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Molecular subtyping of Salmonella Typhimurium with multiplex oligonucleotide ligation-PCR (MOL-PCR)

Véronique Wuyts, Wesley Mattheus, Nancy HC Roosens, Kathleen Marchal UGent, Sophie Bertrand and Sigrid CJ De Keersmaecker (2017) Diagnostic bacteriology : methods and protocols. In Methods in Molecular Biology 1616. p.39-69
abstract
A multiplex oligonucleotide ligation-PCR (MOL-PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for characterization of pathogens and for diagnosis of genetic diseases, as it allows one to combine different types of molecular markers in a high-throughput multiplex assay. A MOL-PCR assay starts with a multiplex oligonucleotide ligation reaction for detection of the molecular marker, followed by a singleplex PCR for signal amplification and analysis of the MOL-PCR products on a Luminex platform. This last step occurs through a liquid bead suspension array in which the MOL-PCR products are hybridized to MagPlex-TAG beads. In this chapter, we describe the complete procedure for a MOL-PCR assay for subtyping of Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) and its monophasic variant S. 1,4[5],12:i:- from DNA isolation through heat lysis up to data interpretation through a Gödel Prime Product. The subtyping assay consists of 50 discriminative molecular markers and two internal positive control markers divided over three MOL-PCR assays.
Please use this url to cite or link to this publication:
author
organization
year
type
bookChapter
publication status
published
subject
keyword
MOL-PCR, Salmonella Typhimurium, Subtyping, Luminex, Bead suspension array, Gödel Prime Product, High-throughput assay
book title
Diagnostic bacteriology : methods and protocols
editor
Kimberly A Bishop-Lilly
series title
Methods in Molecular Biology
volume
1616
pages
39 - 69
publisher
Springer Humana Press
place of publication
New York, NY, USA
ISSN
1064-3745
1940-6029
ISBN
9781493970353
9781493970377
DOI
10.1007/978-1-4939-7037-7_3
project
Bioinformatics: from nucleotids to networks (N2N)
language
English
UGent publication?
yes
classification
B2
copyright statement
I have transferred the copyright for this publication to the publisher
id
8546637
handle
http://hdl.handle.net/1854/LU-8546637
date created
2018-01-30 06:24:49
date last changed
2018-02-28 09:31:42
@incollection{8546637,
  abstract     = {A multiplex oligonucleotide ligation-PCR (MOL-PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for characterization of pathogens and for diagnosis of genetic diseases, as it allows one to combine different types of molecular markers in a high-throughput multiplex assay. A MOL-PCR assay starts with a multiplex oligonucleotide ligation reaction for detection of the molecular marker, followed by a singleplex PCR for signal amplification and analysis of the MOL-PCR products on a Luminex platform. This last step occurs through a liquid bead suspension array in which the MOL-PCR products are hybridized to MagPlex-TAG beads.
In this chapter, we describe the complete procedure for a MOL-PCR assay for subtyping of Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) and its monophasic variant S. 1,4[5],12:i:- from DNA isolation through heat lysis up to data interpretation through a G{\"o}del Prime Product. The subtyping assay consists of 50 discriminative molecular markers and two internal positive control markers divided over three MOL-PCR assays.},
  author       = {Wuyts, V{\'e}ronique and Mattheus, Wesley and Roosens, Nancy HC and Marchal, Kathleen and Bertrand, Sophie and De Keersmaecker, Sigrid CJ},
  booktitle    = {Diagnostic bacteriology : methods and protocols},
  editor       = {Bishop-Lilly, Kimberly A},
  isbn         = {9781493970353},
  issn         = {1064-3745},
  keyword      = {MOL-PCR,Salmonella Typhimurium,Subtyping,Luminex,Bead suspension array,G{\"o}del Prime Product,High-throughput assay},
  language     = {eng},
  pages        = {39--69},
  publisher    = {Springer Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Molecular subtyping of Salmonella Typhimurium with multiplex oligonucleotide ligation-PCR (MOL-PCR)},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7037-7\_3},
  volume       = {1616},
  year         = {2017},
}

Chicago
Wuyts, Véronique, Wesley Mattheus, Nancy HC Roosens, Kathleen Marchal, Sophie Bertrand, and Sigrid CJ De Keersmaecker. 2017. “Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide ligation-PCR (MOL-PCR).” In Diagnostic Bacteriology : Methods and Protocols, ed. Kimberly A Bishop-Lilly, 1616:39–69. New York, NY, USA: Springer Humana Press.
APA
Wuyts, V., Mattheus, W., Roosens, N. H., Marchal, K., Bertrand, S., & De Keersmaecker, S. C. (2017). Molecular subtyping of Salmonella Typhimurium with multiplex oligonucleotide ligation-PCR (MOL-PCR). In K. A. Bishop-Lilly (Ed.), Diagnostic bacteriology : methods and protocols (Vol. 1616, pp. 39–69). New York, NY, USA: Springer Humana Press.
Vancouver
1.
Wuyts V, Mattheus W, Roosens NH, Marchal K, Bertrand S, De Keersmaecker SC. Molecular subtyping of Salmonella Typhimurium with multiplex oligonucleotide ligation-PCR (MOL-PCR). In: Bishop-Lilly KA, editor. Diagnostic bacteriology : methods and protocols. New York, NY, USA: Springer Humana Press; 2017. p. 39–69.
MLA
Wuyts, Véronique, Wesley Mattheus, Nancy HC Roosens, et al. “Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide ligation-PCR (MOL-PCR).” Diagnostic Bacteriology : Methods and Protocols. Ed. Kimberly A Bishop-Lilly. Vol. 1616. New York, NY, USA: Springer Humana Press, 2017. 39–69. Print.