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Metabolic profile of zearalenone in liver microsomes from different species and its in vivo metabolism in rats and chickens using ultra high-pressure liquid chromatography-quadrupole/time-of-flight mass spectrometry

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Abstract
To explore differences of zearalenone (ZEN) metabolism between various species, phase I and II metabolism by liver microsomes of animals and human were investigated using ultra high-pressure liquid chromatography-quadrupole/time-offlight mass spectrometry (UHPLC-Q/TOF MS). A total of 24 metabolites were identified, among which 12 were reported for the first time. Reduction, hydroxylation, and glucuronidation were the major metabolic pathways of ZEN, and significant differences in various species were also observed. Reduction was the main reaction in swine and human, whereas hydroxylation was predominant in rats, chickens, goats, and cows in in vitro systems. Furthemore, in vivo metabolism of ZEN in rats and chickens was investigated, and 23 and 6 metabolites were identified in each species, respectively. Reduction, hydroxylation, and glucuronidation were the major metabolic pathways in rats, while reduction and sulfation predominated in chickens. These results further enrich the biotransformation profile of ZEN, providing a helpful reference for assessing the risks to animals and humans.
Keywords
ABSOLUTE ORAL BIOAVAILABILITY, MYCOTOXIN ZEARALENONE, PHASE-I, AROMATIC, HYDROXYLATION, CONTAMINATED MAIZE, CIS-ZEARALENONE, MCF-7 CELLS, VITRO, DEOXYNIVALENOL, BIOTRANSFORMATION, zearalenone, metabolism, biotransformation, phase I and phase II

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MLA
Yang, Shupeng, Huiyan Zhang, Feifei Sun, et al. “Metabolic Profile of Zearalenone in Liver Microsomes from Different Species and Its in Vivo Metabolism in Rats and Chickens Using Ultra High-pressure Liquid Chromatography-quadrupole/time-of-flight Mass Spectrometry.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.51 (2017): 11292–11303. Print.
APA
Yang, Shupeng, Zhang, H., Sun, F., De Ruyck, K., Zhang, J., Jin, Y., Li, Y., et al. (2017). Metabolic profile of zearalenone in liver microsomes from different species and its in vivo metabolism in rats and chickens using ultra high-pressure liquid chromatography-quadrupole/time-of-flight mass spectrometry. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 65(51), 11292–11303.
Chicago author-date
Yang, Shupeng, Huiyan Zhang, Feifei Sun, Karl De Ruyck, Jinzhen Zhang, Yue Jin, Yanshen Li, et al. 2017. “Metabolic Profile of Zearalenone in Liver Microsomes from Different Species and Its in Vivo Metabolism in Rats and Chickens Using Ultra High-pressure Liquid Chromatography-quadrupole/time-of-flight Mass Spectrometry.” Journal of Agricultural and Food Chemistry 65 (51): 11292–11303.
Chicago author-date (all authors)
Yang, Shupeng, Huiyan Zhang, Feifei Sun, Karl De Ruyck, Jinzhen Zhang, Yue Jin, Yanshen Li, Zhanhui Wang, Suxia Zhang, Sarah De Saeger, Jinhui Zhou, Yi Li, and Marthe De Boevre. 2017. “Metabolic Profile of Zearalenone in Liver Microsomes from Different Species and Its in Vivo Metabolism in Rats and Chickens Using Ultra High-pressure Liquid Chromatography-quadrupole/time-of-flight Mass Spectrometry.” Journal of Agricultural and Food Chemistry 65 (51): 11292–11303.
Vancouver
1.
Yang S, Zhang H, Sun F, De Ruyck K, Zhang J, Jin Y, et al. Metabolic profile of zearalenone in liver microsomes from different species and its in vivo metabolism in rats and chickens using ultra high-pressure liquid chromatography-quadrupole/time-of-flight mass spectrometry. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2017;65(51):11292–303.
IEEE
[1]
S. Yang et al., “Metabolic profile of zearalenone in liver microsomes from different species and its in vivo metabolism in rats and chickens using ultra high-pressure liquid chromatography-quadrupole/time-of-flight mass spectrometry,” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 65, no. 51, pp. 11292–11303, 2017.
@article{8546783,
  abstract     = {To explore differences of zearalenone (ZEN) metabolism between various species, phase I and II metabolism by liver microsomes of animals and human were investigated using ultra high-pressure liquid chromatography-quadrupole/time-offlight mass spectrometry (UHPLC-Q/TOF MS). A total of 24 metabolites were identified, among which 12 were reported for the first time. Reduction, hydroxylation, and glucuronidation were the major metabolic pathways of ZEN, and significant differences in various species were also observed. Reduction was the main reaction in swine and human, whereas hydroxylation was predominant in rats, chickens, goats, and cows in in vitro systems. Furthemore, in vivo metabolism of ZEN in rats and chickens was investigated, and 23 and 6 metabolites were identified in each species, respectively. Reduction, hydroxylation, and glucuronidation were the major metabolic pathways in rats, while reduction and sulfation predominated in chickens. These results further enrich the biotransformation profile of ZEN, providing a helpful reference for assessing the risks to animals and humans.},
  author       = {Yang, Shupeng and Zhang, Huiyan and Sun, Feifei and De Ruyck, Karl and Zhang, Jinzhen and Jin, Yue and Li, Yanshen and Wang, Zhanhui and Zhang, Suxia and De Saeger, Sarah and Zhou, Jinhui and Li, Yi and De Boevre, Marthe},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keywords     = {ABSOLUTE ORAL BIOAVAILABILITY,MYCOTOXIN ZEARALENONE,PHASE-I,AROMATIC,HYDROXYLATION,CONTAMINATED MAIZE,CIS-ZEARALENONE,MCF-7 CELLS,VITRO,DEOXYNIVALENOL,BIOTRANSFORMATION,zearalenone,metabolism,biotransformation,phase I and phase II},
  language     = {eng},
  number       = {51},
  pages        = {11292--11303},
  title        = {Metabolic profile of zearalenone in liver microsomes from different species and its in vivo metabolism in rats and chickens using ultra high-pressure liquid chromatography-quadrupole/time-of-flight mass spectrometry},
  url          = {http://dx.doi.org/10.1021/acs.jafc.7b04663},
  volume       = {65},
  year         = {2017},
}

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