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From network to phenotype : the dynamic wiring of an Arabidopsis transcriptional network induced by osmotic stress

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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Plants have established different mechanisms to cope with environmental fluctuations and accordingly fine-tune their growth and development through the regulation of complex molecular networks. It is largely unknown how the network architectures change and what the key regulators in stress responses and plant growth are. Here, we investigated a complex, highly interconnected network of 20 Arabidopsis transcription factors (TFs) at the basis of leaf growth inhibition upon mild osmotic stress. We tracked the dynamic behavior of the stress-responsive TFs over time, showing the rapid induction following stress treatment, specifically in growing leaves. The connections between the TFs were uncovered using inducible overexpression lines and were validated with transient expression assays. This study resulted in the identification of a core network, composed of ERF6, ERF8, ERF9, ERF59, and ERF98, which is responsible for most transcriptional connections. The analyses highlight the biological function of this core network in environmental adaptation and its redundancy. Finally, a phenotypic analysis of loss-of-function and gain-of-function lines of the transcription factors established multiple connections between the stress-responsive network and leaf growth.
Keywords
growth regulation, mild osmotic stress, short-term stress response, transcription factors, transcriptional network, GENE REGULATORY NETWORKS, GENOME-WIDE ANALYSIS, CIRCADIAN CLOCK, LEAF DEVELOPMENT, ANALYSIS REVEALS, GROWTH, EXPRESSION, THALIANA, GIBBERELLIN, TOLERANCE

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  • Van den Broeck et al. (2017) Molecular Systems Biology 13,961.pdf
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Citation

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Chicago
Van den Broeck, Lisa, Marieke Dubois, Mattias Vermeersch, Veronique Storme, Minami Matsui, and Dirk Inzé. 2017. “From Network to Phenotype : the Dynamic Wiring of an Arabidopsis Transcriptional Network Induced by Osmotic Stress.” Molecular Systems Biology 13 (12).
APA
Van den Broeck, Lisa, Dubois, M., Vermeersch, M., Storme, V., Matsui, M., & Inzé, D. (2017). From network to phenotype : the dynamic wiring of an Arabidopsis transcriptional network induced by osmotic stress. MOLECULAR SYSTEMS BIOLOGY, 13(12).
Vancouver
1.
Van den Broeck L, Dubois M, Vermeersch M, Storme V, Matsui M, Inzé D. From network to phenotype : the dynamic wiring of an Arabidopsis transcriptional network induced by osmotic stress. MOLECULAR SYSTEMS BIOLOGY. 2017;13(12).
MLA
Van den Broeck, Lisa, Marieke Dubois, Mattias Vermeersch, et al. “From Network to Phenotype : the Dynamic Wiring of an Arabidopsis Transcriptional Network Induced by Osmotic Stress.” MOLECULAR SYSTEMS BIOLOGY 13.12 (2017): n. pag. Print.
@article{8545420,
  abstract     = {Plants have established different mechanisms to cope with environmental fluctuations and accordingly fine-tune their growth and development through the regulation of complex molecular networks. It is largely unknown how the network architectures change and what the key regulators in stress responses and plant growth are. Here, we investigated a complex, highly interconnected network of 20 Arabidopsis transcription factors (TFs) at the basis of leaf growth inhibition upon mild osmotic stress. We tracked the dynamic behavior of the stress-responsive TFs over time, showing the rapid induction following stress treatment, specifically in growing leaves. The connections between the TFs were uncovered using inducible overexpression lines and were validated with transient expression assays. This study resulted in the identification of a core network, composed of ERF6, ERF8, ERF9, ERF59, and ERF98, which is responsible for most transcriptional connections. The analyses highlight the biological function of this core network in environmental adaptation and its redundancy. Finally, a phenotypic analysis of loss-of-function and gain-of-function lines of the transcription factors established multiple connections between the stress-responsive network and leaf growth.},
  articleno    = {961},
  author       = {Van den Broeck, Lisa and Dubois, Marieke and Vermeersch, Mattias and Storme, Veronique and Matsui, Minami and Inz{\'e}, Dirk},
  issn         = {1744-4292},
  journal      = {MOLECULAR SYSTEMS BIOLOGY},
  language     = {eng},
  number       = {12},
  pages        = {21},
  title        = {From network to phenotype : the dynamic wiring of an Arabidopsis transcriptional network induced by osmotic stress},
  url          = {http://dx.doi.org/10.15252/msb.20177840},
  volume       = {13},
  year         = {2017},
}

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