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Comparative analysis of the immune responses induced by native versus recombinant versions of the ASP-based vaccine against the bovine intestinal parasite Cooperia oncophora

Ana González Hernández (UGent) , Jimmy Borloo (UGent) , Iris Peelaers (UGent) , Stijn Casaert (UGent) , Georges Leclercq (UGent) , Edwin Claerebout (UGent) and Peter Geldhof (UGent)
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Abstract
The protective capacities of a native double-domain activation-associated secreted protein (ndd-ASP)-based vaccine against the cattle intestinal nematode Cooperia oncophora has previously been demonstrated. However, protection analysis upon vaccination with a recombinantly produced antigen has never been performed. Therefore, the aim of the current study was to test the protective potential of a Pichia-produced double-domain ASP (pdd-ASP)-based vaccine against C. oncophora. Additionally, we aimed to compare the cellular and humoral mechanisms underlying the vaccine-induced responses by the native (ndd-ASP) and recombinant vaccines. Immunisation of cattle with the native C. oncophora vaccine conferred significant levels of protection after an experimental challenge infection, whereas the recombinant vaccine did not. Moreover, vaccination with ndd-ASP resulted in a higher proliferation of CD4-T cells both systemically and in the small intestinal mucosa when compared with animals vaccinated with the recombinant antigen. In terms of humoral response, although both native and recombinant vaccines induced similar levels of antibodies, animals vaccinated with the native vaccine were able to raise antibodies with greater specificity towards ndd-ASP in comparison with antibodies raised by vaccination with the recombinant vaccine, suggesting a differential immune recognition towards the ndd-ASP and pdd-ASP. Finally, the observation that animals displaying antibodies with higher percentages of recognition towards ndd-ASP also exhibited the lowest egg counts suggests a potential relationship between antibody specificity and protection.
Keywords
Activation-associated secreted proteins, Cooperia oncophora, Helminths, Immune response, Recombinant vaccine, NEMATODE OSTERTAGIA-OSTERTAGI, GASTROINTESTINAL NEMATODES, ANTHELMINTIC RESISTANCE, CALVES, ACTIVATION, PROTEINS, SINGLE, CATTLE, CELLS

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Chicago
González Hernández, Ana, Jimmy Borloo, Iris Peelaers, Stijn Casaert, Georges Leclercq, Edwin Claerebout, and Peter Geldhof. 2018. “Comparative Analysis of the Immune Responses Induced by Native Versus Recombinant Versions of the ASP-based Vaccine Against the Bovine Intestinal Parasite Cooperia Oncophora.” International Journal for Parasitology 48 (1): 41–49.
APA
González Hernández, A., Borloo, J., Peelaers, I., Casaert, S., Leclercq, G., Claerebout, E., & Geldhof, P. (2018). Comparative analysis of the immune responses induced by native versus recombinant versions of the ASP-based vaccine against the bovine intestinal parasite Cooperia oncophora. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 48(1), 41–49.
Vancouver
1.
González Hernández A, Borloo J, Peelaers I, Casaert S, Leclercq G, Claerebout E, et al. Comparative analysis of the immune responses induced by native versus recombinant versions of the ASP-based vaccine against the bovine intestinal parasite Cooperia oncophora. INTERNATIONAL JOURNAL FOR PARASITOLOGY. 2018;48(1):41–9.
MLA
González Hernández, Ana, Jimmy Borloo, Iris Peelaers, et al. “Comparative Analysis of the Immune Responses Induced by Native Versus Recombinant Versions of the ASP-based Vaccine Against the Bovine Intestinal Parasite Cooperia Oncophora.” INTERNATIONAL JOURNAL FOR PARASITOLOGY 48.1 (2018): 41–49. Print.
@article{8545523,
  abstract     = {The protective capacities of a native double-domain activation-associated secreted protein (ndd-ASP)-based vaccine against the cattle intestinal nematode Cooperia oncophora has previously been demonstrated. However, protection analysis upon vaccination with a recombinantly produced antigen has never been performed. Therefore, the aim of the current study was to test the protective potential of a Pichia-produced double-domain ASP (pdd-ASP)-based vaccine against C. oncophora. Additionally, we aimed to compare the cellular and humoral mechanisms underlying the vaccine-induced responses by the native (ndd-ASP) and recombinant vaccines. Immunisation of cattle with the native C. oncophora vaccine conferred significant levels of protection after an experimental challenge infection, whereas the recombinant vaccine did not. Moreover, vaccination with ndd-ASP resulted in a higher proliferation of CD4-T cells both systemically and in the small intestinal mucosa when compared with animals vaccinated with the recombinant antigen. In terms of humoral response, although both native and recombinant vaccines induced similar levels of antibodies, animals vaccinated with the native vaccine were able to raise antibodies with greater specificity towards ndd-ASP in comparison with antibodies raised by vaccination with the recombinant vaccine, suggesting a differential immune recognition towards the ndd-ASP and pdd-ASP. Finally, the observation that animals displaying antibodies with higher percentages of recognition towards ndd-ASP also exhibited the lowest egg counts suggests a potential relationship between antibody specificity and protection.},
  author       = {Gonz{\'a}lez Hern{\'a}ndez, Ana and Borloo, Jimmy and Peelaers, Iris and Casaert, Stijn and Leclercq, Georges and Claerebout, Edwin and Geldhof, Peter},
  issn         = {0020-7519},
  journal      = {INTERNATIONAL JOURNAL FOR PARASITOLOGY},
  language     = {eng},
  number       = {1},
  pages        = {41--49},
  title        = {Comparative analysis of the immune responses induced by native versus recombinant versions of the ASP-based vaccine against the bovine intestinal parasite Cooperia oncophora},
  url          = {http://dx.doi.org/10.1016/j.ijpara.2017.07.002},
  volume       = {48},
  year         = {2018},
}

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