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Nanoscopic X-ray fluorescence imaging and quantification of intracellular key-elements in cryofrozen Friedreich's ataxia fibroblasts

Björn De Samber UGent, Eline Meul, Brecht Laforce UGent, Boel De Paepe UGent, Joél Smet UGent, Michiel De Bruyne UGent, Riet De Rycke UGent, Sylvain Bohic, Peter Cloetens, Rudy Van Coster UGent, et al. (2018) PLOS ONE. 13(1).
abstract
Synchrotron radiation based nanoscopic X-ray fluorescence (SR nano-XRF) analysis can visualize trace level elemental distribution in a fully quantitative manner within single cells. However, in-air XRF analysis requires chemical fixation modifying the cell's chemical composition. Here, we describe first nanoscopic XRF analysis upon cryogenically frozen (-150 degrees C) fibroblasts at the ID16A-NI 'Nano-imaging' end-station located at the European Synchrotron Radiation Facility (ESRF) in Grenoble (France). Fibroblast cells were obtained from skin biopsies from control and Friedreich's ataxia (FRDA) patients. FRDA is an autosomal recessive disorder with dysregulation of iron metabolism as a key feature. By means of the X-ray Fundamental Parameter (FP) method, including absorption correction of the ice layer deposited onto the fibroblasts, background-corrected mass fraction elemental maps of P, S, Cl, K, Ca, Fe and Zn of entire cryofrozen human fibroblasts were obtained. Despite the presence of diffracting microcrystals in the vitreous ice matrix and minor sample radiation damage effects, clusters of iron-rich hot-spots with similar mass fractions were found in the cytoplasm of both control and FRDA fibroblasts. Interestingly, no significant difference in the mean iron concentration was found in the cytoplasm of FRDA fibroblasts, but a significant decrease in zinc concentration. This finding might underscore metal dysregulation, beyond iron, in cells derived from FRDA patients. In conclusion, although currently having slightly increased limits of detection (LODs) compared to non-cryogenic mode, SR based nanoscopic XRF under cryogenic sample conditions largely obliterates the debate on chemical sample preservation and provides a unique tool for trace level elemental imaging in single cells close to their native state with a superior spatial resolution of 20 nm.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
YEAST FRATAXIN, OXIDATIVE STRESS, IRON, ZINC, CARDIOMYOPATHY, REDISTRIBUTION, DEFICIENCY, LIBRARY, PROTEIN, COPPER
journal title
PLOS ONE
PLoS One
volume
13
issue
1
article number
e0190495
pages
24 pages
Web of Science type
Article
Web of Science id
000422653800024
ISSN
1932-6203
DOI
10.1371/journal.pone.0190495
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8547221
handle
http://hdl.handle.net/1854/LU-8547221
date created
2018-02-01 10:21:21
date last changed
2018-02-15 15:18:54
@article{8547221,
  abstract     = {Synchrotron radiation based nanoscopic X-ray fluorescence (SR nano-XRF) analysis can visualize trace level elemental distribution in a fully quantitative manner within single cells. However, in-air XRF analysis requires chemical fixation modifying the cell's chemical composition. Here, we describe first nanoscopic XRF analysis upon cryogenically frozen (-150 degrees C) fibroblasts at the ID16A-NI 'Nano-imaging' end-station located at the European Synchrotron Radiation Facility (ESRF) in Grenoble (France). Fibroblast cells were obtained from skin biopsies from control and Friedreich's ataxia (FRDA) patients. FRDA is an autosomal recessive disorder with dysregulation of iron metabolism as a key feature. By means of the X-ray Fundamental Parameter (FP) method, including absorption correction of the ice layer deposited onto the fibroblasts, background-corrected mass fraction elemental maps of P, S, Cl, K, Ca, Fe and Zn of entire cryofrozen human fibroblasts were obtained. Despite the presence of diffracting microcrystals in the vitreous ice matrix and minor sample radiation damage effects, clusters of iron-rich hot-spots with similar mass fractions were found in the cytoplasm of both control and FRDA fibroblasts. Interestingly, no significant difference in the mean iron concentration was found in the cytoplasm of FRDA fibroblasts, but a significant decrease in zinc concentration. This finding might underscore metal dysregulation, beyond iron, in cells derived from FRDA patients. In conclusion, although currently having slightly increased limits of detection (LODs) compared to non-cryogenic mode, SR based nanoscopic XRF under cryogenic sample conditions largely obliterates the debate on chemical sample preservation and provides a unique tool for trace level elemental imaging in single cells close to their native state with a superior spatial resolution of 20 nm.},
  articleno    = {e0190495},
  author       = {De Samber, Bj{\"o}rn and Meul, Eline and Laforce, Brecht and De Paepe, Boel and Smet, Jo{\'e}l and De Bruyne, Michiel and De Rycke, Riet and Bohic, Sylvain and Cloetens, Peter and Van Coster, Rudy and Vandenabeele, Peter and Vanden Berghe, Tom},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {YEAST FRATAXIN,OXIDATIVE STRESS,IRON,ZINC,CARDIOMYOPATHY,REDISTRIBUTION,DEFICIENCY,LIBRARY,PROTEIN,COPPER},
  language     = {eng},
  number       = {1},
  pages        = {24},
  title        = {Nanoscopic X-ray fluorescence imaging and quantification of intracellular key-elements in cryofrozen Friedreich's ataxia fibroblasts},
  url          = {http://dx.doi.org/10.1371/journal.pone.0190495},
  volume       = {13},
  year         = {2018},
}

Chicago
De Samber, Björn, Eline Meul, Brecht Laforce, Boel De Paepe, Joél Smet, Michiel De Bruyne, Riet De Rycke, et al. 2018. “Nanoscopic X-ray Fluorescence Imaging and Quantification of Intracellular Key-elements in Cryofrozen Friedreich’s Ataxia Fibroblasts.” Plos One 13 (1).
APA
De Samber, B., Meul, E., Laforce, B., De Paepe, B., Smet, J., De Bruyne, M., De Rycke, R., et al. (2018). Nanoscopic X-ray fluorescence imaging and quantification of intracellular key-elements in cryofrozen Friedreich’s ataxia fibroblasts. PLOS ONE, 13(1).
Vancouver
1.
De Samber B, Meul E, Laforce B, De Paepe B, Smet J, De Bruyne M, et al. Nanoscopic X-ray fluorescence imaging and quantification of intracellular key-elements in cryofrozen Friedreich’s ataxia fibroblasts. PLOS ONE. 2018;13(1).
MLA
De Samber, Björn, Eline Meul, Brecht Laforce, et al. “Nanoscopic X-ray Fluorescence Imaging and Quantification of Intracellular Key-elements in Cryofrozen Friedreich’s Ataxia Fibroblasts.” PLOS ONE 13.1 (2018): n. pag. Print.