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Mechanisms of resistance in the rice cultivar Manikpukha to the rice stem nematode Ditylenchus angustus

Shakhina Khanam (UGent) , Lander Bauters (UGent) , Richard Raj Singh (UGent) , Ruben Verbeek (UGent) , Ashley Haeck (UGent) , Saeed MD Sultan, Kristof Demeestere (UGent) , Tina Kyndt (UGent) and Godelieve Gheysen (UGent)
(2018) MOLECULAR PLANT PATHOLOGY. 19(6). p.1391-1402
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Abstract
The incompatible interaction between the rice cultivar Manikpukha and the rice stem nematode Ditylenchus angustus has been reported recently. This research focuses on the underlying mechanisms of resistance in Manikpukha. Invasion, post-infection development and reproduction of D.angustus were compared in compatible and incompatible interactions to identify the stage inwhich resistance occurs. The results indicate that resistance in Manikpukha is associated with reduced development and reproduction, implying that resistance acts post-invasion. We studied the possible involvement of three classical defence hormones, salicylic acid (SA), jasmonic acid (JA) and ethylene (ET), in response to infection in a compatible interaction using biosynthesis/signalling-deficient transgenic rice lines. All three hormones appear to have an influence on the basal defence of Nipponbare against the stem nematode. Although hormone application increases basal defences, expression studies and hormone analyses after nematode infection in Manikpukha did not show a clear involvement of the hormone defense pathways for SA, ET and JA. However, it seems that OsPAL1 plays a pivotal role in resistance, indicating that the phenylpropanoid pathway and its products might be key players in the incompatible interaction. Lignin measurement showed that, although basal levels are similar, Manikpukha had a significantly higher lignin content on nematode infection, whereas it was decreased in the susceptible cultivar. The results presented here show that SA, ET and JA are involved in basal defences, but the resistance of Manikpukha against D.angustus probably relies on products of the phenylpropanoid pathway.
Keywords
defence hormones, Ditylenchus angustus, lignin, Manikpukha, resistance, ROOT-KNOT NEMATODE, PLANT-PARASITIC NEMATODES, RADOPHOLUS-SIMILIS INFECTION, MELOIDOGYNE-GRAMINICOLA, JASMONATE PATHWAY, CYST-NEMATODE, UFRA DISEASE, PHENYLPROPANOID METABOLISM, HYPERSENSITIVE RESPONSE, ARABIDOPSIS-THALIANA

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Chicago
Khanam, Shakhina, Lander Bauters, Richard Raj Singh, Ruben Verbeek, Ashley Haeck, Saeed MD Sultan, Kristof Demeestere, Tina Kyndt, and Godelieve Gheysen. 2018. “Mechanisms of Resistance in the Rice Cultivar Manikpukha to the Rice Stem Nematode Ditylenchus Angustus.” Molecular Plant Pathology 19 (6): 1391–1402.
APA
Khanam, S., Bauters, L., Singh, R. R., Verbeek, R., Haeck, A., Sultan, S. M., Demeestere, K., et al. (2018). Mechanisms of resistance in the rice cultivar Manikpukha to the rice stem nematode Ditylenchus angustus. MOLECULAR PLANT PATHOLOGY, 19(6), 1391–1402.
Vancouver
1.
Khanam S, Bauters L, Singh RR, Verbeek R, Haeck A, Sultan SM, et al. Mechanisms of resistance in the rice cultivar Manikpukha to the rice stem nematode Ditylenchus angustus. MOLECULAR PLANT PATHOLOGY. 2018;19(6):1391–402.
MLA
Khanam, Shakhina, Lander Bauters, Richard Raj Singh, et al. “Mechanisms of Resistance in the Rice Cultivar Manikpukha to the Rice Stem Nematode Ditylenchus Angustus.” MOLECULAR PLANT PATHOLOGY 19.6 (2018): 1391–1402. Print.
@article{8546878,
  abstract     = {The incompatible interaction between the rice cultivar Manikpukha and the rice stem nematode Ditylenchus angustus has been reported recently. This research focuses on the underlying mechanisms of resistance in Manikpukha. Invasion, post-infection development and reproduction of D.angustus were compared in compatible and incompatible interactions to identify the stage inwhich resistance occurs. The results indicate that resistance in Manikpukha is associated with reduced development and reproduction, implying that resistance acts post-invasion. We studied the possible involvement of three classical defence hormones, salicylic acid (SA), jasmonic acid (JA) and ethylene (ET), in response to infection in a compatible interaction using biosynthesis/signalling-deficient transgenic rice lines. All three hormones appear to have an influence on the basal defence of Nipponbare against the stem nematode. Although hormone application increases basal defences, expression studies and hormone analyses after nematode infection in Manikpukha did not show a clear involvement of the hormone defense pathways for SA, ET and JA. However, it seems that OsPAL1 plays a pivotal role in resistance, indicating that the phenylpropanoid pathway and its products might be key players in the incompatible interaction. Lignin measurement showed that, although basal levels are similar, Manikpukha had a significantly higher lignin content on nematode infection, whereas it was decreased in the susceptible cultivar. The results presented here show that SA, ET and JA are involved in basal defences, but the resistance of Manikpukha against D.angustus probably relies on products of the phenylpropanoid pathway.},
  author       = {Khanam, Shakhina and Bauters, Lander and Singh, Richard Raj and Verbeek, Ruben and Haeck, Ashley and Sultan, Saeed MD and Demeestere, Kristof and Kyndt, Tina and Gheysen, Godelieve},
  issn         = {1464-6722},
  journal      = {MOLECULAR PLANT PATHOLOGY},
  keyword      = {defence hormones,Ditylenchus angustus,lignin,Manikpukha,resistance,ROOT-KNOT NEMATODE,PLANT-PARASITIC NEMATODES,RADOPHOLUS-SIMILIS INFECTION,MELOIDOGYNE-GRAMINICOLA,JASMONATE PATHWAY,CYST-NEMATODE,UFRA DISEASE,PHENYLPROPANOID METABOLISM,HYPERSENSITIVE RESPONSE,ARABIDOPSIS-THALIANA},
  language     = {eng},
  number       = {6},
  pages        = {1391--1402},
  title        = {Mechanisms of resistance in the rice cultivar Manikpukha to the rice stem nematode Ditylenchus angustus},
  url          = {http://dx.doi.org/10.1111/mpp.12622},
  volume       = {19},
  year         = {2018},
}

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