. Finally, Q75 and Q147 treated C8D1A astrocytes demonstrated a higher fraction of round cells. Overall, these in vitro screening study results indicate for the first time possible effects of QSP on neuronal cells.},
  author       = {Janssens, Yorick and Wynendaele, Evelien and Verbeke, Frederick and Debunne, Nathan and Gevaert, Bert and Audenaert, Kurt and Van De Wiele, Christophe and De Spiegeleer, Bart},
  issn         = {0196-9781},
  journal      = {PEPTIDES},
  keywords     = {Quorum sensing peptides,Screening,Biological effects,Neuronal cells,Microbiome},
  language     = {eng},
  pages        = {150--156},
  title        = {Screening of quorum sensing peptides for biological effects in neuronal cells},
  url          = {http://dx.doi.org/10.1016/j.peptides.2018.01.013},
  volume       = {101},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: