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Propagation-based phase-contrast synchrotron imaging of aortic dissection in mice : from individual elastic lamella to 3D analysis

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Abstract
In order to show the advantage and potential of propagation-based phase-contrast synchrotron imaging in vascular pathology research, we analyzed aortic medial ruptures in BAPN/AngII-infused mice, a mouse model for aortic dissection. Ascending and thoraco-abdominal samples from n = 3 control animals and n = 10 BAPN/AngII-infused mice (after 3, 7 and 14 days of infusion, total of 24 samples) were scanned. A steep increase in the number of ruptures was already noted after 3 days of BAPN/AngII-infusion. The largest ruptures were found at the latest time points. 133 ruptures affected only the first lamella while 135 ruptures affected multiple layers. Medial ruptures through all lamellar layers, leading to false channel formation and intramural hematoma, occurred only in the thoraco-abdominal aorta and interlamellar hematoma formation in the ascending aorta could be directly related to ruptures of the innermost lamellae. The advantages of this technique are (i) ultra-high resolution that allows to visualize the individual elastic lamellae in the aorta; (ii) quantitative and qualitative analysis of medial ruptures; (iii) 3D analysis of the complete aorta; (iv) high contrast for qualitative information extraction, reducing the need for histology coupes; (v) earlier detection of (micro-) ruptures.
Keywords
Animal disease models, Aortic diseases, Imaging

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Chicago
Logghe, Gerlinde, Bram Trachet, Lydia Aslanidou, Pablo Villaneuva-Perez, Julie De Backer, Nikolaos Stergiopulos, Marco Stampanoni, Hiroki Aoki, and Patrick Segers. 2018. “Propagation-based Phase-contrast Synchrotron Imaging of Aortic Dissection in Mice : from Individual Elastic Lamella to 3D Analysis.” Scientific Reports 8 (1).
APA
Logghe, G., Trachet, B., Aslanidou, L., Villaneuva-Perez, P., De Backer, J., Stergiopulos, N., Stampanoni, M., et al. (2018). Propagation-based phase-contrast synchrotron imaging of aortic dissection in mice : from individual elastic lamella to 3D analysis. SCIENTIFIC REPORTS, 8(1).
Vancouver
1.
Logghe G, Trachet B, Aslanidou L, Villaneuva-Perez P, De Backer J, Stergiopulos N, et al. Propagation-based phase-contrast synchrotron imaging of aortic dissection in mice : from individual elastic lamella to 3D analysis. SCIENTIFIC REPORTS. Springer Nature; 2018;8(1).
MLA
Logghe, Gerlinde, Bram Trachet, Lydia Aslanidou, et al. “Propagation-based Phase-contrast Synchrotron Imaging of Aortic Dissection in Mice : from Individual Elastic Lamella to 3D Analysis.” SCIENTIFIC REPORTS 8.1 (2018): n. pag. Print.
@article{8547801,
  abstract     = {In order to show the advantage and potential of propagation-based phase-contrast synchrotron imaging in vascular pathology research, we analyzed aortic medial ruptures in BAPN/AngII-infused mice, a mouse model for aortic dissection. Ascending and thoraco-abdominal samples from n\,=\,3 control animals and n\,=\,10 BAPN/AngII-infused mice (after 3, 7 and 14 days of infusion, total of 24 samples) were scanned. A steep increase in the number of ruptures was already noted after 3 days of BAPN/AngII-infusion. The largest ruptures were found at the latest time points. 133 ruptures affected only the first lamella while 135 ruptures affected multiple layers. Medial ruptures through all lamellar layers, leading to false channel formation and intramural hematoma, occurred only in the thoraco-abdominal aorta and interlamellar hematoma formation in the ascending aorta could be directly related to ruptures of the innermost lamellae. The advantages of this technique are (i) ultra-high resolution that allows to visualize the individual elastic lamellae in the aorta; (ii) quantitative and qualitative analysis of medial ruptures; (iii) 3D analysis of the complete aorta; (iv) high contrast for qualitative information extraction, reducing the need for histology coupes; (v) earlier detection of (micro-) ruptures.},
  author       = {Logghe, Gerlinde and Trachet, Bram and Aslanidou, Lydia and Villaneuva-Perez, Pablo and De Backer, Julie and Stergiopulos, Nikolaos and Stampanoni, Marco and Aoki, Hiroki and Segers, Patrick},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keyword      = {Animal disease models,Aortic diseases,Imaging},
  language     = {eng},
  number       = {1},
  pages        = {11},
  publisher    = {Springer Nature},
  title        = {Propagation-based phase-contrast synchrotron imaging of aortic dissection in mice : from individual elastic lamella to 3D analysis},
  url          = {http://dx.doi.org/10.1038/s41598-018-20673-x},
  volume       = {8},
  year         = {2018},
}

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