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Secretome analysis of breast cancer-associated adipose tissue to identify paracrine regulators of breast cancer growth

Lore Lapeire UGent, An Hendrix UGent, Evelyne Lecoutere, Mieke Van Bockstal UGent, Jo Vandesompele UGent, Dawn Maynard, Geert Braems UGent, Rudy Van den Broecke UGent, Cathérine Müller, Marc Bracke UGent, et al. (2017) ONCOTARGET. 8(29). p.47239-47249
abstract
Adipose tissue secretes a plethora of adipokines as evidenced by characterization of subcutaneous and visceral adipose tissue secretomes. However, adipose tissue composition and secretion pattern is depot and disease dependent, influencing the adipose tissue secretome. We investigated the secretome of cancer-associated adipose tissue (CAAT) explants from breast cancer patients and explored its role in breast cancer proliferation. CAAT proteins were identified by LC-MS/MS and human protein antibody arrays and stimulated proliferation of three breast cancer cell lines. Kinomics and transcriptomics of MCF-7 breast cancer cells treated with the secretome of CAAT revealed activation of Akt-, ERK- and JNK-pathways and differential expression of activator protein 1 (AP-1) and cAMP responsive element-binding protein (CREB) target genes. The cyclin-dependent kinase (CDK) 4/6-inhibitor palbociclib significantly abrogated CAAT-enhanced breast cancer cell proliferation. Our work characterizes the specific breast CAAT protein secretome and reveals its pro-proliferative potency in breast cancer.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
adipose tissue, breast cancer, proliferation, secretome, palbociclib, DEPOT-SPECIFIC DIFFERENCES, GENE-EXPRESSION, CELL-CYCLE, INHIBITS PROLIFERATION, VISCERAL-ADIPOSE, IN-VITRO, PROGRESSION, ADIPOCYTE, APOPTOSIS, PATHWAYS
journal title
ONCOTARGET
Oncotarget
volume
8
issue
29
pages
47239 - 47249
Web of Science id
000405694000045
ISSN
1949-2553
DOI
10.18632/oncotarget.17592
project
The ignition of supernova explosions and neutrino nucleo synthesis.
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8545365
handle
http://hdl.handle.net/1854/LU-8545365
date created
2018-01-18 10:03:54
date last changed
2018-01-23 11:52:41
@article{8545365,
  abstract     = {Adipose tissue secretes a plethora of adipokines as evidenced by characterization of subcutaneous and visceral adipose tissue secretomes. However, adipose tissue composition and secretion pattern is depot and disease dependent, influencing the adipose tissue secretome. We investigated the secretome of cancer-associated adipose tissue (CAAT) explants from breast cancer patients and explored its role in breast cancer proliferation. CAAT proteins were identified by LC-MS/MS and human protein antibody arrays and stimulated proliferation of three breast cancer cell lines. Kinomics and transcriptomics of MCF-7 breast cancer cells treated with the secretome of CAAT revealed activation of Akt-, ERK- and JNK-pathways and differential expression of activator protein 1 (AP-1) and cAMP responsive element-binding protein (CREB) target genes. The cyclin-dependent kinase (CDK) 4/6-inhibitor palbociclib significantly abrogated CAAT-enhanced breast cancer cell proliferation. Our work characterizes the specific breast CAAT protein secretome and reveals its pro-proliferative potency in breast cancer.},
  author       = {Lapeire, Lore and Hendrix, An and Lecoutere, Evelyne and Van Bockstal, Mieke and Vandesompele, Jo and Maynard, Dawn and Braems, Geert and Van den Broecke, Rudy and M{\"u}ller, Cath{\'e}rine and Bracke, Marc and Cocquyt, Veronique and Denys, Hannelore and De Wever, Olivier},
  issn         = {1949-2553},
  journal      = {ONCOTARGET},
  keyword      = {adipose tissue,breast cancer,proliferation,secretome,palbociclib,DEPOT-SPECIFIC DIFFERENCES,GENE-EXPRESSION,CELL-CYCLE,INHIBITS PROLIFERATION,VISCERAL-ADIPOSE,IN-VITRO,PROGRESSION,ADIPOCYTE,APOPTOSIS,PATHWAYS},
  language     = {eng},
  number       = {29},
  pages        = {47239--47249},
  title        = {Secretome analysis of breast cancer-associated adipose tissue to identify paracrine regulators of breast cancer growth},
  url          = {http://dx.doi.org/10.18632/oncotarget.17592},
  volume       = {8},
  year         = {2017},
}

Chicago
Lapeire, Lore, An Hendrix, Evelyne Lecoutere, Mieke Van Bockstal, Jo Vandesompele, Dawn Maynard, Geert Braems, et al. 2017. “Secretome Analysis of Breast Cancer-associated Adipose Tissue to Identify Paracrine Regulators of Breast Cancer Growth.” Oncotarget 8 (29): 47239–47249.
APA
Lapeire, Lore, Hendrix, A., Lecoutere, E., Van Bockstal, M., Vandesompele, J., Maynard, D., Braems, G., et al. (2017). Secretome analysis of breast cancer-associated adipose tissue to identify paracrine regulators of breast cancer growth. ONCOTARGET, 8(29), 47239–47249.
Vancouver
1.
Lapeire L, Hendrix A, Lecoutere E, Van Bockstal M, Vandesompele J, Maynard D, et al. Secretome analysis of breast cancer-associated adipose tissue to identify paracrine regulators of breast cancer growth. ONCOTARGET. 2017;8(29):47239–49.
MLA
Lapeire, Lore, An Hendrix, Evelyne Lecoutere, et al. “Secretome Analysis of Breast Cancer-associated Adipose Tissue to Identify Paracrine Regulators of Breast Cancer Growth.” ONCOTARGET 8.29 (2017): 47239–47249. Print.