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Molecular processes underlying synergistic linuron mineralization in a triple-species bacterial consortium biofilm revealed by differential transcriptomics

Pieter Albers, Bram Weytjens UGent, René De Mot, Kathleen Marchal UGent and Dirk Springael (2018) MICROBIOLOGYOPEN.
abstract
The proteobacteria Variovorax sp. WDL1, Comamonas testosteroni WDL7, and Hyphomicrobium sulfonivorans WDL6 compose a triple-species consortium that synergistically degrades and grows on the phenylurea herbicide linuron. To acquire a better insight into the interactions between the consortium members and the underlying molecular mechanisms, we compared the transcriptomes of the key biodegrading strains WDL7 and WDL1 grown as biofilms in either isolation or consortium conditions by differential RNAseq analysis. Differentially expressed pathways and cellular systems were inferred using the network-based algorithm PheNetic. Coculturing affected mainly metabolism in WDL1. Significantly enhanced expression of hylA encoding linuron hydrolase was observed. Moreover, differential expression of several pathways involved in carbohydrate, amino acid, nitrogen, and sulfur metabolism was observed indicating that WDL1 gains carbon and energy from linuron indirectly by consuming excretion products from WDL7 and/or WDL6. Moreover, in consortium conditions, WDL1 showed a pronounced stress response and overexpression of cell to cell interaction systems such as quorum sensing, contact-dependent inhibition, and Type VI secretion. Since the latter two systems can mediate interference competition, it prompts the question if synergistic linuron degradation is the result of true adaptive cooperation or rather a facultative interaction between bacteria that coincidentally occupy complementary metabolic niches.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
in press
subject
journal title
MICROBIOLOGYOPEN
MicrobiologyOpen
ISSN
2045-8827
DOI
10.1002/mbo3.559
project
Bioinformatics: from nucleotids to networks (N2N)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8546633
handle
http://hdl.handle.net/1854/LU-8546633
date created
2018-01-30 06:17:57
date last changed
2018-02-28 09:21:27
@article{8546633,
  abstract     = {The proteobacteria Variovorax sp. WDL1, Comamonas testosteroni WDL7, and Hyphomicrobium sulfonivorans WDL6 compose a triple-species consortium that synergistically degrades and grows on the phenylurea herbicide linuron. To acquire a better insight into the interactions between the consortium members and the underlying molecular mechanisms, we compared the transcriptomes of the key biodegrading strains WDL7 and WDL1 grown as biofilms in either isolation or consortium conditions by differential RNAseq analysis. Differentially expressed pathways and cellular systems were inferred using the network-based algorithm PheNetic. Coculturing affected mainly metabolism in WDL1. Significantly enhanced expression of hylA encoding linuron hydrolase was observed. Moreover, differential expression of several pathways involved in carbohydrate, amino acid, nitrogen, and sulfur metabolism was observed indicating that WDL1 gains carbon and energy from linuron indirectly by consuming excretion products from WDL7 and/or WDL6. Moreover, in consortium conditions, WDL1 showed a pronounced stress response and overexpression of cell to cell interaction systems such as quorum sensing, contact-dependent inhibition, and Type VI secretion. Since the latter two systems can mediate interference competition, it prompts the question if synergistic linuron degradation is the result of true adaptive cooperation or rather a facultative interaction between bacteria that coincidentally occupy complementary metabolic niches.},
  author       = {Albers, Pieter and Weytjens, Bram and De Mot, Ren{\'e} and Marchal, Kathleen and Springael, Dirk},
  issn         = {2045-8827},
  journal      = {MICROBIOLOGYOPEN},
  language     = {eng},
  title        = {Molecular processes underlying synergistic linuron mineralization in a triple-species bacterial consortium biofilm revealed by differential transcriptomics},
  url          = {http://dx.doi.org/10.1002/mbo3.559},
  year         = {2018},
}

Chicago
Albers, Pieter, Bram Weytjens, René De Mot, Kathleen Marchal, and Dirk Springael. 2018. “Molecular Processes Underlying Synergistic Linuron Mineralization in a Triple-species Bacterial Consortium Biofilm Revealed by Differential Transcriptomics.” Microbiologyopen.
APA
Albers, P., Weytjens, B., De Mot, R., Marchal, K., & Springael, D. (2018). Molecular processes underlying synergistic linuron mineralization in a triple-species bacterial consortium biofilm revealed by differential transcriptomics. MICROBIOLOGYOPEN.
Vancouver
1.
Albers P, Weytjens B, De Mot R, Marchal K, Springael D. Molecular processes underlying synergistic linuron mineralization in a triple-species bacterial consortium biofilm revealed by differential transcriptomics. MICROBIOLOGYOPEN. 2018;
MLA
Albers, Pieter, Bram Weytjens, René De Mot, et al. “Molecular Processes Underlying Synergistic Linuron Mineralization in a Triple-species Bacterial Consortium Biofilm Revealed by Differential Transcriptomics.” MICROBIOLOGYOPEN (2018): n. pag. Print.