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Identification of putative expansin-like genes from the pine wood nematode, Bursaphelenchus xylophilus, and evolution of the expansin gene family within the Nematoda

(2009) NEMATOLOGY. 11(3). p.355-364
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Abstract
We report the cloning and characterisation of genes encoding expansin-like proteins from the pine wood nematodes, Bursaphelenchus xylophilus and B. mucronatus. A small family of genes is present in both species and the Bursaphelenchus genes are most similar to expansins and expansin-like proteins from the potato cyst nematode Globodera rostochiensis and root-knot nematodes. Molecular modelling suggests that the genes could encode a protein with a structure similar to that of functionally characterised expansins. Expression analysis showed that the Bursaphelenchus expansin-like genes are expressed solely in the pharyngeal gland cells, implying a role in the host-parasite interaction, most likely in assisting migration through the plant. Some G. rostochiensis and root-knot nematode expansins are composed of a carbohydrate-binding domain coupled to an expansin domain but no carbohydrate binding domain is present on any of the Bursaphelenchus sequences. We suggest a model for evolution of the expansin gene family within the plant-parasitic nematodes of the Tylenchida and Aphelenchida.
Keywords
HIGH-THROUGHPUT, HETERODERA-GLYCINES, PARASITAPHELENCHIDAE, DEGRADATION, CELLULASES, molecular biology, host-parasite interactions, horizontal gene transfer, evolution, PLANT-CELL WALLS, PHYLOGENETIC-RELATIONSHIPS, PARASITISM, PROTEIN, CLONING

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Chicago
Kikuchi, Taisei, Hong-Mei Li, Nurul Karim, Malcolm W Kennedy, Maurice Moens, and John Jones. 2009. “Identification of Putative Expansin-like Genes from the Pine Wood Nematode, Bursaphelenchus Xylophilus, and Evolution of the Expansin Gene Family Within the Nematoda.” Nematology 11 (3): 355–364.
APA
Kikuchi, T., Li, H.-M., Karim, N., Kennedy, M. W., Moens, M., & Jones, J. (2009). Identification of putative expansin-like genes from the pine wood nematode, Bursaphelenchus xylophilus, and evolution of the expansin gene family within the Nematoda. NEMATOLOGY, 11(3), 355–364.
Vancouver
1.
Kikuchi T, Li H-M, Karim N, Kennedy MW, Moens M, Jones J. Identification of putative expansin-like genes from the pine wood nematode, Bursaphelenchus xylophilus, and evolution of the expansin gene family within the Nematoda. NEMATOLOGY. 2009;11(3):355–64.
MLA
Kikuchi, Taisei, Hong-Mei Li, Nurul Karim, et al. “Identification of Putative Expansin-like Genes from the Pine Wood Nematode, Bursaphelenchus Xylophilus, and Evolution of the Expansin Gene Family Within the Nematoda.” NEMATOLOGY 11.3 (2009): 355–364. Print.
@article{854543,
  abstract     = {We report the cloning and characterisation of genes encoding expansin-like proteins from the pine wood nematodes, Bursaphelenchus xylophilus and B. mucronatus. A small family of genes is present in both species and the Bursaphelenchus genes are most similar to expansins and expansin-like proteins from the potato cyst nematode Globodera rostochiensis and root-knot nematodes. Molecular modelling suggests that the genes could encode a protein with a structure similar to that of functionally characterised expansins. Expression analysis showed that the Bursaphelenchus expansin-like genes are expressed solely in the pharyngeal gland cells, implying a role in the host-parasite interaction, most likely in assisting migration through the plant. Some G. rostochiensis and root-knot nematode expansins are composed of a carbohydrate-binding domain coupled to an expansin domain but no carbohydrate binding domain is present on any of the Bursaphelenchus sequences. We suggest a model for evolution of the expansin gene family within the plant-parasitic nematodes of the Tylenchida and Aphelenchida.},
  author       = {Kikuchi, Taisei and Li, Hong-Mei and Karim, Nurul and Kennedy, Malcolm W and Moens, Maurice and Jones, John},
  issn         = {1388-5545},
  journal      = {NEMATOLOGY},
  keywords     = {HIGH-THROUGHPUT,HETERODERA-GLYCINES,PARASITAPHELENCHIDAE,DEGRADATION,CELLULASES,molecular biology,host-parasite interactions,horizontal gene transfer,evolution,PLANT-CELL WALLS,PHYLOGENETIC-RELATIONSHIPS,PARASITISM,PROTEIN,CLONING},
  language     = {eng},
  number       = {3},
  pages        = {355--364},
  title        = {Identification of putative expansin-like genes from the pine wood nematode, Bursaphelenchus xylophilus, and evolution of the expansin gene family within the Nematoda},
  url          = {http://dx.doi.org/10.1163/156854109X446953},
  volume       = {11},
  year         = {2009},
}

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