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Genetic divergence and chemotype diversity in the fusarium head blight pathogen Fusarium poae

Adriaan Vanheule, Marthe De Boevre UGent, Antonio Moretti, Jonathan Scauflaire, Francoise Munaut, Sarah De Saeger UGent, Boris Bekaert UGent, Geert Haesaert UGent, Cees Waalwijk, Theo van der Lee, et al. (2017) TOXINS. 9(9).
abstract
Fusarium head blight is a disease caused by a complex of Fusarium species. F. poae is omnipresent throughout Europe in spite of its low virulence. In this study, we assessed a geographically diverse collection of F. poae isolates for its genetic diversity using AFLP (Amplified Fragment Length Polymorphism). Furthermore, studying the mating type locus and chromosomal insertions, we identified hallmarks of both sexual recombination and clonal spread of successful genotypes in the population. Despite the large genetic variation found, all F. poae isolates possess the nivalenol chemotype based on Tri7 sequence analysis. Nevertheless, Tri gene clusters showed two layers of genetic variability. Firstly, the Tri1 locus was highly variable with mostly synonymous mutations and mutations in introns pointing to a strong purifying selection pressure. Secondly, in a subset of isolates, the main trichothecene gene cluster was invaded by a transposable element between Tri5 and Tri6. To investigate the impact of these variations on the phenotypic chemotype, mycotoxin production was assessed on artificial medium. Complex blends of type A and type B trichothecenes were produced but neither genetic variability in the Tri genes nor variability in the genome or geography accounted for the divergence in trichothecene production. In view of its complex chemotype, it will be of utmost interest to uncover the role of trichothecenes in virulence, spread and survival of F. poae.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IN-VITRO, VEGETATIVE COMPATIBILITY, TRICHOTHECENE MYCOTOXIN, POPULATION-GENETICS, PCR ASSAY, WHEAT, GRAMINEARUM, POLYMORPHISM, SEQUENCES, EVOLUTION, Fusarium, AFLP, trichothecenes, transposable element, mating type, meiosis
journal title
TOXINS
Toxins
volume
9
issue
9
article number
255
pages
19 pages
Web of Science type
Article
Web of Science id
000411961700001
ISSN
2072-6651
DOI
10.3390/toxins9090255
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8546905
handle
http://hdl.handle.net/1854/LU-8546905
date created
2018-01-31 08:09:18
date last changed
2018-06-21 12:44:58
@article{8546905,
  abstract     = {Fusarium head blight is a disease caused by a complex of Fusarium species. F. poae is omnipresent throughout Europe in spite of its low virulence. In this study, we assessed a geographically diverse collection of F. poae isolates for its genetic diversity using AFLP (Amplified Fragment Length Polymorphism). Furthermore, studying the mating type locus and chromosomal insertions, we identified hallmarks of both sexual recombination and clonal spread of successful genotypes in the population. Despite the large genetic variation found, all F. poae isolates possess the nivalenol chemotype based on Tri7 sequence analysis. Nevertheless, Tri gene clusters showed two layers of genetic variability. Firstly, the Tri1 locus was highly variable with mostly synonymous mutations and mutations in introns pointing to a strong purifying selection pressure. Secondly, in a subset of isolates, the main trichothecene gene cluster was invaded by a transposable element between Tri5 and Tri6. To investigate the impact of these variations on the phenotypic chemotype, mycotoxin production was assessed on artificial medium. Complex blends of type A and type B trichothecenes were produced but neither genetic variability in the Tri genes nor variability in the genome or geography accounted for the divergence in trichothecene production. In view of its complex chemotype, it will be of utmost interest to uncover the role of trichothecenes in virulence, spread and survival of F. poae.},
  articleno    = {255},
  author       = {Vanheule, Adriaan and De Boevre, Marthe and Moretti, Antonio and Scauflaire, Jonathan and Munaut, Francoise and De Saeger, Sarah and Bekaert, Boris and Haesaert, Geert and Waalwijk, Cees and van der Lee, Theo and Audenaert, Kris},
  issn         = {2072-6651},
  journal      = {TOXINS},
  keyword      = {IN-VITRO,VEGETATIVE COMPATIBILITY,TRICHOTHECENE MYCOTOXIN,POPULATION-GENETICS,PCR ASSAY,WHEAT,GRAMINEARUM,POLYMORPHISM,SEQUENCES,EVOLUTION,Fusarium,AFLP,trichothecenes,transposable element,mating type,meiosis},
  language     = {eng},
  number       = {9},
  pages        = {19},
  title        = {Genetic divergence and chemotype diversity in the fusarium head blight pathogen Fusarium poae},
  url          = {http://dx.doi.org/10.3390/toxins9090255},
  volume       = {9},
  year         = {2017},
}

Chicago
Vanheule, Adriaan, Marthe De Boevre, Antonio Moretti, Jonathan Scauflaire, Francoise Munaut, Sarah De Saeger, Boris Bekaert, et al. 2017. “Genetic Divergence and Chemotype Diversity in the Fusarium Head Blight Pathogen Fusarium Poae.” Toxins 9 (9).
APA
Vanheule, A., De Boevre, M., Moretti, A., Scauflaire, J., Munaut, F., De Saeger, S., Bekaert, B., et al. (2017). Genetic divergence and chemotype diversity in the fusarium head blight pathogen Fusarium poae. TOXINS, 9(9).
Vancouver
1.
Vanheule A, De Boevre M, Moretti A, Scauflaire J, Munaut F, De Saeger S, et al. Genetic divergence and chemotype diversity in the fusarium head blight pathogen Fusarium poae. TOXINS. 2017;9(9).
MLA
Vanheule, Adriaan, Marthe De Boevre, Antonio Moretti, et al. “Genetic Divergence and Chemotype Diversity in the Fusarium Head Blight Pathogen Fusarium Poae.” TOXINS 9.9 (2017): n. pag. Print.