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Gold nanodome SERS platform for label-free detection of protease activity

Pieter Wuytens (UGent) , Hans Demol (UGent) , Nina Turk (UGent) , Kris Gevaert (UGent) , Andre Skirtach (UGent) , Mohamed Lamkanfi (UGent) and Roel Baets (UGent)
(2017) FARADAY DISCUSSIONS . 205. p.345-361
Author
Organization
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Center for nano- and biophotonics (NB-Photonics)
Abstract
Surface-enhanced Raman scattering provides a promising technology for sensitive and selective detection of protease activity by monitoring peptide cleavage. Not only are peptides and plasmonic hotspots similarly sized, Raman fingerprints also hold large potential for spectral multiplexing. Here, we use a gold-nanodome platform for real-time detection of trypsin activity on a CALNNYGGGGVRGNF substrate peptide. First, we investigate the spectral changes upon cleavage through the SERS signal of liquid-chromatography separated products. Next, we show that similar patterns are detected upon digesting surface-bound peptides. We demonstrate that the relative intensity of the fingerprints from aromatic amino acids before and after the cleavage site provides a robust figure of merit for the turnover rate. The presented method offers a generic approach for measuring protease activity, which is illustrated by developing an analogous substrate for endoproteinase Glu-C.
Keywords
ENHANCED RAMAN-SPECTROSCOPY, NANOPARTICLES, SCATTERING, TRYPSIN, PROBES, MICROSCOPY, MONOLAYERS, MOLECULES, PEPTIDES, SENSORS

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Citation

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Chicago
Wuytens, Pieter, Hans Demol, Nina Turk, Kris Gevaert, Andre Skirtach, Mohamed Lamkanfi, and Roel Baets. 2017. “Gold Nanodome SERS Platform for Label-free Detection of Protease Activity.” Faraday Discussions 205: 345–361.
APA
Wuytens, P., Demol, H., Turk, N., Gevaert, K., Skirtach, A., Lamkanfi, M., & Baets, R. (2017). Gold nanodome SERS platform for label-free detection of protease activity. FARADAY DISCUSSIONS , 205, 345–361.
Vancouver
1.
Wuytens P, Demol H, Turk N, Gevaert K, Skirtach A, Lamkanfi M, et al. Gold nanodome SERS platform for label-free detection of protease activity. FARADAY DISCUSSIONS . 2017;205:345–61.
MLA
Wuytens, Pieter, Hans Demol, Nina Turk, et al. “Gold Nanodome SERS Platform for Label-free Detection of Protease Activity.” FARADAY DISCUSSIONS 205 (2017): 345–361. Print.
@article{8547773,
  abstract     = {Surface-enhanced Raman scattering provides a promising technology for sensitive and selective detection of protease activity by monitoring peptide cleavage. Not only are peptides and plasmonic hotspots similarly sized, Raman fingerprints also hold large potential for spectral multiplexing. Here, we use a gold-nanodome platform for real-time detection of trypsin activity on a CALNNYGGGGVRGNF substrate peptide. First, we investigate the spectral changes upon cleavage through the SERS signal of liquid-chromatography separated products. Next, we show that similar patterns are detected upon digesting surface-bound peptides. We demonstrate that the relative intensity of the fingerprints from aromatic amino acids before and after the cleavage site provides a robust figure of merit for the turnover rate. The presented method offers a generic approach for measuring protease activity, which is illustrated by developing an analogous substrate for endoproteinase Glu-C.},
  author       = {Wuytens, Pieter and Demol, Hans and Turk, Nina and Gevaert, Kris and Skirtach, Andre and Lamkanfi, Mohamed and Baets, Roel},
  issn         = {1359-6640},
  journal      = {FARADAY DISCUSSIONS },
  language     = {eng},
  pages        = {345--361},
  title        = {Gold nanodome SERS platform for label-free detection of protease activity},
  url          = {http://dx.doi.org/10.1039/c7fd00124j},
  volume       = {205},
  year         = {2017},
}

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