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Immobilization of 2-deoxy-D-ribose-5-phosphate aldolase in polymeric thin films via the Langmuir-Schaefer technique

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Abstract
A synthetic protocol for the fabrication of ultrathin polymeric films containing the enzyme 2-deoxy-D-ribose-5-phosphate aldolase from Escherichia coli (DERA(EC)) is presented. Ultrathin enzymatically active films are useful for applications in which only small quantities of active material are needed and at the same time quick response and contact times without diffusion limitation are wanted. We show how DERA as an exemplary enzyme can be immobilized in a thin polymer layer at the air-water interface and transferred to a suitable support by the Langmuir-Schaefer technique under full conservation of enzymatic activity. The polymer in use is a poly(N-isopropylacrylamide-co-N-2-thiolactone acrylamide) (P(NIPAAm-co-TlaAm)) statistical copolymer in which the thiolactone units serve a multitude of purposes including hydrophobization of the polymer, covalent binding of the enzyme and the support and finally cross-linking of the polymer matrix. The application of this type of polymer keeps the whole approach simple as additional cocomponents such as cross-linkers are avoided.
Keywords
AIR-WATER-INTERFACE, ENZYME IMMOBILIZATION, ALDEHYDE TOLERANCE, MEMBRANE, REACTORS, BLODGETT-FILMS, BIOSENSORS, DEOXYRIBOALDOLASE, THIOLACTONE, IMPROVEMENT, STABILITY, Langmuir-Schaefer, enzyme immobilization, 2-deoxy-D-ribose-5-phosphate, aldolase, polymeric thin film, poly(N-isopropylacrylamide), thiolactone

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Chicago
Reinicke, Stefan, Huw C Rees, Pieter Espeel, Nane Vanparijs, Carolin Bisterfeld, Markus Dick, Ruben R Rosencrantz, et al. 2017. “Immobilization of 2-deoxy-D-ribose-5-phosphate Aldolase in Polymeric Thin Films via the Langmuir-Schaefer Technique.” Acs Applied Materials & Interfaces 9 (9): 8317–8326.
APA
Reinicke, S., Rees, H. C., Espeel, P., Vanparijs, N., Bisterfeld, C., Dick, M., Rosencrantz, R. R., et al. (2017). Immobilization of 2-deoxy-D-ribose-5-phosphate aldolase in polymeric thin films via the Langmuir-Schaefer technique. ACS APPLIED MATERIALS & INTERFACES, 9(9), 8317–8326.
Vancouver
1.
Reinicke S, Rees HC, Espeel P, Vanparijs N, Bisterfeld C, Dick M, et al. Immobilization of 2-deoxy-D-ribose-5-phosphate aldolase in polymeric thin films via the Langmuir-Schaefer technique. ACS APPLIED MATERIALS & INTERFACES. 2017;9(9):8317–26.
MLA
Reinicke, Stefan, Huw C Rees, Pieter Espeel, et al. “Immobilization of 2-deoxy-D-ribose-5-phosphate Aldolase in Polymeric Thin Films via the Langmuir-Schaefer Technique.” ACS APPLIED MATERIALS & INTERFACES 9.9 (2017): 8317–8326. Print.
@article{8547597,
  abstract     = {A synthetic protocol for the fabrication of ultrathin polymeric films containing the enzyme 2-deoxy-D-ribose-5-phosphate aldolase from Escherichia coli (DERA(EC)) is presented. Ultrathin enzymatically active films are useful for applications in which only small quantities of active material are needed and at the same time quick response and contact times without diffusion limitation are wanted. We show how DERA as an exemplary enzyme can be immobilized in a thin polymer layer at the air-water interface and transferred to a suitable support by the Langmuir-Schaefer technique under full conservation of enzymatic activity. The polymer in use is a poly(N-isopropylacrylamide-co-N-2-thiolactone acrylamide) (P(NIPAAm-co-TlaAm)) statistical copolymer in which the thiolactone units serve a multitude of purposes including hydrophobization of the polymer, covalent binding of the enzyme and the support and finally cross-linking of the polymer matrix. The application of this type of polymer keeps the whole approach simple as additional cocomponents such as cross-linkers are avoided.},
  author       = {Reinicke, Stefan and Rees, Huw C and Espeel, Pieter and Vanparijs, Nane and Bisterfeld, Carolin and Dick, Markus and Rosencrantz, Ruben R and Brezesinski, Gerald and De Geest, Bruno and Du Prez, Filip and Pietruszka, Joerg and Boeker, Alexander},
  issn         = {1944-8244},
  journal      = {ACS APPLIED MATERIALS \& INTERFACES},
  keyword      = {AIR-WATER-INTERFACE,ENZYME IMMOBILIZATION,ALDEHYDE TOLERANCE,MEMBRANE,REACTORS,BLODGETT-FILMS,BIOSENSORS,DEOXYRIBOALDOLASE,THIOLACTONE,IMPROVEMENT,STABILITY,Langmuir-Schaefer,enzyme immobilization,2-deoxy-D-ribose-5-phosphate,aldolase,polymeric thin film,poly(N-isopropylacrylamide),thiolactone},
  language     = {eng},
  number       = {9},
  pages        = {8317--8326},
  title        = {Immobilization of 2-deoxy-D-ribose-5-phosphate aldolase in polymeric thin films via the Langmuir-Schaefer technique},
  url          = {http://dx.doi.org/10.1021/acsami.6b13632},
  volume       = {9},
  year         = {2017},
}

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