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Nanobody technology : a versatile toolkit for microscopic imaging, protein-protein interaction analysis, and protein function exploration

Els Beghein (UGent) and Jan Gettemans (UGent)
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Abstract
Over the last two decades, nanobodies or single-domain antibodies have found their way in research, diagnostics, and therapy. These antigen-binding fragments, derived from Camelid heavy chain only antibodies, possess remarkable characteristics that favor their use over conventional antibodies or fragments thereof, in selected areas of research. In this review, we assess the current status of nanobodies as research tools in diverse aspects of fundamental research. We discuss the use of nanobodies as detection reagents in fluorescence microscopy and focus on recent advances in super-resolution microscopy. Second, application of nanobody technology in investigating protein-protein interactions is reviewed, with emphasis on possible uses in mass spectrometry. Finally, we discuss the potential value of nanobodies in studying protein function, and we focus on their recently reported application in targeted protein degradation. Throughout the review, we highlight state-of-the-art engineering strategies that could expand nanobody versatility and we suggest future applications of the technology in the selected areas of fundamental research.
Keywords
nanobody, VHH, single-domain antibody, engineering, super-resolution microscopy, protein-protein interactions, targeted protein degradation, fundamental research, EXCHANGE MASS-SPECTROMETRY, DOMAIN ANTIBODY-FRAGMENTS, TOXINS ENZYMATIC SUBUNIT, HEAVY-CHAIN ANTIBODIES, MAMMALIAN-CELLS, LIVING CELLS, SUPERRESOLUTION MICROSCOPY, FLUORESCENCE MICROSCOPY, MONOCLONAL-ANTIBODY, STRUCTURAL-ANALYSIS

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MLA
Beghein, Els, and Jan Gettemans. “Nanobody Technology : a Versatile Toolkit for Microscopic Imaging, Protein-protein Interaction Analysis, and Protein Function Exploration.” FRONTIERS IN IMMUNOLOGY 8 (2017): n. pag. Print.
APA
Beghein, E., & Gettemans, J. (2017). Nanobody technology : a versatile toolkit for microscopic imaging, protein-protein interaction analysis, and protein function exploration. FRONTIERS IN IMMUNOLOGY, 8.
Chicago author-date
Beghein, Els, and Jan Gettemans. 2017. “Nanobody Technology : a Versatile Toolkit for Microscopic Imaging, Protein-protein Interaction Analysis, and Protein Function Exploration.” Frontiers in Immunology 8.
Chicago author-date (all authors)
Beghein, Els, and Jan Gettemans. 2017. “Nanobody Technology : a Versatile Toolkit for Microscopic Imaging, Protein-protein Interaction Analysis, and Protein Function Exploration.” Frontiers in Immunology 8.
Vancouver
1.
Beghein E, Gettemans J. Nanobody technology : a versatile toolkit for microscopic imaging, protein-protein interaction analysis, and protein function exploration. FRONTIERS IN IMMUNOLOGY. 2017;8.
IEEE
[1]
E. Beghein and J. Gettemans, “Nanobody technology : a versatile toolkit for microscopic imaging, protein-protein interaction analysis, and protein function exploration,” FRONTIERS IN IMMUNOLOGY, vol. 8, 2017.
@article{8547150,
  abstract     = {Over the last two decades, nanobodies or single-domain antibodies have found their way in research, diagnostics, and therapy. These antigen-binding fragments, derived from Camelid heavy chain only antibodies, possess remarkable characteristics that favor their use over conventional antibodies or fragments thereof, in selected areas of research. In this review, we assess the current status of nanobodies as research tools in diverse aspects of fundamental research. We discuss the use of nanobodies as detection reagents in fluorescence microscopy and focus on recent advances in super-resolution microscopy. Second, application of nanobody technology in investigating protein-protein interactions is reviewed, with emphasis on possible uses in mass spectrometry. Finally, we discuss the potential value of nanobodies in studying protein function, and we focus on their recently reported application in targeted protein degradation. Throughout the review, we highlight state-of-the-art engineering strategies that could expand nanobody versatility and we suggest future applications of the technology in the selected areas of fundamental research.},
  articleno    = {771},
  author       = {Beghein, Els and Gettemans, Jan},
  issn         = {1664-3224},
  journal      = {FRONTIERS IN IMMUNOLOGY},
  keywords     = {nanobody,VHH,single-domain antibody,engineering,super-resolution microscopy,protein-protein interactions,targeted protein degradation,fundamental research,EXCHANGE MASS-SPECTROMETRY,DOMAIN ANTIBODY-FRAGMENTS,TOXINS ENZYMATIC SUBUNIT,HEAVY-CHAIN ANTIBODIES,MAMMALIAN-CELLS,LIVING CELLS,SUPERRESOLUTION MICROSCOPY,FLUORESCENCE MICROSCOPY,MONOCLONAL-ANTIBODY,STRUCTURAL-ANALYSIS},
  language     = {eng},
  pages        = {14},
  title        = {Nanobody technology : a versatile toolkit for microscopic imaging, protein-protein interaction analysis, and protein function exploration},
  url          = {http://dx.doi.org/10.3389/fimmu.2017.00771},
  volume       = {8},
  year         = {2017},
}

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