Ghent University Academic Bibliography

Advanced

Dihydrofolate reductase/thymidylate synthase fine-tunes the folate status and controls redox homeostasis in plants

Vera Gorelova UGent, Jolien De Lepeleire UGent, Jeroen Van Daele, Dick Pluim, Coline Mei, Ann Cuypers, Olivier Leroux UGent, Fabrice Rebeille, Jan H Schellens, Dieter Blancquaert, et al. (2017) PLANT CELL. 29(11). p.2831-2853
abstract
Folates (B9 vitamins) are essential cofactors in one-carbon metabolism. Since C1 transfer reactions are involved in synthesis of nucleic acids, proteins, lipids, and other biomolecules, as well as in epigenetic control, folates are vital for all living organisms. This work presents a complete study of a plant DHFR-TS (dihydrofolate reductase-thymidylate synthase) gene family that implements the penultimate step in folate biosynthesis. We demonstrate that one of the DHFR-TS isoforms (DHFRTS3) operates as an inhibitor of its two homologs, thus regulating DHFR and TS activities and, as a consequence, folate abundance. In addition, a novel function of folate metabolism in plants is proposed, i.e., maintenance of the redox balance by contributing to NADPH production through the reaction catalyzed by methylenetetrahydrofolate dehydrogenase, thus allowing plants to cope with oxidative stress.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ONE-CARBON METABOLISM, DEPENDENT KINASE INHIBITORS, DNA-DAMAGE CHECKPOINT, ARABIDOPSIS-THALIANA, THYMIDYLATE SYNTHASE, OXIDATIVE STRESS, REDUCTASE GENE, FOLIC-ACID, FOLYLPOLYGLUTAMATE SYNTHETASE, FUNCTIONAL-ANALYSIS
journal title
PLANT CELL
Plant Cell
volume
29
issue
11
pages
2831 - 2853
Web of Science id
000417643700015
ISSN
1040-4651
1532-298X
DOI
10.1105/tpc.17.00433
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8547260
handle
http://hdl.handle.net/1854/LU-8547260
date created
2018-02-01 10:42:21
date last changed
2018-02-13 09:37:22
@article{8547260,
  abstract     = {Folates (B9 vitamins) are essential cofactors in one-carbon metabolism. Since C1 transfer reactions are involved in synthesis of nucleic acids, proteins, lipids, and other biomolecules, as well as in epigenetic control, folates are vital for all living organisms. This work presents a complete study of a plant DHFR-TS (dihydrofolate reductase-thymidylate synthase) gene family that implements the penultimate step in folate biosynthesis. We demonstrate that one of the DHFR-TS isoforms (DHFRTS3) operates as an inhibitor of its two homologs, thus regulating DHFR and TS activities and, as a consequence, folate abundance. In addition, a novel function of folate metabolism in plants is proposed, i.e., maintenance of the redox balance by contributing to NADPH production through the reaction catalyzed by methylenetetrahydrofolate dehydrogenase, thus allowing plants to cope with oxidative stress.},
  author       = {Gorelova, Vera and De Lepeleire, Jolien and Van Daele, Jeroen and Pluim, Dick and Mei, Coline and Cuypers, Ann and Leroux, Olivier and Rebeille, Fabrice and Schellens, Jan H and Blancquaert, Dieter and Stove, Christophe and Van Der Straeten, Dominique},
  issn         = { 1040-4651},
  journal      = {PLANT CELL},
  keyword      = {ONE-CARBON METABOLISM,DEPENDENT KINASE INHIBITORS,DNA-DAMAGE CHECKPOINT,ARABIDOPSIS-THALIANA,THYMIDYLATE SYNTHASE,OXIDATIVE STRESS,REDUCTASE GENE,FOLIC-ACID,FOLYLPOLYGLUTAMATE SYNTHETASE,FUNCTIONAL-ANALYSIS},
  language     = {eng},
  number       = {11},
  pages        = {2831--2853},
  title        = {Dihydrofolate reductase/thymidylate synthase fine-tunes the folate status and controls redox homeostasis in plants},
  url          = {http://dx.doi.org/10.1105/tpc.17.00433},
  volume       = {29},
  year         = {2017},
}

Chicago
Gorelova, Vera, Jolien De Lepeleire, Jeroen Van Daele, Dick Pluim, Coline Mei, Ann Cuypers, Olivier Leroux, et al. 2017. “Dihydrofolate Reductase/thymidylate Synthase Fine-tunes the Folate Status and Controls Redox Homeostasis in Plants.” Plant Cell 29 (11): 2831–2853.
APA
Gorelova, V., De Lepeleire, J., Van Daele, J., Pluim, D., Mei, C., Cuypers, A., Leroux, O., et al. (2017). Dihydrofolate reductase/thymidylate synthase fine-tunes the folate status and controls redox homeostasis in plants. PLANT CELL, 29(11), 2831–2853.
Vancouver
1.
Gorelova V, De Lepeleire J, Van Daele J, Pluim D, Mei C, Cuypers A, et al. Dihydrofolate reductase/thymidylate synthase fine-tunes the folate status and controls redox homeostasis in plants. PLANT CELL. 2017;29(11):2831–53.
MLA
Gorelova, Vera, Jolien De Lepeleire, Jeroen Van Daele, et al. “Dihydrofolate Reductase/thymidylate Synthase Fine-tunes the Folate Status and Controls Redox Homeostasis in Plants.” PLANT CELL 29.11 (2017): 2831–2853. Print.