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Dihydrofolate reductase/thymidylate synthase fine-tunes the folate status and controls redox homeostasis in plants

(2017) PLANT CELL. 29(11). p.2831-2853
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Abstract
Folates (B9 vitamins) are essential cofactors in one-carbon metabolism. Since C1 transfer reactions are involved in synthesis of nucleic acids, proteins, lipids, and other biomolecules, as well as in epigenetic control, folates are vital for all living organisms. This work presents a complete study of a plant DHFR-TS (dihydrofolate reductase-thymidylate synthase) gene family that implements the penultimate step in folate biosynthesis. We demonstrate that one of the DHFR-TS isoforms (DHFRTS3) operates as an inhibitor of its two homologs, thus regulating DHFR and TS activities and, as a consequence, folate abundance. In addition, a novel function of folate metabolism in plants is proposed, i.e., maintenance of the redox balance by contributing to NADPH production through the reaction catalyzed by methylenetetrahydrofolate dehydrogenase, thus allowing plants to cope with oxidative stress.
Keywords
ONE-CARBON METABOLISM, DEPENDENT KINASE INHIBITORS, DNA-DAMAGE CHECKPOINT, ARABIDOPSIS-THALIANA, THYMIDYLATE SYNTHASE, OXIDATIVE STRESS, REDUCTASE GENE, FOLIC-ACID, FOLYLPOLYGLUTAMATE SYNTHETASE, FUNCTIONAL-ANALYSIS

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Citation

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Chicago
Gorelova, Vera, Jolien De Lepeleire, Jeroen Van Daele, Dick Pluim, Coline Mei, Ann Cuypers, Olivier Leroux, et al. 2017. “Dihydrofolate Reductase/thymidylate Synthase Fine-tunes the Folate Status and Controls Redox Homeostasis in Plants.” Plant Cell 29 (11): 2831–2853.
APA
Gorelova, V., De Lepeleire, J., Van Daele, J., Pluim, D., Mei, C., Cuypers, A., Leroux, O., et al. (2017). Dihydrofolate reductase/thymidylate synthase fine-tunes the folate status and controls redox homeostasis in plants. PLANT CELL, 29(11), 2831–2853.
Vancouver
1.
Gorelova V, De Lepeleire J, Van Daele J, Pluim D, Mei C, Cuypers A, et al. Dihydrofolate reductase/thymidylate synthase fine-tunes the folate status and controls redox homeostasis in plants. PLANT CELL. 2017;29(11):2831–53.
MLA
Gorelova, Vera, Jolien De Lepeleire, Jeroen Van Daele, et al. “Dihydrofolate Reductase/thymidylate Synthase Fine-tunes the Folate Status and Controls Redox Homeostasis in Plants.” PLANT CELL 29.11 (2017): 2831–2853. Print.
@article{8547260,
  abstract     = {Folates (B9 vitamins) are essential cofactors in one-carbon metabolism. Since C1 transfer reactions are involved in synthesis of nucleic acids, proteins, lipids, and other biomolecules, as well as in epigenetic control, folates are vital for all living organisms. This work presents a complete study of a plant DHFR-TS (dihydrofolate reductase-thymidylate synthase) gene family that implements the penultimate step in folate biosynthesis. We demonstrate that one of the DHFR-TS isoforms (DHFRTS3) operates as an inhibitor of its two homologs, thus regulating DHFR and TS activities and, as a consequence, folate abundance. In addition, a novel function of folate metabolism in plants is proposed, i.e., maintenance of the redox balance by contributing to NADPH production through the reaction catalyzed by methylenetetrahydrofolate dehydrogenase, thus allowing plants to cope with oxidative stress.},
  author       = {Gorelova, Vera and De Lepeleire, Jolien and Van Daele, Jeroen and Pluim, Dick and Mei, Coline and Cuypers, Ann and Leroux, Olivier and Rebeille, Fabrice and Schellens, Jan H and Blancquaert, Dieter and Stove, Christophe and Van Der Straeten, Dominique},
  issn         = { 1040-4651},
  journal      = {PLANT CELL},
  keyword      = {ONE-CARBON METABOLISM,DEPENDENT KINASE INHIBITORS,DNA-DAMAGE CHECKPOINT,ARABIDOPSIS-THALIANA,THYMIDYLATE SYNTHASE,OXIDATIVE STRESS,REDUCTASE GENE,FOLIC-ACID,FOLYLPOLYGLUTAMATE SYNTHETASE,FUNCTIONAL-ANALYSIS},
  language     = {eng},
  number       = {11},
  pages        = {2831--2853},
  title        = {Dihydrofolate reductase/thymidylate synthase fine-tunes the folate status and controls redox homeostasis in plants},
  url          = {http://dx.doi.org/10.1105/tpc.17.00433},
  volume       = {29},
  year         = {2017},
}

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