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Folate biofortification of potato by tuber-specific expression of four folate biosynthesis genes

(2018) MOLECULAR PLANT. 11(1). p.175-188
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Abstract
Insufficient dietary intake of micronutrients, known as "hidden hunger'', is a devastating global burden, affecting two billion people. Deficiency of folates (vitamin B9), which are known to play a central role in C-1 metabolism, causes birth defects in at least a quarter million people annually. Biofortification to enhance the level of naturally occurring folates in crop plants, proves to be an efficient and cost-effective tool in fighting folate deficiency. Previously, introduction of folate biosynthesis genes GTPCHI and ADCS, proven to be a successful biofortification strategy in rice and tomato, turned out to be insufficient to adequately increase folate levels in potato tubers. Here, we provide a proof of concept that additional introduction of HPPK/DHPS and/or FPGS, downstream genes in mitochondrial folate biosynthesis, enables augmentation of folates to satisfactory levels (12-fold) and ensures folate stability upon long-term storage of tubers. In conclusion, this engineering strategy can serve as a model in the creation of folate-accumulating potato cultivars, readily applicable in potato-consuming populations suffering from folate deficiency.
Keywords
folate, vitamin B9, malnutrition, biofortification, potato, metabolic engineering, FOLIC-ACID, MICRONUTRIENT DEFICIENCIES, PLANT TRANSFORMATION, ARABIDOPSIS-THALIANA, DNA METHYLATION, TOMATO FRUIT, CROP PLANTS, RESISTANCE, RICE, HOMOCYSTEINE

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Chicago
De Lepeleire, Jolien, Simon Strobbe, Jana Verstraete, Dieter Blancquaert, Lars Ambach, Richard GF Visser, Christophe Stove, and Dominique Van Der Straeten. 2018. “Folate Biofortification of Potato by Tuber-specific Expression of Four Folate Biosynthesis Genes.” Molecular Plant 11 (1): 175–188.
APA
De Lepeleire, Jolien, Strobbe, S., Verstraete, J., Blancquaert, D., Ambach, L., Visser, R. G., Stove, C., et al. (2018). Folate biofortification of potato by tuber-specific expression of four folate biosynthesis genes. MOLECULAR PLANT, 11(1), 175–188.
Vancouver
1.
De Lepeleire J, Strobbe S, Verstraete J, Blancquaert D, Ambach L, Visser RG, et al. Folate biofortification of potato by tuber-specific expression of four folate biosynthesis genes. MOLECULAR PLANT. 2018;11(1):175–88.
MLA
De Lepeleire, Jolien, Simon Strobbe, Jana Verstraete, et al. “Folate Biofortification of Potato by Tuber-specific Expression of Four Folate Biosynthesis Genes.” MOLECULAR PLANT 11.1 (2018): 175–188. Print.
@article{8547191,
  abstract     = {Insufficient dietary intake of micronutrients, known as {\textacutedbl}hidden hunger'', is a devastating global burden, affecting two billion people. Deficiency of folates (vitamin B9), which are known to play a central role in C-1 metabolism, causes birth defects in at least a quarter million people annually. Biofortification to enhance the level of naturally occurring folates in crop plants, proves to be an efficient and cost-effective tool in fighting folate deficiency. Previously, introduction of folate biosynthesis genes GTPCHI and ADCS, proven to be a successful biofortification strategy in rice and tomato, turned out to be insufficient to adequately increase folate levels in potato tubers. Here, we provide a proof of concept that additional introduction of HPPK/DHPS and/or FPGS, downstream genes in mitochondrial folate biosynthesis, enables augmentation of folates to satisfactory levels (12-fold) and ensures folate stability upon long-term storage of tubers. In conclusion, this engineering strategy can serve as a model in the creation of folate-accumulating potato cultivars, readily applicable in potato-consuming populations suffering from folate deficiency.},
  author       = {De Lepeleire, Jolien and Strobbe, Simon and Verstraete, Jana and Blancquaert, Dieter and Ambach, Lars and Visser, Richard GF and Stove, Christophe and Van Der Straeten, Dominique},
  issn         = {1674-2052},
  journal      = {MOLECULAR PLANT},
  language     = {eng},
  number       = {1},
  pages        = {175--188},
  title        = {Folate biofortification of potato by tuber-specific expression of four folate biosynthesis genes},
  url          = {http://dx.doi.org/10.1016/j.molp.2017.12.008},
  volume       = {11},
  year         = {2018},
}

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