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Secretome analysis of breast cancer-associated adipose tissue to identify paracrine regulators of breast cancer growth

(2017) ONCOTARGET. 8(29). p.47239-47249
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Abstract
Adipose tissue secretes a plethora of adipokines as evidenced by characterization of subcutaneous and visceral adipose tissue secretomes. However, adipose tissue composition and secretion pattern is depot and disease dependent, influencing the adipose tissue secretome. We investigated the secretome of cancer-associated adipose tissue (CAAT) explants from breast cancer patients and explored its role in breast cancer proliferation. CAAT proteins were identified by LC-MS/MS and human protein antibody arrays and stimulated proliferation of three breast cancer cell lines. Kinomics and transcriptomics of MCF-7 breast cancer cells treated with the secretome of CAAT revealed activation of Akt-, ERK- and JNK-pathways and differential expression of activator protein 1 (AP-1) and cAMP responsive element-binding protein (CREB) target genes. The cyclin-dependent kinase (CDK) 4/6-inhibitor palbociclib significantly abrogated CAAT-enhanced breast cancer cell proliferation. Our work characterizes the specific breast CAAT protein secretome and reveals its pro-proliferative potency in breast cancer.
Keywords
adipose tissue, breast cancer, proliferation, secretome, palbociclib, DEPOT-SPECIFIC DIFFERENCES, GENE-EXPRESSION, CELL-CYCLE, INHIBITS PROLIFERATION, VISCERAL-ADIPOSE, IN-VITRO, PROGRESSION, ADIPOCYTE, APOPTOSIS, PATHWAYS

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Citation

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Chicago
Lapeire, Lore, An Hendrix, Evelyne Lecoutere, Mieke Van Bockstal, Jo Vandesompele, Dawn Maynard, Geert Braems, et al. 2017. “Secretome Analysis of Breast Cancer-associated Adipose Tissue to Identify Paracrine Regulators of Breast Cancer Growth.” Oncotarget 8 (29): 47239–47249.
APA
Lapeire, Lore, Hendrix, A., Lecoutere, E., Van Bockstal, M., Vandesompele, J., Maynard, D., Braems, G., et al. (2017). Secretome analysis of breast cancer-associated adipose tissue to identify paracrine regulators of breast cancer growth. ONCOTARGET, 8(29), 47239–47249.
Vancouver
1.
Lapeire L, Hendrix A, Lecoutere E, Van Bockstal M, Vandesompele J, Maynard D, et al. Secretome analysis of breast cancer-associated adipose tissue to identify paracrine regulators of breast cancer growth. ONCOTARGET. 2017;8(29):47239–49.
MLA
Lapeire, Lore, An Hendrix, Evelyne Lecoutere, et al. “Secretome Analysis of Breast Cancer-associated Adipose Tissue to Identify Paracrine Regulators of Breast Cancer Growth.” ONCOTARGET 8.29 (2017): 47239–47249. Print.
@article{8545365,
  abstract     = {Adipose tissue secretes a plethora of adipokines as evidenced by characterization of subcutaneous and visceral adipose tissue secretomes. However, adipose tissue composition and secretion pattern is depot and disease dependent, influencing the adipose tissue secretome. We investigated the secretome of cancer-associated adipose tissue (CAAT) explants from breast cancer patients and explored its role in breast cancer proliferation. CAAT proteins were identified by LC-MS/MS and human protein antibody arrays and stimulated proliferation of three breast cancer cell lines. Kinomics and transcriptomics of MCF-7 breast cancer cells treated with the secretome of CAAT revealed activation of Akt-, ERK- and JNK-pathways and differential expression of activator protein 1 (AP-1) and cAMP responsive element-binding protein (CREB) target genes. The cyclin-dependent kinase (CDK) 4/6-inhibitor palbociclib significantly abrogated CAAT-enhanced breast cancer cell proliferation. Our work characterizes the specific breast CAAT protein secretome and reveals its pro-proliferative potency in breast cancer.},
  author       = {Lapeire, Lore and Hendrix, An and Lecoutere, Evelyne and Van Bockstal, Mieke and Vandesompele, Jo and Maynard, Dawn and Braems, Geert and Van den Broecke, Rudy and M{\"u}ller, Cath{\'e}rine and Bracke, Marc and Cocquyt, Veronique and Denys, Hannelore and De Wever, Olivier},
  issn         = {1949-2553},
  journal      = {ONCOTARGET},
  keyword      = {adipose tissue,breast cancer,proliferation,secretome,palbociclib,DEPOT-SPECIFIC DIFFERENCES,GENE-EXPRESSION,CELL-CYCLE,INHIBITS PROLIFERATION,VISCERAL-ADIPOSE,IN-VITRO,PROGRESSION,ADIPOCYTE,APOPTOSIS,PATHWAYS},
  language     = {eng},
  number       = {29},
  pages        = {47239--47249},
  title        = {Secretome analysis of breast cancer-associated adipose tissue to identify paracrine regulators of breast cancer growth},
  url          = {http://dx.doi.org/10.18632/oncotarget.17592},
  volume       = {8},
  year         = {2017},
}

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