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Immobilization of 2-deoxy-D-ribose-5-phosphate aldolase in polymeric thin films via the Langmuir-Schaefer technique

Stefan Reinicke, Huw C Rees, Pieter Espeel, Nane Vanparijs, Carolin Bisterfeld, Markus Dick, Ruben R Rosencrantz, Gerald Brezesinski, Bruno De Geest UGent, Filip Du Prez UGent, et al. (2017) ACS APPLIED MATERIALS & INTERFACES. 9(9). p.8317-8326
abstract
A synthetic protocol for the fabrication of ultrathin polymeric films containing the enzyme 2-deoxy-D-ribose-5-phosphate aldolase from Escherichia coli (DERA(EC)) is presented. Ultrathin enzymatically active films are useful for applications in which only small quantities of active material are needed and at the same time quick response and contact times without diffusion limitation are wanted. We show how DERA as an exemplary enzyme can be immobilized in a thin polymer layer at the air-water interface and transferred to a suitable support by the Langmuir-Schaefer technique under full conservation of enzymatic activity. The polymer in use is a poly(N-isopropylacrylamide-co-N-2-thiolactone acrylamide) (P(NIPAAm-co-TlaAm)) statistical copolymer in which the thiolactone units serve a multitude of purposes including hydrophobization of the polymer, covalent binding of the enzyme and the support and finally cross-linking of the polymer matrix. The application of this type of polymer keeps the whole approach simple as additional cocomponents such as cross-linkers are avoided.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
AIR-WATER-INTERFACE, ENZYME IMMOBILIZATION, ALDEHYDE TOLERANCE, MEMBRANE, REACTORS, BLODGETT-FILMS, BIOSENSORS, DEOXYRIBOALDOLASE, THIOLACTONE, IMPROVEMENT, STABILITY, Langmuir-Schaefer, enzyme immobilization, 2-deoxy-D-ribose-5-phosphate, aldolase, polymeric thin film, poly(N-isopropylacrylamide), thiolactone
journal title
ACS APPLIED MATERIALS & INTERFACES
ACS Appl. Mater. Interfaces
volume
9
issue
9
pages
8317 - 8326
Web of Science type
Article
Web of Science id
000396186000051
ISSN
1944-8244
DOI
10.1021/acsami.6b13632
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8547597
handle
http://hdl.handle.net/1854/LU-8547597
date created
2018-02-02 12:32:18
date last changed
2018-06-12 12:19:29
@article{8547597,
  abstract     = {A synthetic protocol for the fabrication of ultrathin polymeric films containing the enzyme 2-deoxy-D-ribose-5-phosphate aldolase from Escherichia coli (DERA(EC)) is presented. Ultrathin enzymatically active films are useful for applications in which only small quantities of active material are needed and at the same time quick response and contact times without diffusion limitation are wanted. We show how DERA as an exemplary enzyme can be immobilized in a thin polymer layer at the air-water interface and transferred to a suitable support by the Langmuir-Schaefer technique under full conservation of enzymatic activity. The polymer in use is a poly(N-isopropylacrylamide-co-N-2-thiolactone acrylamide) (P(NIPAAm-co-TlaAm)) statistical copolymer in which the thiolactone units serve a multitude of purposes including hydrophobization of the polymer, covalent binding of the enzyme and the support and finally cross-linking of the polymer matrix. The application of this type of polymer keeps the whole approach simple as additional cocomponents such as cross-linkers are avoided.},
  author       = {Reinicke, Stefan and Rees, Huw C and Espeel, Pieter and Vanparijs, Nane and Bisterfeld, Carolin and Dick, Markus and Rosencrantz, Ruben R and Brezesinski, Gerald and De Geest, Bruno and Du Prez, Filip and Pietruszka, Joerg and Boeker, Alexander},
  issn         = {1944-8244},
  journal      = {ACS APPLIED MATERIALS \& INTERFACES},
  keyword      = {AIR-WATER-INTERFACE,ENZYME IMMOBILIZATION,ALDEHYDE TOLERANCE,MEMBRANE,REACTORS,BLODGETT-FILMS,BIOSENSORS,DEOXYRIBOALDOLASE,THIOLACTONE,IMPROVEMENT,STABILITY,Langmuir-Schaefer,enzyme immobilization,2-deoxy-D-ribose-5-phosphate,aldolase,polymeric thin film,poly(N-isopropylacrylamide),thiolactone},
  language     = {eng},
  number       = {9},
  pages        = {8317--8326},
  title        = {Immobilization of 2-deoxy-D-ribose-5-phosphate aldolase in polymeric thin films via the Langmuir-Schaefer technique},
  url          = {http://dx.doi.org/10.1021/acsami.6b13632},
  volume       = {9},
  year         = {2017},
}

Chicago
Reinicke, Stefan, Huw C Rees, Pieter Espeel, Nane Vanparijs, Carolin Bisterfeld, Markus Dick, Ruben R Rosencrantz, et al. 2017. “Immobilization of 2-deoxy-D-ribose-5-phosphate Aldolase in Polymeric Thin Films via the Langmuir-Schaefer Technique.” Acs Applied Materials & Interfaces 9 (9): 8317–8326.
APA
Reinicke, S., Rees, H. C., Espeel, P., Vanparijs, N., Bisterfeld, C., Dick, M., Rosencrantz, R. R., et al. (2017). Immobilization of 2-deoxy-D-ribose-5-phosphate aldolase in polymeric thin films via the Langmuir-Schaefer technique. ACS APPLIED MATERIALS & INTERFACES, 9(9), 8317–8326.
Vancouver
1.
Reinicke S, Rees HC, Espeel P, Vanparijs N, Bisterfeld C, Dick M, et al. Immobilization of 2-deoxy-D-ribose-5-phosphate aldolase in polymeric thin films via the Langmuir-Schaefer technique. ACS APPLIED MATERIALS & INTERFACES. 2017;9(9):8317–26.
MLA
Reinicke, Stefan, Huw C Rees, Pieter Espeel, et al. “Immobilization of 2-deoxy-D-ribose-5-phosphate Aldolase in Polymeric Thin Films via the Langmuir-Schaefer Technique.” ACS APPLIED MATERIALS & INTERFACES 9.9 (2017): 8317–8326. Print.