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Characterisation and genome sequence of the lytic Acinetobacter baumannii bacteriophage vB_AbaS_Loki

(2017) PLOS ONE. 12(2).
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Abstract
Acinetobacter baumannii has emerged as an important nosocomial pathogen in healthcare and community settings. While over 100 of Acinetobacter phages have been described in the literature, relatively few have been sequenced. This work describes the characterisation and genome annotation of a new lytic Acinetobacter siphovirus, vB_AbaS_Loki, isolated from activated sewage sludge. Sequencing revealed that Loki encapsulates a 41,308 bp genome, encoding 51 predicted open reading frames. Loki is most closely related to Acinetobacter phage IME_AB3 and more distantly related to Burkholderia phage KL1, Paracoccus phage vB_PmaS_IMEP1 and Pseudomonas phages vB_Pae_Kakheti25, vB_PaeS_SCH_Ab26 and PA73. Loki is characterised by a narrow host range, among the 40 Acinetobacter isolates tested, productive infection was only observed for the propagating host, A. baumannii ATCC 17978. Plaque formation was found to be dependent upon the presence of Ca2+ ions and adsorption to host cells was abolished upon incubation with a mutant of ATCC 17978 encoding a premature stop codon in lpxA. The complete genome sequence of vB_AbaS_Loki was deposited in the European Nucleotide Archive (ENA) under the accession number LN890663.
Keywords
PROGRAMMED TRANSLATIONAL FRAMESHIFT, PSEUDOMONAS-AERUGINOSA, COLISTIN RESISTANCE, CRYSTAL-STRUCTURE, ESCHERICHIA-COLI, TAILED PHAGES, PROTEIN, INFECTION, IDENTIFICATION, MECHANISMS

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Citation

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MLA
Turner, Dann, et al. “Characterisation and Genome Sequence of the Lytic Acinetobacter Baumannii Bacteriophage VB_AbaS_Loki.” PLOS ONE, vol. 12, no. 2, 2017, doi:10.1371/journal.pone.0172303.
APA
Turner, D., Wand, M. E., Briers, Y., Lavigne, R., Sutton, J. M., & Reynolds, D. M. (2017). Characterisation and genome sequence of the lytic Acinetobacter baumannii bacteriophage vB_AbaS_Loki. PLOS ONE, 12(2). https://doi.org/10.1371/journal.pone.0172303
Chicago author-date
Turner, Dann, Matthew E Wand, Yves Briers, Rob Lavigne, J Mark Sutton, and Darren M Reynolds. 2017. “Characterisation and Genome Sequence of the Lytic Acinetobacter Baumannii Bacteriophage VB_AbaS_Loki.” PLOS ONE 12 (2). https://doi.org/10.1371/journal.pone.0172303.
Chicago author-date (all authors)
Turner, Dann, Matthew E Wand, Yves Briers, Rob Lavigne, J Mark Sutton, and Darren M Reynolds. 2017. “Characterisation and Genome Sequence of the Lytic Acinetobacter Baumannii Bacteriophage VB_AbaS_Loki.” PLOS ONE 12 (2). doi:10.1371/journal.pone.0172303.
Vancouver
1.
Turner D, Wand ME, Briers Y, Lavigne R, Sutton JM, Reynolds DM. Characterisation and genome sequence of the lytic Acinetobacter baumannii bacteriophage vB_AbaS_Loki. PLOS ONE. 2017;12(2).
IEEE
[1]
D. Turner, M. E. Wand, Y. Briers, R. Lavigne, J. M. Sutton, and D. M. Reynolds, “Characterisation and genome sequence of the lytic Acinetobacter baumannii bacteriophage vB_AbaS_Loki,” PLOS ONE, vol. 12, no. 2, 2017.
@article{8545967,
  abstract     = {{Acinetobacter baumannii has emerged as an important nosocomial pathogen in healthcare and community settings. While over 100 of Acinetobacter phages have been described in the literature, relatively few have been sequenced. This work describes the characterisation and genome annotation of a new lytic Acinetobacter siphovirus, vB_AbaS_Loki, isolated from activated sewage sludge. Sequencing revealed that Loki encapsulates a 41,308 bp genome, encoding 51 predicted open reading frames. Loki is most closely related to Acinetobacter phage IME_AB3 and more distantly related to Burkholderia phage KL1, Paracoccus phage vB_PmaS_IMEP1 and Pseudomonas phages vB_Pae_Kakheti25, vB_PaeS_SCH_Ab26 and PA73. Loki is characterised by a narrow host range, among the 40 Acinetobacter isolates tested, productive infection was only observed for the propagating host, A. baumannii ATCC 17978. Plaque formation was found to be dependent upon the presence of Ca2+ ions and adsorption to host cells was abolished upon incubation with a mutant of ATCC 17978 encoding a premature stop codon in lpxA. The complete genome sequence of vB_AbaS_Loki was deposited in the European Nucleotide Archive (ENA) under the accession number LN890663.}},
  articleno    = {{e0172303}},
  author       = {{Turner, Dann and Wand, Matthew E and Briers, Yves and Lavigne, Rob and Sutton, J Mark and Reynolds, Darren M}},
  issn         = {{1932-6203}},
  journal      = {{PLOS ONE}},
  keywords     = {{PROGRAMMED TRANSLATIONAL FRAMESHIFT,PSEUDOMONAS-AERUGINOSA,COLISTIN RESISTANCE,CRYSTAL-STRUCTURE,ESCHERICHIA-COLI,TAILED PHAGES,PROTEIN,INFECTION,IDENTIFICATION,MECHANISMS}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{19}},
  title        = {{Characterisation and genome sequence of the lytic Acinetobacter baumannii bacteriophage vB_AbaS_Loki}},
  url          = {{http://doi.org/10.1371/journal.pone.0172303}},
  volume       = {{12}},
  year         = {{2017}},
}

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