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Metabolic profile of the masked mycotoxin T-2 toxin-3-glucoside in rats (in vitro and in vivo) and humans (in vitro)

(2017) WORLD MYCOTOXIN JOURNAL. 10(4). p.349-362
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Abstract
The metabolic profile of T-2 toxin-3-glucoside (T2-Glc) in humans and rats was investigated using ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF). When rat and human liver microsomes were incubated with T2-Glc, a total of five metabolites were detected. T2-Glc exposed a higher metabolic stability in rats and human than T-2 toxin (T-2). The metabolism of T2-Glc by the intestinal microbiota of human and rats was also investigated, and three metabolites were observed. T2-Glc was reconverted to T-2 during incubation with fresh faeces. Furthermore, in vivo metabolism of T2-Glc in rats after oral administration was carried out, and three metabolites were detected in rat urine and faeces (T-2, HT-2 toxin and 3'-OH-T2-Glc). In vivo metabolism results indicated that T2-Glc was mainly metabolised in the gastro-intestinal tract with a low absorption level in rats. The results demonstrated that hydroxylation (C-3' and C-4'), hydrolysis (C-4 and C-8) and deconjugation are the main metabolic pathways of T2-Glc in mammals.
Keywords
HYBRID MASS-SPECTROMETRY, NATURALLY CONTAMINATED WHEAT, A, TRICHOTHECENES, TOXIN, DEOXYNIVALENOL, GLUCOSIDE, DERIVATIVES, XENOBIOTICS, ZEARALENONE, MICROSOMES, T-2 toxin, T-2 toxin-3-glucoside, metabolism, liver, UHPLC-QTOF

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Citation

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Chicago
Yang, Shupeng, C Van Poucke, Z Wang, S Zhang, Sarah De Saeger, and Marthe De Boevre. 2017. “Metabolic Profile of the Masked Mycotoxin T-2 Toxin-3-glucoside in Rats (in Vitro and in Vivo) and Humans (in Vitro).” World Mycotoxin Journal 10 (4): 349–362.
APA
Yang, Shupeng, Van Poucke, C., Wang, Z., Zhang, S., De Saeger, S., & De Boevre, M. (2017). Metabolic profile of the masked mycotoxin T-2 toxin-3-glucoside in rats (in vitro and in vivo) and humans (in vitro). WORLD MYCOTOXIN JOURNAL, 10(4), 349–362.
Vancouver
1.
Yang S, Van Poucke C, Wang Z, Zhang S, De Saeger S, De Boevre M. Metabolic profile of the masked mycotoxin T-2 toxin-3-glucoside in rats (in vitro and in vivo) and humans (in vitro). WORLD MYCOTOXIN JOURNAL. 2017;10(4):349–62.
MLA
Yang, Shupeng, C Van Poucke, Z Wang, et al. “Metabolic Profile of the Masked Mycotoxin T-2 Toxin-3-glucoside in Rats (in Vitro and in Vivo) and Humans (in Vitro).” WORLD MYCOTOXIN JOURNAL 10.4 (2017): 349–362. Print.
@article{8547520,
  abstract     = {The metabolic profile of T-2 toxin-3-glucoside (T2-Glc) in humans and rats was investigated using ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF). When rat and human liver microsomes were incubated with T2-Glc, a total of five metabolites were detected. T2-Glc exposed a higher metabolic stability in rats and human than T-2 toxin (T-2). The metabolism of T2-Glc by the intestinal microbiota of human and rats was also investigated, and three metabolites were observed. T2-Glc was reconverted to T-2 during incubation with fresh faeces. Furthermore, in vivo metabolism of T2-Glc in rats after oral administration was carried out, and three metabolites were detected in rat urine and faeces (T-2, HT-2 toxin and 3'-OH-T2-Glc). In vivo metabolism results indicated that T2-Glc was mainly metabolised in the gastro-intestinal tract with a low absorption level in rats. The results demonstrated that hydroxylation (C-3' and C-4'), hydrolysis (C-4 and C-8) and deconjugation are the main metabolic pathways of T2-Glc in mammals.},
  author       = {Yang, Shupeng and Van Poucke, C and Wang, Z and Zhang, S and De Saeger, Sarah and De Boevre, Marthe},
  issn         = {1875-0710},
  journal      = {WORLD MYCOTOXIN JOURNAL},
  keyword      = {HYBRID MASS-SPECTROMETRY,NATURALLY CONTAMINATED WHEAT,A,TRICHOTHECENES,TOXIN,DEOXYNIVALENOL,GLUCOSIDE,DERIVATIVES,XENOBIOTICS,ZEARALENONE,MICROSOMES,T-2 toxin,T-2 toxin-3-glucoside,metabolism,liver,UHPLC-QTOF},
  language     = {eng},
  number       = {4},
  pages        = {349--362},
  title        = {Metabolic profile of the masked mycotoxin T-2 toxin-3-glucoside in rats (in vitro and in vivo) and humans (in vitro)},
  url          = {http://dx.doi.org/10.3920/WMJ2017.2224},
  volume       = {10},
  year         = {2017},
}

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