Advanced search
1 file | 453.40 KB Add to list

Nasopharyngeal S. pneumoniae carriage and density in Belgian infants after 9 years of pneumococcal conjugate vaccine programme

(2018) VACCINE. 36(1). p.15-22
Author
Organization
Abstract
Background: In Belgium, the infant pneumococcal conjugate vaccine (PCV) programme changed from PCV7 (2007-2011) to PCV13 (2011-2015) and to PCV10 (2015-2016). A 3-year nasopharyngeal carriage study was initiated during the programme switch in 2016. Main objective of the year 1 assessment was to obtain a baseline measurement of pneumococcal carriage prevalence, carriage density, serotype distribution and antibiotic resistance. Materials/methods: Two infant populations aged 6-30 months and without use of antibiotics in the seven days prior to sampling were approached: (1) attending one of 85 randomly selected day-care centres (DCC); (2) presenting with AOM at study-trained general practitioners and paediatricians. Demographic and clinical characteristics were documented and a single nasopharyngeal swab was taken. S. pneumoniae were cultured, screened for antibiotic resistance and serotyped, and quantitative Taqman real-time PCR (qRT-PCR) targeting LytA was performed. Results: Culture-based (DCC: 462/760; 60.8%- AOM: 27/39; 69.2%) and LytA-based (DCC: 603/753; 80.1% - AOM: 32/39; 82.1%) carriage prevalence was high. Average pneumococcal DNA load in LytA-positive day-care samples was 6.5 x 10(6) copies/mu l (95%Cl = 3.9-9.2 x 10(6), median = 3.5 x 10(5)); DNA load was positively associated with signs of common cold and negatively with previous antibiotic use. Culture based frequency of 13 pneumococcal vaccine (PCV) serotypes was 5.4% in DCC and 7.7% in AOM, with 19F and 14 being most frequent, and frequencies below 0.5% for serotypes 3, 6A, 19A in both populations. Predominant non-PCV serotypes were 23B and 23A in day-care and 11A in infants with AOM. In day-care, resistance to penicillin was rare (<0.5%) and absent against levofloxacin; 32.7% and 16.9% isolates were cotrimoxazole- and erythromycin-resistant respectively. Conclusion: Four years after PCV13 introduction in the vaccination programme, PCV13 serotype carriage was rare in infants throughout Belgium and penicillin resistance was rare. Continued surveillance in the context of a PCV programme switch is necessary.
Keywords
S. pneumoniae, Nasopharyngeal carriage, Day-care, Otitis media, Infants, ACUTE OTITIS-MEDIA, ATTENDING DAY-CARE, STREPTOCOCCUS-PNEUMONIAE, HAEMOPHILUS-INFLUENZAE, ANTIBIOTIC-RESISTANCE, SEROTYPE DISTRIBUTION, CHILDREN, DISEASE, PATHOGENS, BACTERIAL

Downloads

  • 2018 Vaccine Wouters Pneumo carriage infants 1-s2.0-S0264410X1731616X-main.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 453.40 KB

Citation

Please use this url to cite or link to this publication:

MLA
Wouters, Ine, Liesbet Van Heirstraeten, Stefanie Desmet, et al. “Nasopharyngeal  S. Pneumoniae Carriage and Density in Belgian Infants After 9 Years of Pneumococcal Conjugate Vaccine Programme.” VACCINE 36.1 (2018): 15–22. Print.
APA
Wouters, I., Van Heirstraeten, L., Desmet, S., Blaizot, S., Verhaegen, J., Goossens, H., Van Damme, P., et al. (2018). Nasopharyngeal  S. pneumoniae carriage and density in Belgian infants after 9 years of pneumococcal conjugate vaccine programme. VACCINE, 36(1), 15–22.
Chicago author-date
Wouters, Ine, Liesbet Van Heirstraeten, Stefanie Desmet, Stéphanie Blaizot, Jan Verhaegen, Herman Goossens, Pierre Van Damme, et al. 2018. “Nasopharyngeal  S. Pneumoniae Carriage and Density in Belgian Infants After 9 Years of Pneumococcal Conjugate Vaccine Programme.” Vaccine 36 (1): 15–22.
Chicago author-date (all authors)
Wouters, Ine, Liesbet Van Heirstraeten, Stefanie Desmet, Stéphanie Blaizot, Jan Verhaegen, Herman Goossens, Pierre Van Damme, Surbhi Malhotra-Kumar, Heidi Theeten, the NPcarriage study group, and Koen Van Herck. 2018. “Nasopharyngeal  S. Pneumoniae Carriage and Density in Belgian Infants After 9 Years of Pneumococcal Conjugate Vaccine Programme.” Vaccine 36 (1): 15–22.
Vancouver
1.
Wouters I, Van Heirstraeten L, Desmet S, Blaizot S, Verhaegen J, Goossens H, et al. Nasopharyngeal  S. pneumoniae carriage and density in Belgian infants after 9 years of pneumococcal conjugate vaccine programme. VACCINE. 2018;36(1):15–22.
IEEE
[1]
I. Wouters et al., “Nasopharyngeal  S. pneumoniae  carriage and density in Belgian infants after 9 years of pneumococcal conjugate vaccine programme,” VACCINE, vol. 36, no. 1, pp. 15–22, 2018.
@article{8547548,
  abstract     = {Background: In Belgium, the infant pneumococcal conjugate vaccine (PCV) programme changed from PCV7 (2007-2011) to PCV13 (2011-2015) and to PCV10 (2015-2016). A 3-year nasopharyngeal carriage study was initiated during the programme switch in 2016. Main objective of the year 1 assessment was to obtain a baseline measurement of pneumococcal carriage prevalence, carriage density, serotype distribution and antibiotic resistance. 
Materials/methods: Two infant populations aged 6-30 months and without use of antibiotics in the seven days prior to sampling were approached: (1) attending one of 85 randomly selected day-care centres (DCC); (2) presenting with AOM at study-trained general practitioners and paediatricians. Demographic and clinical characteristics were documented and a single nasopharyngeal swab was taken. S. pneumoniae were cultured, screened for antibiotic resistance and serotyped, and quantitative Taqman real-time PCR (qRT-PCR) targeting LytA was performed. 
Results: Culture-based (DCC: 462/760; 60.8%- AOM: 27/39; 69.2%) and LytA-based (DCC: 603/753; 80.1% - AOM: 32/39; 82.1%) carriage prevalence was high. Average pneumococcal DNA load in LytA-positive day-care samples was 6.5 x 10(6) copies/mu l (95%Cl = 3.9-9.2 x 10(6), median = 3.5 x 10(5)); DNA load was positively associated with signs of common cold and negatively with previous antibiotic use. Culture based frequency of 13 pneumococcal vaccine (PCV) serotypes was 5.4% in DCC and 7.7% in AOM, with 19F and 14 being most frequent, and frequencies below 0.5% for serotypes 3, 6A, 19A in both populations. Predominant non-PCV serotypes were 23B and 23A in day-care and 11A in infants with AOM. In day-care, resistance to penicillin was rare (<0.5%) and absent against levofloxacin; 32.7% and 16.9% isolates were cotrimoxazole- and erythromycin-resistant respectively. 
Conclusion: Four years after PCV13 introduction in the vaccination programme, PCV13 serotype carriage was rare in infants throughout Belgium and penicillin resistance was rare. Continued surveillance in the context of a PCV programme switch is necessary.},
  author       = {Wouters, Ine and Van Heirstraeten, Liesbet and Desmet, Stefanie and Blaizot, Stéphanie and Verhaegen, Jan and Goossens, Herman and Van Damme, Pierre and Malhotra-Kumar, Surbhi and Theeten, Heidi and NPcarriage study group, the and Van Herck, Koen},
  issn         = {0264-410X},
  journal      = {VACCINE},
  keywords     = {S. pneumoniae,Nasopharyngeal carriage,Day-care,Otitis media,Infants,ACUTE OTITIS-MEDIA,ATTENDING DAY-CARE,STREPTOCOCCUS-PNEUMONIAE,HAEMOPHILUS-INFLUENZAE,ANTIBIOTIC-RESISTANCE,SEROTYPE DISTRIBUTION,CHILDREN,DISEASE,PATHOGENS,BACTERIAL},
  language     = {eng},
  number       = {1},
  pages        = {15--22},
  title        = {Nasopharyngeal  S. pneumoniae  carriage and density in Belgian infants after 9 years of pneumococcal conjugate vaccine programme},
  url          = {http://dx.doi.org/10.1016/j.vaccine.2017.11.052},
  volume       = {36},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: