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Nictaba homologs from Arabidopsis thaliana are involved in plant stress responses

Lore Eggermont, Karolina Stefanowicz and Els Van Damme UGent (2018) FRONTIERS IN PLANT SCIENCE. 8.
abstract
Plants are constantly exposed to a wide range of environmental stresses, but evolved complicated adaptive and defense mechanisms which allow them to survive in unfavorable conditions. These mechanisms protect and defend plants by using different immune receptors located either at the cell surface or in the cytoplasmic compartment. Lectins or carbohydrate-binding proteins are widespread in the plant kingdom and constitute an important part of these immune receptors. In the past years, lectin research has focused on the stress-inducible lectins. The Nicotiana tabacum agglutinin, abbreviated as Nictaba, served as a model for one family of stress-related lectins. Here we focus on three non-chimeric Nictaba homologs from Arabidopsis thaliana, referred to as AN3, AN4, and AN5. Confocal microscopy of ArathNictaba enhanced green fluorescent protein (EGFP) fusion constructs transiently expressed in N. benthamiana or stably expressed in A. thaliana yielded fluorescence for AN4 and AN5 in the nucleus and the cytoplasm of the plant cell, while fluorescence for AN3 was only detected in the cytoplasm. RT-qPCR analysis revealed low expression for all three ArathNictabas in different tissues throughout plant development. Stress application altered the expression levels, but all three ArathNictabas showed a different expression pattern. Pseudomonas syringae infection experiments with AN4 and AN5 overexpression lines demonstrated a significantly higher tolerance of several transgenic lines to P. syringae compared to wild type plants. Finally, AN4 was shown to interact with two enzymes involved in plant defense, namely TGG1 and BGLU23. Taken together, our data suggest that the ArathNictabas represent stress-regulated proteins with a possible role in plant stress responses. On the long term this research can contribute to the development of more stress-resistant plants.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
NUCLEAR-LOCALIZATION SIGNALS, F-BOX PROTEIN, NICOTIANA-TABACUM, TOBACCO, LECTIN, ER-BODY, BETA-GLUCOSIDASE, INDUCED EXPRESSION, EFFECTOR, PROTEINS, HISTONE PROTEINS, GENE-EXPRESSION, plant lectin, Nictaba homolog, Arabidopsis thaliana, ArathNictaba, abiotic stress, biotic stress, interaction partner, plant defense
journal title
FRONTIERS IN PLANT SCIENCE
Front. Plant Sci.
volume
8
article number
2218
pages
22 pages
Web of Science type
Article
Web of Science id
000419692900002
ISSN
1664-462X
DOI
10.3389/fpls.2017.02218
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8546384
handle
http://hdl.handle.net/1854/LU-8546384
date created
2018-01-27 17:23:57
date last changed
2018-01-30 12:57:28
@article{8546384,
  abstract     = {Plants are constantly exposed to a wide range of environmental stresses, but evolved complicated adaptive and defense mechanisms which allow them to survive in unfavorable conditions. These mechanisms protect and defend plants by using different immune receptors located either at the cell surface or in the cytoplasmic compartment. Lectins or carbohydrate-binding proteins are widespread in the plant kingdom and constitute an important part of these immune receptors. In the past years, lectin research has focused on the stress-inducible lectins. The Nicotiana tabacum agglutinin, abbreviated as Nictaba, served as a model for one family of stress-related lectins. Here we focus on three non-chimeric Nictaba homologs from Arabidopsis thaliana, referred to as AN3, AN4, and AN5. Confocal microscopy of ArathNictaba enhanced green fluorescent protein (EGFP) fusion constructs transiently expressed in N. benthamiana or stably expressed in A. thaliana yielded fluorescence for AN4 and AN5 in the nucleus and the cytoplasm of the plant cell, while fluorescence for AN3 was only detected in the cytoplasm. RT-qPCR analysis revealed low expression for all three ArathNictabas in different tissues throughout plant development. Stress application altered the expression levels, but all three ArathNictabas showed a different expression pattern. Pseudomonas syringae infection experiments with AN4 and AN5 overexpression lines demonstrated a significantly higher tolerance of several transgenic lines to P. syringae compared to wild type plants. Finally, AN4 was shown to interact with two enzymes involved in plant defense, namely TGG1 and BGLU23. Taken together, our data suggest that the ArathNictabas represent stress-regulated proteins with a possible role in plant stress responses. On the long term this research can contribute to the development of more stress-resistant plants.},
  articleno    = {2218},
  author       = {Eggermont, Lore and Stefanowicz, Karolina and Van Damme, Els},
  issn         = {1664-462X},
  journal      = {FRONTIERS IN PLANT SCIENCE},
  keyword      = {NUCLEAR-LOCALIZATION SIGNALS,F-BOX PROTEIN,NICOTIANA-TABACUM,TOBACCO,LECTIN,ER-BODY,BETA-GLUCOSIDASE,INDUCED EXPRESSION,EFFECTOR,PROTEINS,HISTONE PROTEINS,GENE-EXPRESSION,plant lectin,Nictaba homolog,Arabidopsis thaliana,ArathNictaba,abiotic stress,biotic stress,interaction partner,plant defense},
  language     = {eng},
  pages        = {22},
  title        = {Nictaba homologs from Arabidopsis thaliana are involved in plant stress responses},
  url          = {http://dx.doi.org/10.3389/fpls.2017.02218},
  volume       = {8},
  year         = {2018},
}

Chicago
Eggermont, Lore, Karolina Stefanowicz, and Els Van Damme. 2018. “Nictaba Homologs from Arabidopsis Thaliana Are Involved in Plant Stress Responses.” Frontiers in Plant Science 8.
APA
Eggermont, L., Stefanowicz, K., & Van Damme, E. (2018). Nictaba homologs from Arabidopsis thaliana are involved in plant stress responses. FRONTIERS IN PLANT SCIENCE, 8.
Vancouver
1.
Eggermont L, Stefanowicz K, Van Damme E. Nictaba homologs from Arabidopsis thaliana are involved in plant stress responses. FRONTIERS IN PLANT SCIENCE. 2018;8.
MLA
Eggermont, Lore, Karolina Stefanowicz, and Els Van Damme. “Nictaba Homologs from Arabidopsis Thaliana Are Involved in Plant Stress Responses.” FRONTIERS IN PLANT SCIENCE 8 (2018): n. pag. Print.